Results 81 to 90 of about 536,139 (245)

An observation of circular RNAs in bacterial RNA-seq data [PDF]

, 2016
Circular RNAs (circRNAs) are a class of RNA with an important role in micro RNA (miRNA) regulation recently discovered in Human and various other eukaryotes as well as in archaea.
Aurell, Erik, d'hérouël, Aymeric Fouquier, Innocenti, Nicolas, Nguyen, Hoang-Son   +3 more
core   +1 more source

A Comparison of RNA-Seq and Exon Arrays for Whole Genome Transcription Profiling of the L5 Spinal Nerve Transection Model of Neuropathic Pain in the Rat [PDF]

, 2014
BACKGROUND: The past decade has seen an abundance of transcriptional profiling studies of preclinical models of persistent pain, predominantly employing microarray technology.
Ana Antunes-Martins, Christine Orengo, David LH Bennett, James R Perkins, John Grist, Margarita Calvo, Matthias Kohl, Ramona Schmid, Stephen B McMahon, Tobias Hildebrandt, Werner Rust   +10 more
core   +3 more sources

CleanUpRNAseq: An R/Bioconductor Package for Detecting and Correcting DNA Contamination in RNA-Seq Data

BioTech
RNA sequencing (RNA-seq) has become a standard method for profiling gene expression, yet genomic DNA (gDNA) contamination carried over to the sequencing library poses a significant challenge to data integrity.
Haibo Liu, Kai Hu, Kevin O’Connor, Michelle A. Kelliher, Lihua Julie Zhu   +4 more
doaj   +1 more source

Statistical calibration of qRT-PCR, microarray and RNA-Seq gene expression data with measurement error models

, 2014
The accurate quantification of gene expression levels is crucial for transcriptome study. Microarray platforms are commonly used for simultaneously interrogating thousands of genes in the past decade, and recently RNA-Seq has emerged as a promising ...
Kuczek, Thomas, Sun, Zhaonan, Zhu, Yu
core   +1 more source

Differential meta-analysis of RNA-seq data from multiple studies [PDF]

, 2013
High-throughput sequencing is now regularly used for studies of the transcriptome (RNA-seq), particularly for comparisons among experimental conditions.
Jaffrézic, Florence, Marot, Guillemette, Rau, Andrea   +2 more
core   +8 more sources

Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers

BMC Genomics, 2018
Background RNA-seq and small RNA-seq are powerful, quantitative tools to study gene regulation and function. Common high-throughput sequencing methods rely on polymerase chain reaction (PCR) to expand the starting material, but not every molecule ...
Yu Fu, Pei-Hsuan Wu, Timothy Beane, Phillip D. Zamore, Zhiping Weng   +4 more
doaj   +1 more source

Navigating in a sea of repeats in RNA-seq without drowning

, 2014
The main challenge in de novo assembly of NGS data is certainly to deal with repeats that are longer than the reads. This is particularly true for RNA- seq data, since coverage information cannot be used to flag repeated sequences, of which transposable ...
Lacroix, Vincent, Marchet, Camille, Miele, Vincent, Sacomoto, Gustavo, Sagot, Marie-France, Sinaimeri, Blerina   +5 more
core   +3 more sources

SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology

Genome Biology, 2018
We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq).
Mahmoud N. Abdelmoez, Kei Iida, Yusuke Oguchi, Hidekazu Nishikii, Ryuji Yokokawa, Hidetoshi Kotera, Sotaro Uemura, Juan G. Santiago, Hirofumi Shintaku   +8 more
doaj   +1 more source

Transcriptome Analysis Using RNA-Seq [PDF]

, 2012
Transcriptome analysis by next-generation sequencing (RNA-seq) allows investigation of a transcriptome at unsurpassed resolution. One major benefit is that RNA-seq is independent of a priori knowledge on the sequence under investigation, thereby also allowing analysis of poorly characterized Plasmodium species.
Hoeijmakers, W.A.M., Bartfai, R.C., Stunnenberg, H.   +2 more
openaire   +2 more sources

A bifunctional snoRNA with separable activities in guiding rRNA 2’-O-methylation and scaffolding gametogenesis effectors

Nature Communications
Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels.
Estelle Leroy, Drice Challal, Stéphane Pelletier, Coralie Goncalves, Alexandra Menant, Virginie Marchand, Yan Jaszczyszyn, Erwin van Dijk, Delphine Naquin, Jessica Andreani, Yuri Motorin, Benoit Palancade, Mathieu Rougemaille   +12 more
doaj   +1 more source