Results 121 to 130 of about 1,353,045 (362)

NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes

Nature Communications, 2017
Stress granules (SG) comprise aggregates of cellular messenger ribonucleoproteins (mRNPs) but how they form is unclear. Here, the authors identify NEDD4, an E3 ubiquitin ligase, as regulating the RNA binding protein NANOS2 and turnover of mRNP components,
Zhi Zhou, Hiroshi Kawabe, Atsushi Suzuki, Kaori Shinmyozu, Yumiko Saga   +4 more
doaj   +1 more source

Cloning Full-Length, Cap-Trapper-Selected cDNAs by Using the Single-Strand Linker Ligation Method

BioTechniques, 2001
We have developed the single-strand linker ligation method (SSLLM), which uses DNA ligase to add a dsDNA linker to singlestranded (ss) full-length cDNA. The linkers have random 6-bp (dN6 or dGN5) 3′ overhangs that can ligate to any cDNA sequence, thereby
Y. Shibata, P. Carninci, A. Watahiki, T. Shiraki, H. Konno, M. Muramatsu, Y. Hayashizaki   +6 more
doaj   +1 more source

In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production [PDF]

, 2011
According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity,
Ayaz Ali Khan, Israr Khan, Muhammad Waheed Akktar   +2 more
core   +1 more source

Comparative interactomics analysis of different ALS-associated proteins identifies converging molecular pathways [PDF]

, 2016
Amyotrophic lateral sclerosis (ALS) is a devastating neurological disease with no effective treatment available. An increasing number of genetic causes of ALS are being identified, but how these genetic defects lead to motor neuron degeneration and ...
Anink, Jasper J., Aronica, Eleonora, Blokhuis, Anna M., Bozzoni, Irene, Demmers, Jeroen A. A., Den Hertog, Jeroen, DINI MODIGLIANI, Stefano, Fumoto, Katsumi, Groen, Ewout J. N., Koppers, Max, Pasterkamp, R. Jeroen, Snelting, Anne, Sodaar, Peter, van Den Berg, Leonard H., van Den Heuvel, Dianne M. A., van Diggelen, Femke, Veldink, Jan H., Verheijen, Bert M.   +17 more
core   +3 more sources

T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates [PDF]

European Journal of Biochemistry, 1999
T4 RNA ligase has been shown to synthesize nucleoside and dinucleoside 5′‐polyphosphates by displacement of the AMP from the E‐AMP complex with polyphosphates and nucleoside diphosphates and triphosphates. Displacement of the AMP by tripolyphosphate (P3) was concentration dependent, as measured by SDS/PAGE. When the enzyme was incubated in the presence
Olga Madrid, María A. Günther Sillero, Antonio Sillero, Eva Ana Atencia   +3 more
openaire   +4 more sources

Multifunctional Hydroxyapatite Coated with Gallium Liquid Metal‐Based Silver Nanoparticles for Infection Prevention and Bone Regeneration

Advanced Functional Materials, EarlyView.
A multifunctional hydroxyapatite (HAp) coating integrated with silver‐gallium liquid metal nanoparticles (HAp‐Ag‐GaNPs) exhibits dual antibacterial and osteogenic properties. It effectively inhibits Gram‐positive and Gram‐negative bacteria, including resistant strains, while enhancing bone regeneration.
Ngoc Huu Nguyen, Pengfei Zhang, Fathima Shana Pattar Kadavan, Zhaoning Xu, Tien Thanh Nguyen, Wenshao Li, Manh Tuong Nguyen, Chung Kim Nguyen, Duy Quang Pham, Thi Giang Tuyet Pham, Andrew Hayles, Markos Negash Alemie, Jitraporn Vongsvivut, Vincent Chan, Stephen Peter Kidd, Yunpeng Zhao, Krasimir Vasilev, Vi Khanh Truong   +17 more
wiley   +1 more source

Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: Isolation and characterization of viable and nonviable mutants [PDF]

, 1988
By following a strategy of genetic analysis of poliovirus, we have constructed a synthetic "mutagenesis cartridge" spanning the genome-linked viral protein coding region and flanking cleavage sites in an infectious cDNA clone of the type 1 (Mahoney ...
Dunn, John J., Kuhn, Richard J., Semler, Bert L., Tada, Hiroomi, Wimmer, Eckard, Ypma-Wong, Mary Frances   +5 more
core  

Simple and efficient synthesis of 5′ pre-adenylated DNA using thermostable RNA ligase

Nucleic Acids Research, 2011
We report a simple method of enzymatic synthesis of pre-adenylated DNA linkers/adapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoautotrophicum (MthRnl).
A. Zhelkovsky, L. McReynolds
semanticscholar   +1 more source

Membrane Fusion‐Inspired Nanomaterials: Emerging Strategies for Infectious Disease and Cancer Diagnostics

Advanced Healthcare Materials, EarlyView.
Membrane fusion‐inspired nanomaterials offer transformative potential in diagnostics by mimicking natural fusion processes to achieve highly sensitive and specific detection of disease biomarkers. This review highlights recent advancements in nanomaterial functionalization strategies, signal amplification systems, and stimuli‐responsive fusion designs,
Sojeong Lee, Khulan Nyamzaya, Jueun Han, Yejin Song, Jihee Lee, Jung Hyun Jo, Seungmin Bang, Eun‐Kyung Lim, Seungjoo Haam, Eunjung Kim   +9 more
wiley   +1 more source

Rsp5 promotes Gene Activation mediated by 9aaTAD Transcription Factors Oaf1 and Gal4 [PDF]

, 2009
Nine-amino-acid transactivation domain, 9aaTAD, defines a large superfamily of yeast and mammals transcription factors. The transactivation of the 9aaTAD has been addressed to multiple general co-activators TAF9, MED15, CBP and p300.
Joachim Lipp, Manfred Koranda, Martin Piskacek   +2 more
core   +1 more source