Results 301 to 310 of about 267,980 (331)

Deoxyribozymes with 2‘−5‘ RNA Ligase Activity

Journal of the American Chemical Society, 2003
In vitro selection was used to identify deoxyribozymes that ligate two RNA substrates. In the ligation reaction, a 2'-5' RNA phosphodiester linkage is created from a 2',3'-cyclic phosphate and a 5'-hydroxyl group. The new Mg(2+)-dependent deoxyribozymes provide 50-60% yield of ligated RNA in overnight incubations at pH 7.5 and 37 degrees C, and they ...
Yangming Wang, Kelly A. Hoadley, Imran Rashid, and Amanda C. Wolf, Rebecca L. Coppins, Amber Flynn-Charlebois, Scott K. Silverman, Tracey K. Prior   +7 more
openaire   +3 more sources

The purification of nuclease-free T4-RNA ligase

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
RNA ligase has been highly purified in good yields from bacteriophage T4-infected Escherichia coli by a rapid and reproducible procedure. The enzyme is free of phosphomonoesterase and ribonuclease activities and is therefore suitable for the synthesis of oligoribonucleotides and for the labeling of the 3'-terminus of RNA.
Thomas H. Lubben, Richard I. Gumport, Marie I. Moseman McCoy   +2 more
openaire   +3 more sources

Enzymic oligoribonucleotide synthesis with T4 RNA ligase

Biochemistry, 1978
The substrate specificity of T4 RNA ligase has been examined to determine whether the intermolecular reaction is sufficiently general to realize its potential in the enzymatic synthesis of oligoribonucleotides of defined sequence. Reactions between a variety of acceptor molecules with 3'- and 5'-bydroxyl groups and donor molecules with 3'- and 5 ...
Olke C. Uhlenbeck, Thomas E. England
openaire   +3 more sources

Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase

2014
RNA ligation allows the creation of large RNA molecules from smaller pieces. This can be useful in a number of contexts: to generate molecules that are larger than can be directly synthesized; to incorporate site-specific changes or RNA modifications within a large RNA in order to facilitate functional and structural studies; to isotopically label ...
Stephen D. Rader, Stephen D. Rader, Martha R. Stark   +2 more
openaire   +3 more sources

Donor activation in the T4 RNA ligase reaction

Biochemistry, 1985
T4 RNA ligase catalyzes the adenylation of donor oligonucleotide substrates. These activated intermediates react with an acceptor oligonucleotide which results in phosphodiester bond formation and the concomitant release of AMP. Adenylation of the four common nucleoside 3',5'-bisphosphates as catalyzed by T4 RNA ligase in the absence of an acceptor ...
Erika Graeser, Larry W. McLaughlin, Norbert Piel   +2 more
openaire   +3 more sources

Use of a 2-5A analogue probe for detecting RNA ligase and RNA ligase substrates in mammalian cell extracts

Biochemical and Biophysical Research Communications, 1984
The compound ppp(A2'p)3A3'[32P]pCp is a commercially available radioactive analogue of the 2',5' oligoadenylate series ppp(A2'p)nA, n greater than or equal to 2, commonly referred to as 2-5A. It is used as a probe for measuring concentrations in competition radiobinding and radioimmune assays.
P. Jane Cayley, Walden K. Roberts, Ian M. Kerr   +2 more
openaire   +3 more sources

[2] Using T4 RNA ligase with DNA substrates

1983
Publisher Summary T4 RNA ligase forms a 3´ → 5´ phosphodiester bond between the 3´-hydroxyl and the 5´-phosphate of an oligoribonucleotide with the concomitant cleavage of ATP to AMP and PP i . In addition to the intramolecular circularization activity, the enzyme also joins oligonucleotides in an intermolecular reaction.
Catherine A. Brennan, Alice E. Manthey, Richard I. Gumport   +2 more
openaire   +3 more sources

E3 ligase ligand chemistries: from building blocks to protein degraders

Chemical Society Reviews, 2022
Izidor Sosi, Aleša Bricelj, Christian Steinebach   +2 more
exaly  

On the mechanism of T4 RNA ligase

Journal of the American Chemical Society, 1981
Floyd R. Bryant, Stephen J. Benkovic
openaire   +2 more sources

E3 Ligase Ligands in Successful PROTACs: An Overview of Syntheses and Linker Attachment Points

Frontiers in Chemistry, 2021
Aleša Bricelj, Christian Steinebach, Michael Gütschow   +2 more
exaly