Coevolution of RtcB and Archease created a multiple-turnover RNA ligase. [PDF]
RNA, 2015 RtcB is a noncanonical RNA ligase that joins either 2′,3′-cyclic phosphate or 3′-phosphate termini to 5′-hydroxyl termini. The genes encoding RtcB and Archease constitute a tRNA splicing operon in many organisms.
Desai KK, Beltrame AL, Raines RT.
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Genetic and Functional Analyses of Archaeal ATP-Dependent RNA Ligase in C/D Box sRNA Circularization and Ribosomal RNA Processing [PDF]
Frontiers in Molecular Biosciences, 2022 RNA ligases play important roles in repairing and circularizing RNAs post-transcriptionally. In this study, we generated an allelic knockout of ATP-dependent RNA ligase (Rnl) in the hyperthermophilic archaeon Thermococcus kodakarensis to identify its ...
Yancheng Liu +7 more
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Adenylylation of small RNA sequencing adapters using the TS2126 RNA ligase I. [PDF]
RNA, 2016 Many high-throughput small RNA next-generation sequencing protocols use 5′ preadenylylated DNA oligonucleotide adapters during cDNA library preparation. Preadenylylation of the DNA adapter's 5′ end frees from ATP-dependence the ligation of the adapter to
Lama L, Ryan K.
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tRNA ligase structure reveals kinetic competition between non-conventional mRNA splicing and mRNA decay [PDF]
eLife, 2019 Yeast tRNA ligase (Trl1) is an essential trifunctional enzyme that catalyzes exon-exon ligation during tRNA biogenesis and the non-conventional splicing of HAC1 mRNA during the unfolded protein response (UPR).
Jirka Peschek, Peter Walter
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Plant Methods, 2012 Background Deep sequencing is a powerful tool for novel small RNA discovery. Illumina small RNA sequencing library preparation requires a pre-adenylated 3’ end adapter containing a 5’,5’-adenyl pyrophosphoryl moiety.
Chen Yun-Ru +5 more
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T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis
BMC Biotechnology, 2011 Background T4 RNA ligases 1 and 2 are useful tools for RNA analysis. Their use upstream of RNA analyses such as high-throughput RNA sequencing and microarrays has recently increased their importance.
Zhuang Fanglei +4 more
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Characterization of a novel eukaryal nick-sealing RNA ligase from Naegleria gruberi. [PDF]
RNA, 2015 The proteome of the amoebo-flagellate protozoan Naegleria gruberi is rich in candidate RNA repair enzymes, including 15 putative RNA ligases, one of which, NgrRnl, is a eukaryal homolog of Deinococcus radiodurans RNA ligase, DraRnl.
Unciuleac MC, Shuman S.
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Kinetic mechanism of nick sealing by T4 RNA ligase 2 and effects of 3′-OH base mispairs and damaged base lesions [PDF]
RNA: A publication of the RNA Society, 2013 T4 RNA ligase 2 (Rnl2) seals 3′-OH/5′-PO4 nicks in double-stranded nucleic acids where the broken strand is RNA. This study analyzed the kinetic mechanism and fidelity of nick sealing by Rnl2–AMP.
Mathieu Chauleau, Stewart Shuman
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RtcB, a Novel RNA Ligase, Can Catalyze tRNA Splicing and HAC1 mRNA Splicing in Vivo [PDF]
Journal of Biological Chemistry, 2011 RtcB enzymes are novel RNA ligases that join 2′,3′-cyclic phosphate and 5′-OH ends. The phylogenetic distribution of RtcB points to its candidacy as a tRNA splicing/repair enzyme.
Naoko Tanaka +2 more
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Cleavage of 3'-terminal adenosine by archaeal ATP-dependent RNA ligase. [PDF]
Sci Rep, 2017 Methanothermobacter thermoautotrophicus RNA ligase (MthRnl) catalyzes formation of phosphodiester bonds between the 5′-phosphate and 3′-hydroxyl termini of single-stranded RNAs.
Yoshinari S, Liu Y, Gollnick P, Ho CK.
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