Results 111 to 120 of about 1,353,045 (362)

The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity

FEBS Open Bio, EarlyView.
We identified RNF219 as a CCR4‐NOT complex‐interacting E3 ubiquitin ligase that targets the CCR4‐NOT subunit CNOT6L for ubiquitination. RNF219 directly binds to the DUF3819 domain of CNOT1 through its putative α‐helix spanning amino acids 521–542. Our findings also suggest that antiproliferative activity of RNF219 is at least partially mediated by ...
Shou Soeda, Melissa Montrose, Akinori Takahashi, Risa Ishida, Sandrine Burriel, Nao Ohmine, Tohru Natsume, Shungo Adachi, Minsoo Kim, Tadashi Yamamoto   +9 more
wiley   +1 more source

Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts [PDF]

, 2014
Microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments.
Abou-El-Ardat, Khalil, Baatout, Sarah, Beck, Michaël, De Vos, Winnok, El-Saghire, Hussein, Janssen, Ann, Michaux, Arlette, Moreels, Marjan, Neefs, Mieke, Quintens, Roel, Tabury, Kevin, Van Oostveldt, Patric   +11 more
core   +2 more sources

RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries.

RNA: A publication of the RNA Society, 2011
Sequencing of small RNA cDNA libraries is an important tool for the discovery of new RNAs and the analysis of their mutational status as well as expression changes across samples.
M. Hafner, N. Renwick, Miguel A. Brown, Aleksandra Mihailović, D. Holoch, Carolina P. Lin, John T. G. Pena, Jeff Nusbaum, Pavel Morozov, J. Ludwig, Tolulope A. Ojo, Shujun J Luo, G. Schroth, T. Tuschl   +13 more
semanticscholar   +1 more source

Equimolar addition of oligoribonucleotides with T4 RNA ligase

Nucleic Acids Research, 1977
T4 induced RNA ligase will join equimolar concentrations of two oligoribonucleotides, (Ap)3C and p(Up) 5, to form a single product, (Ap)3Cp(Up) 5, in high yield. The presence of the 3' phosphate on p(Up)5 prevents the oligomer from adding to itself. The pH optimum of the reaction is about 7.5, but less of the undesirable adenylated intermediate, App ...
Vicki Cameron, Olke C. Uhlenbeck
openaire   +4 more sources

An RNA ligase-mediated method for the efficient creation of large, synthetic RNAs [PDF]

RNA, 2006
RNA ligation has been a powerful tool for incorporation of cross-linkers and nonnatural nucleotides into internal positions of RNA molecules. The most widely used method for template-directed RNA ligation uses DNA ligase and a DNA splint. While this method has been used successfully for many years, it suffers from a number of drawbacks, principally ...
Martha R. Stark, Jeffrey A. Pleiss, Stephen Scaringe, Stephen D. Rader, Michael L. Deras   +4 more
openaire   +3 more sources

Activation of NF‐κB Signaling by Optogenetic Clustering of IKKα and β

Advanced Biology, EarlyView.
This study presents an optogenetic approach for graded clustering of eGFP‐fused proteins using an eGFP‐specific nanobody and engineered Cryptochrome 2 variants. The method enables potent, reversible activation of NF‐κB signaling via endogenous pathways, as confirmed by RNA sequencing. This versatile system provides a spatially and temporally controlled
Alexandra Anna Maria Fischer, Markus Michael Kramer, Miguel Baños, Merlin Moritz Grimm, Manfred Fliegauf, Bodo Grimbacher, Gerald Radziwill, Sven Rahmann, Wilfried Weber   +8 more
wiley   +1 more source

Lentiviral Vpx accessory factor targets VprBP/DCAF1 substrate adaptor for cullin 4 E3 ubiquitin ligase to enable macrophage infection. [PDF]

PLoS Pathogens, 2008
Vpx is a small virion-associated adaptor protein encoded by viruses of the HIV-2/SIVsm lineage of primate lentiviruses that enables these viruses to transduce monocyte-derived cells.
Smita Srivastava, Selene K Swanson, Nicolas Manel, Laurence Florens, Michael P Washburn, Jacek Skowronski   +5 more
doaj   +1 more source

USP15 regulates dynamic protein-protein interactions of the spliceosome through deubiquitination of PRP31. [PDF]

, 2017
Post-translational modifications contribute to the spliceosome dynamics by facilitating the physical rearrangements of the spliceosome. Here, we report USP15, a deubiquitinating enzyme, as a regulator of protein-protein interactions for the spliceosome ...
Das, Tanuza, Kim, Eunice EunKyeong, Kim, Eunji, Park, Jinyoung, Park, Joon Kyu, Rape, Michael, Song, Eun Joo   +6 more
core   +1 more source

The Regulation of Trace Metal Elements in Cancer Ferroptosis

Advanced Biology, EarlyView.
The induction of ferroptosis inhibits tumor growth, enhances anticancer efficacy, and overcomes drug resistance. Recent evidence shows nonferrous metal elements play a role in ferroptosis. This review focuses on how trace metals regulate ferroptosis processes like iron accumulation, lipid peroxidation, and antioxidant defense.
Xiaoyan Wang, Yuanyuan Xue, Lei Chang, Xuena Zhu, Wenjun Liu, Tingbo Liang   +5 more
wiley   +1 more source

The molecular dissection of TRIM25’s RNA-binding mechanism provides key insights into its antiviral activity

Nature Communications
TRIM25 is an RNA-binding ubiquitin E3 ligase with central but poorly understood roles in the innate immune response to RNA viruses. The link between TRIM25’s RNA binding and its role in innate immunity has not been established.
Lucía Álvarez, Kevin Haubrich, Louisa Iselin, Laurent Gillioz, Vincenzo Ruscica, Karine Lapouge, Sandra Augsten, Ina Huppertz, Nila Roy Choudhury, Bernd Simon, Pawel Masiewicz, Mathilde Lethier, Stephen Cusack, Katrin Rittinger, Frank Gabel, Alexander Leitner, Gracjan Michlewski, Matthias W. Hentze, Frédéric H. T. Allain, Alfredo Castello, Janosch Hennig   +20 more
doaj   +1 more source