Results 81 to 90 of about 567,059 (348)

ROCK signalling induced gene expression changes in mouse pancreatic ductal adenocarcinoma cells [PDF]

, 2016
The RhoA and RhoC GTPases act via the ROCK1 and ROCK2 kinases to promote actomyosin contraction, resulting in directly induced changes in cytoskeleton structures and altered gene transcription via several possible indirect routes.
Clark, William, Kalna, Gabriela, Olson, Michael F., Rath, Nicola   +3 more
core   +1 more source

Supersplat—spliced RNA-seq alignment [PDF]

Bioinformatics, 2010
Abstract Motivation: High-throughput sequencing technologies have recently made deep interrogation of expressed transcript sequences practical, both economically and temporally. Identification of intron/exon boundaries is an essential part of genome annotation, yet remains a challenge.
Rongkun Shen, Weng-Keen Wong, Henry D. Priest, Todd C. Mockler, Douglas W. Bryant   +4 more
openaire   +3 more sources

Tumor clusters with divergent inflammation and human retroelement expression determine the clinical outcome of patients with serous ovarian cancer

Molecular Oncology, EarlyView.
Analysis of treatment‐naïve high‐grade serous ovarian carcinoma (HGSOC) and control tissues for ERVs, LINE‐1 (L1), inflammation, and immune checkpoints identified five clusters with diverse patient recurrence‐free survivals. An inflammation score was calculated and correlated with retroelement expression, where one novel cluster (Triple‐I) with high ...
Laura Glossner, Markus Eckstein, Christoph Mark, Matthias W. Beckmann, Arndt Hartmann, Pamela L. Strissel, Reiner Strick   +6 more
wiley   +1 more source

PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data

Genomics, Proteomics & Bioinformatics, 2014
The vast majority of multi-exon genes in higher eukaryotes are alternatively spliced and changes in alternative splicing (AS) can impact gene function or cause disease.
Collin Tokheim, Juw Won Park, Yi Xing
doaj   +1 more source

European sea bass (Dicentrarchus labrax) skin and scale transcriptomes [PDF]

, 2017
Fish skin and their appendages, the mineralized scales, are important organs for protection and homeostasis, but little is known about their specific transcript or protein repertoire.
Pinto, Patricia IS, Power, Deborah, Thorne, Michael A. S.   +2 more
core   +1 more source

Development and evaluation of RNA-seq methods [PDF]

Genome Biology, 2010
This article is part of the supplement: Beyond the Genome: The true gene count, human evolution and disease ...
Nir Friedman, Lin Fan, Xian Adiconis, Andreas Gnirke, Dawn Thompson, Moran Yassour, Moran Yassour, Mitchell Guttman, Michael F. Berger, Aviv Regev, Chad Nusbaum, Joshua Z. Levin   +11 more
openaire   +4 more sources

EGFR‐STAT3 activation provides a therapeutic rationale for targeting aggressive ETV1‐positive prostate cancer

Molecular Oncology, EarlyView.
Cotargeting EGFR and STAT3 with Erlotinib and TTI‐101 impairs both 2D and 3D growth of ETV1‐overexpressing prostate cancer cells by disrupting a self‐sustaining ETV1–EGFR positive feedback loop that promotes EGFR and STAT3 expression and phosphorylation (activation).
Elsa Gomes Paiva, Bernardo Orr, Ana Azeredo, Andreia Brandão, Manuel R. Teixeira, Paula Paulo   +5 more
wiley   +1 more source

Comparison of RNA-Seq and microarray in transcriptome profiling of activated T cells.

PLoS ONE, 2014
To demonstrate the benefits of RNA-Seq over microarray in transcriptome profiling, both RNA-Seq and microarray analyses were performed on RNA samples from a human T cell activation experiment.
Shanrong Zhao, Wai-Ping Fung-Leung, Anton Bittner, Karen Ngo, Xuejun Liu   +4 more
doaj   +1 more source

DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate

Plant Methods, 2022
Background Quantification of gene expression such as RNA-Seq is a popular approach to study various biological phenomena. Despite the development of RNA-Seq library preparation methods and sequencing platforms in the last decade, RNA extraction remains ...
Makoto Kashima, Mari Kamitani, Yasuyuki Nomura, Natsumi Mori-Moriyama, Shigeyuki Betsuyaku, Hiromi Hirata, Atsushi J. Nagano   +6 more
doaj   +1 more source

A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]

, 2012
With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood, Jie ePeng, Julin N Maloof, Lauren R Headland, Neelima R Sinha, Ravi eKumar, Seisuke eKimura, Seisuke eKimura, Yasunori eIchihashi   +8 more
core   +2 more sources