Chemoproteomic discovery of a human RNA ligase [PDF]
Nature Communications, 2023 RNA ligases are present across all forms of life. Here, the hitherto uncharacterised human protein C12orf29 was identified as a human enzyme promoting RNA ligation between 5′-PO4 and 3′-OH termini.
Yizhi Yuan +11 more
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The RNA ligase RtcB reverses MazF-induced ribosome heterogeneity in Escherichia coli. [PDF]
Nucleic Acids Res, 2017 When Escherichia coli encounters stress, the endoribonuclease MazF initiates a post-transcriptional response that results in the reprogramming of protein synthesis.
Temmel H +7 more
europepmc +4 more sources
Functional analysis of the mammalian RNA ligase for IRE1 in the unfolded protein response. [PDF]
Biosci Rep, 2017 The unfolded protein response (UPR) is a conserved signalling pathway activated on the accumulation of unfolded proteins within the endoplasmic reticulum (ER), termed ER stress.
Poothong J, Tirasophon W, Kaufman RJ.
europepmc +4 more sources
RNA ligase ribozymes with a small catalytic core [PDF]
Scientific Reports, 2023 Catalytic RNAs, or ribozymes, catalyze diverse chemical reactions that could have sustained primordial life in the hypothetical RNA world. Many natural ribozymes and laboratory evolved ribozymes exhibit efficient catalysis mediated by elaborate catalytic
Yoko Nomura, Yohei Yokobayashi
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Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand. [PDF]
Nucleic Acids Res, 2020 Rings of single-stranded RNA are promising for many practical applications, but the methods to prepare them in preparative scale have never been established.
Chen H +5 more
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Systematic minimization of RNA ligase ribozyme through large-scale design-synthesis-sequence cycles. [PDF]
Nucleic Acids Res, 2019 Template-directed RNA ligation catalyzed by an RNA enzyme (ribozyme) is a plausible and important reaction that could have been involved in transferring genetic information during prebiotic evolution. Laboratory evolution experiments have yielded several
Nomura Y, Yokobayashi Y.
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Purification of Histidine-Tagged T4 RNA Ligase from E. coli [PDF]
BioTechniques, 2002 Here we report the construction of a histidine-tagged T4 RNA ligase expression plasmid (pRHT4). The construct, when overexpressed in BL21 (DE3) cells, allows the preparation of large quantities of T4 RNA ligase in high purity using only a single ...
Qing S. Wang, Peter J. Unrau
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Elimination of ligation dependent artifacts in T4 RNA ligase to achieve high efficiency and low bias microRNA capture. [PDF]
PLoS ONE, 2014 Adapter ligation is a critical first step in many microRNA analysis methods including microarray, qPCR, and sequencing. Previous studies have shown that ligation bias can have dramatic effects on both the fidelity of expression profiles and ...
Yunke Song, Kelvin J Liu, Tza-Huei Wang
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Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease [PDF]
Nature CommunicationsRNA ligases of the RTCB-type play an essential role in tRNA splicing, the unfolded protein response and RNA repair. RTCB is the catalytic subunit of the pentameric human tRNA ligase complex.
Janina Lara Gerber +5 more
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Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase. [PDF]
Proc Natl Acad Sci U S A, 2015 Significance Polynucleotide ligases are an ancient superfamily of nucleic acid repair enzymes that join 3′-OH and 5′-PO4 DNA or RNA ends. Ligases react with ATP or NAD+ to form a covalent enzyme–adenylate intermediate in which AMP is linked via a P–N ...
Unciuleac MC, Goldgur Y, Shuman S.
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