Results 51 to 60 of about 436,281 (168)

From bench to bytes: a practical guide to RNA sequencing data analysis

Frontiers in Genetics
RNA sequencing (RNA-Seq) is a high-throughput sequencing approach that enables comprehensive quantification of transcriptomes at a genome-wide scale. As a result, RNA-Seq has become a routine component of molecular biology research, and more researchers ...
Prabin Dawadi, Bivek Pokharel, Anita Shrestha, Dikshya Niraula, Afifa Naeem, Sayaka Miura, Mishal Roy, Saroj Nepal   +7 more
doaj   +1 more source

Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers

BMC Genomics, 2018
Background RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting material, but not every molecule ...
Yu Fu, Pei-Hsuan Wu, Timothy Beane, Phillip D. Zamore, Zhiping Weng   +4 more
doaj   +1 more source

Pan‐Cancer Single‐Nucleus Total RNA Sequencing Using snHH‐Seq

Advanced Science
Tumor heterogeneity and its drivers impair tumor progression and cancer therapy. Single‐cell RNA sequencing is used to investigate the heterogeneity of tumor ecosystems. However, most methods of scRNA‐seq amplify the termini of polyadenylated transcripts,
Haide Chen, Xiunan Fang, Jikai Shao, Qi Zhang, Liwei Xu, Jiaye Chen, Yuqing Mei, Mengmeng Jiang, Yuting Wang, Zhouyang Li, Zihang Chen, Yang Chen, Chengxuan Yu, Lifeng Ma, Peijing Zhang, Tianyu Zhang, Yuan Liao, Yuexiao Lv, Xueyi Wang, Lei Yang, Yuting Fu, Daobao Chen, Liming Jiang, Feng Yan, Wei Lu, Gao Chen, Huahao Shen, Jingjing Wang, Changchun Wang, Tingbo Liang, Xiaoping Han, Yongcheng Wang, Guoji Guo   +32 more
doaj   +1 more source

RNA sequencing and immunohistochemistry jointly improve tumor biomarker interpretation

Scientific Reports
This study aimed to assess the correlation between RNA sequencing (RNA-seq) and immunohistochemistry (IHC) in detecting key cancer biomarkers across solid tumors, and then, to establish RNA-seq thresholds that accurately reflect clinical IHC ...
Vladimir Kushnarev, Danil Stupichev, Suren Davitavyan, Kirill Kriukov, Basavaraja U. Shanthappa, Anna Butusova, Sofia Menshikova, Anna Belozerova, Anastasia Shvyrkova, Arina Tkachuk, Olga Khatenkova, Linda Balabanian, Ekaterina Postovalova, Jochen K. Lennerz, Funda Meric-Bernstam, Alexander Bagaev   +15 more
doaj   +1 more source

SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology

Genome Biology, 2018
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq).
Mahmoud N. Abdelmoez, Kei Iida, Yusuke Oguchi, Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G. Santiago, Hirofumi Shintaku   +8 more
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RNA-Seq Illumina

, 2020
RNA-Seq with ...
openaire   +2 more sources

MOUtbhR RNA-seq

, 2017
RNA-seq pipeline analysis of mouse single end libraries.
Manchon, Laurent, TAZI, Jamal
openaire   +1 more source

Poly(A) inclusive RNA isoform sequencing (PAIso−seq) reveals wide-spread non-adenosine residues within RNA poly(A) tails

Nature Communications, 2019
The poly(A) tails on mRNA are vital for their function but it is difficult to map full-length sequences of mRNA isoforms with the entire poly(A) tails.
Yusheng Liu, Hu Nie, Hongxiang Liu, Falong Lu   +3 more
doaj   +1 more source

Single-cell total-RNA profiling unveils regulatory hubs of transcription factors

Nature Communications
Recent development of RNA velocity uses master equations to establish the kinetics of the life cycle of RNAs from unspliced RNA to spliced RNA (i.e., mature RNA) to degradation. To feed this kinetic analysis, simultaneous measurement of unspliced RNA and
Yichi Niu, Jiayi Luo, Chenghang Zong
doaj   +1 more source

A bifunctional snoRNA with separable activities in guiding rRNA 2’-O-methylation and scaffolding gametogenesis effectors

Nature Communications
Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels.
Estelle Leroy, Drice Challal, Stéphane Pelletier, Coralie Goncalves, Alexandra Menant, Virginie Marchand, Yan Jaszczyszyn, Erwin van Dijk, Delphine Naquin, Jessica Andreani, Yuri Motorin, Benoit Palancade, Mathieu Rougemaille   +12 more
doaj   +1 more source