Chemoproteomic discovery of a human RNA ligase [PDF]
Nature Communications, 2023 RNA ligases are present across all forms of life. Here, the hitherto uncharacterised human protein C12orf29 was identified as a human enzyme promoting RNA ligation between 5′-PO4 and 3′-OH termini.
Yizhi Yuan +11 more
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RNA ligase ribozymes with a small catalytic core [PDF]
Scientific Reports, 2023 Catalytic RNAs, or ribozymes, catalyze diverse chemical reactions that could have sustained primordial life in the hypothetical RNA world. Many natural ribozymes and laboratory evolved ribozymes exhibit efficient catalysis mediated by elaborate catalytic
Yoko Nomura, Yohei Yokobayashi
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Functional analysis of the mammalian RNA ligase for IRE1 in the unfolded protein response. [PDF]
Biosci Rep, 2017 The unfolded protein response (UPR) is a conserved signalling pathway activated on the accumulation of unfolded proteins within the endoplasmic reticulum (ER), termed ER stress.
Poothong J, Tirasophon W, Kaufman RJ.
europepmc +4 more sources
Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease [PDF]
Nature CommunicationsRNA ligases of the RTCB-type play an essential role in tRNA splicing, the unfolded protein response and RNA repair. RTCB is the catalytic subunit of the pentameric human tRNA ligase complex.
Janina Lara Gerber +5 more
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Enzymatic Conjugation of Modified RNA Fragments by Ancestral RNA Ligase AncT4_2. [PDF]
Appl Environ Microbiol, 2022 RNA therapeutics is one of the next-generation medicines for treating various diseases. Our designed ancestral RNA ligase AncT4_2 exhibited excellent enzymatic properties, such as high thermal stability, productivity, specific activity, and broad ...
Kajimoto S +7 more
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High-throughput sequencing reveals circular substrates for an archaeal RNA ligase. [PDF]
RNA Biol, 2017 Becker HF +5 more
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Preferential production of RNA rings by T4 RNA ligase 2 without any splint through rational design of precursor strand. [PDF]
Nucleic Acids Res, 2020 Rings of single-stranded RNA are promising for many practical applications, but the methods to prepare them in preparative scale have never been established.
Chen H +5 more
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Systematic minimization of RNA ligase ribozyme through large-scale design-synthesis-sequence cycles. [PDF]
Nucleic Acids Res, 2019 Template-directed RNA ligation catalyzed by an RNA enzyme (ribozyme) is a plausible and important reaction that could have been involved in transferring genetic information during prebiotic evolution. Laboratory evolution experiments have yielded several
Nomura Y, Yokobayashi Y.
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RNA ligation of very small pseudo nick structures by T4 RNA ligase 2, leading to efficient production of versatile RNA rings. [PDF]
RSC Adv, 2019 T4 RNA ligase 2 catalyses two types of reactions: (i) sealing of a nick structure in double-stranded RNA and (ii) connection of two single-stranded RNA strands.
Cheng K +8 more
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An RNA ligase partner for the prokaryotic protein-only RNase P: insights into the functional diversity of RNase P from genome mining [PDF]
mBioRNase P can use either an RNA- or a protein-based active site to catalyze 5′-maturation of transfer RNAs (tRNAs). This distinctive attribute in the biocatalytic repertoire raises questions about the underlying evolutionary driving forces, especially if ...
Rekha Seshadri, Venkat Gopalan
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