Results 101 to 110 of about 1,338,157 (346)
Structural bias in T4 RNA ligase-mediated 3′-adapter ligation
Nucleic Acids Research, 2012 T4 RNA ligases are commonly used to attach adapters to RNAs, but large differences in ligation efficiency make detection and quantitation problematic.
Fanglei Zhuang +4 more
semanticscholar +1 more source
High‐Resolution Patterned Delivery of Chemical Signals From 3D‐Printed Picoliter Droplet Networks
Advanced Materials, EarlyView.3D‐printed picoliter droplet networks have been fabricated that control gene expression in bacterial populations by releasing chemical signals with precise spatial definition and high temporal resolution. This system of effector release is widely applicable, offering diverse applications in biology and medicine.
Jorin Riexinger +7 more
wiley +1 more source
Cloning Full-Length, Cap-Trapper-Selected cDNAs by Using the Single-Strand Linker Ligation Method
BioTechniques, 2001 We have developed the single-strand linker ligation method (SSLLM), which uses DNA ligase to add a dsDNA linker to singlestranded (ss) full-length cDNA. The linkers have random 6-bp (dN6 or dGN5) 3′ overhangs that can ligate to any cDNA sequence, thereby
Y. Shibata +6 more
doaj +1 more source
Force for ancient and recent life: viral and stem-loop RNA consortia promote life. [PDF]
, 2014 Lytic viruses were thought to kill the most numerous host (i.e., kill the winner). But persisting viruses/defectives can also protect against viruses, especially in a ubiquitous virosphere. In 1991, Yarmolinsky et al.
Villarreal, Luis P
core +1 more source
RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries.
RNA: A publication of the RNA Society, 2011 Sequencing of small RNA cDNA libraries is an important tool for the discovery of new RNAs and the analysis of their mutational status as well as expression changes across samples.
M. Hafner +13 more
semanticscholar +1 more source
Protein‐Like Polymers Targeting Keap1/Nrf2 as Therapeutics for Myocardial Infarction
Advanced Materials, EarlyView.We describe a Keap1 targeting protein‐like polymer (PLP) which activates Nrf2, an important cytoprotective transcription factor for relieving myocardial infarction‐induced oxidative stress. This PLP increases cell survival in vitro in multiple relevant cardiac cell types and elicits pro‐reparative responses, improving cardiac function in a preclinical ...
Joshua M. Mesfin +17 more
wiley +1 more source
Nature Communications, 2017 Stress granules (SG) comprise aggregates of cellular messenger ribonucleoproteins (mRNPs) but how they form is unclear. Here, the authors identify NEDD4, an E3 ubiquitin ligase, as regulating the RNA binding protein NANOS2 and turnover of mRNP components,
Zhi Zhou +4 more
doaj +1 more source
TRIM41 is required to innate antiviral response by polyubiquitinating BCL10 and recruiting NEMO
Signal Transduction and Targeted Therapy, 2021 Sensing of pathogenic nucleic acids by pattern recognition receptors (PRR) not only initiates anti-microbe defense but causes inflammatory and autoimmune diseases. E3 ubiquitin ligase(s) critical in innate response need to be further identified.
Zhou Yu +9 more
doaj +1 more source
DNA‑Directed Assembly of Photonic Nanomaterials for Diagnostic and Therapeutic Applications
Advanced Materials, EarlyView.DNA‐directed assembly offers a powerful strategy for constructing structured photonic nanomaterials with precise spatial control. This review provides a comprehensive overview of recent advancements in DNA‐assembled photonic nanomaterials for diagnostics and therapeutics, highlighting key design principles, functionalization strategies, and optical ...
Longjiang Ding +5 more
wiley +1 more source
Nature CommunicationsTRIM25 is an RNA-binding ubiquitin E3 ligase with central but poorly understood roles in the innate immune response to RNA viruses. The link between TRIM25’s RNA binding and its role in innate immunity has not been established.
Lucía Álvarez +20 more
doaj +1 more source