Results 221 to 230 of about 331,295 (252)

An assay to determine the kinetics of RNA cleavage

Analytical Biochemistry, 1985
To evaluate some synthetic catalysts that mimic ribonuclease, a quantitative assay has been developed that measures the number of phosphate diester bonds cleaved in a polymeric RNA substrate. This assay involves determining the number of 5'-oligonucleotide termini produced during the cleavage, using polyuridylic acid as the substrate. Samples withdrawn
R, Corcoran, M, Labelle, A W, Czarnik, R, Breslow   +3 more
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RNA Recognition and Cleavage by a Splicing Endonuclease

Science, 2006
The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus
Song, Xue, Kate, Calvin, Hong, Li
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Chemical Diversity in RNA Cleavage

Science, 1999
It was quite a shock when it was first discovered that RNA can perform catalysis, sometimes every bit as efficiently as an enzyme. In his Perspective, [Westhof][1] now describes new evidence ([ Perrotta et al. ][2]) that so-called ribozymes can perform base-catalysis without the assistance of a metal ion. In an early world containing only RNA molecules,
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Cleavage of RNA by Imidazole

2004
Development of RNA cleaving agents, artificial RNases, has attracted considerable attention in the last years, because they can find important applications in biotechnology, for manipulating RNA, and in drug design. A direct approach to design of artificial RNases consists in imitation of the active centers of natural enzymes by simple organic ...
V. V. Vlassov, A. V. Vlassov
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[Hybridase cleavage of RNA. I. Oligonucleotide probes for regiospecific RNA cleavage].

Bioorganicheskaia khimiia, 1990
New oligonucleotide probes for regiospecific cleavage of RNA molecules by hybridase (RNase H) are suggested. RNase H from E. coli is shown to site-specifically split eight phosphodiester bonds in RNA in the heteroduplex, formed by 5S rRNA and d(ACCACCGCGCT).
V G, Metelev, N F, Krynetskaia, A A, Purmal', Z A, Shabarova   +3 more
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The self-cleavage of lariat-RNA

Tetrahedron Letters, 1993
Abstract Lariat-RNAs 3 and 4 undergo site-specific self-cleavage reaction at the G 3 → C 6 /U 7 phosphodiester bond by the nucleophilic attack of 2′-OH of G 3 sugar moiety to its 3′-phosphate to give 5′-hydroxyl terminal at C 6 or U 7 and 2′,3′-cyclic phosphodiester of G 3 whereas lariat-tetramer 1 , pentamer 2 , the cyclic-A(2′→5′)G ...
Peter Agback, Corine Glemarec, Lee Yin, Anders Sandström, Janez Plavec, Christian Sund, Shun-ichi Yamakage, Garimella Viswanadham, Bertrand Rousse, Nitin Puri, Jyoti Chattopadhyaya   +10 more
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Self-cleavage of rna in the replication of viroids and virusoids

Journal of Cell Science, 1987
ABSTRACT Viroids are infectious, circular RNA molecules of 246 to 375 nucleotides found in plants. Virusoids are of similar size and structure but they are dependent on, and encapsidated in, a helper virus. A rolling circle mechanism of replication is considered to account for the presence of greater-than-unit-length plus and minus RNAs ...
R H, Symons, C J, Hutchins, A C, Forster, P D, Rathjen, P, Keese, J E, Visvader   +5 more
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Structural determinants of RNA recognition and cleavage by Dicer

Nature Structural & Molecular Biology, 2007
A hallmark of RNA interference is the production of short double-stranded RNA (dsRNA) molecules 21-28 nucleotides in length by the specialized RNase III protein Dicer. Dicer enzymes uniquely generate RNA products of specific lengths by mechanisms that have not been fully elucidated. Here we show that the PAZ domain responsible for dsRNA end recognition
Ian J, MacRae, Kaihong, Zhou, Jennifer A, Doudna   +2 more
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[Hybridase cleavage of RNA. V. Complementary precision of cleavage].

Molekuliarnaia biologiia, 1992
The efficiency of the cleavage of RNA involved in perfect as well as imperfect hybrid duplexes composed of three components: (1) homogeneous RNA's or polyribonucleotides; (2) corresponding complementary synthetic oligodeoxyribonucleotides; (3) E. coli RNase H was investigated.
V G, Metelev, N F, Krynetskaia, E M, Volkov, T S, Oretskaia, Z A, Shabarova   +4 more
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Structural insights into RNA cleavage by PIWI Argonaute

Nature
Argonaute proteins are categorized into AGO and PIWI clades. Across most animal species, AGO-clade proteins are widely expressed in various cell types, and regulate normal gene expression1. By contrast, PIWI-clade proteins predominantly function during gametogenesis to suppress transposons and ensure fertility1,2.
Zhiqing Li, Qikui Xu, Jing Zhong, Yan Zhang, Tianxiang Zhang, Xiaoze Ying, Xiaoli Lu, Xiaoyi Li, Li Wan, Junchao Xue, Jing Huang, Ying Zhen, Zhao Zhang, Jianping Wu, En-Zhi Shen   +14 more
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