Results 321 to 330 of about 1,353,045 (362)

RNA Ligases

Current Protocols in Molecular Biology, 1991
AbstractT4 RNA ligase, the product of the phage gene 63, is purified from phage‐infected cells. It catalyzes the ATP‐dependent covalent joining of single‐stranded 5'‐phosphoryl termini of DNA or RNA to single‐stranded 3'‐hydroxyl termini of DNA or RNA. This unit describes specific reaction conditions as well as applications such as radioactive labeling
Stanley Tabor, Nicole M. Nichols, Larry A. McReynolds   +2 more
openaire   +3 more sources

Reversal of T4 RNA ligase

Biochemistry, 1982
Unexpected products detected in oligoribonucleotide synthesis reactions catalyzed by T4 RNA ligase are shown to be a result of a partial reversal of the enzyme reaction. A transfer assay for the reversal of the third step in the RNA ligase reaction mechanism and an exchange assay for the reversal of both the second and third steps are described ...
Olke C. Uhlenbeck, Marc S. Krug
openaire   +3 more sources

[3] Joining of RNA molecules with RNA ligase

1983
Publisher Summary T4 RNA ligase catalyzes the ATP-dependent phosphodiester bond formation between a 5´-terminal phosphate (donor) and a 3´-terminal hydroxyl (acceptor). Although the enzyme can produce circular products with substrates of sufficient length that contain the required termini RNA ligase is also a very effective intermolecular joining ...
Paul J. Romaniuk, Olke C. Uhlenbeck
openaire   +3 more sources

Phosphorothioate substrates for T4 RNA ligase

Biochemistry, 1982
Stereochemical details of the T4 RNA ligase reaction mechanism have been delineated by examining the reactivity of phosphorothioate analogues in the adenosine 5'-triphosphate (ATP) dependent and ATP-independent RNA ligase reactions. Only the SP isomer of the diastereomeric dinucleoside thiopyrophosphate, App(s)I, was active as an activated donor ...
Floyd R. Bryant, Stephen J. Benkovic
openaire   +3 more sources

3'-end labeling of RNA with [5'-32P]Cytidine 3',5'-bis(phosphate) and T4 RNA ligase 1.

Cold Spring Harbor Protocols, 2014
This protocol is used to radiolabel the 3' ends of RNAs, either synthesized by in vitro transcription or purified from cells or tissues, by ligation of [5'-(32)P]cytidine 3',5'-bis(phosphate) (pCp).
T. Nilsen
semanticscholar   +1 more source

T4 RNA ligase

Trends in Biochemical Sciences, 1983
Abstract T4 RNA ligase is an indispensible tool for the synthesis and modification of RNA. Despite a clear understanding of its mechanism and its remarkably broad substrate specificity, its role in T4 infection is unknown.
openaire   +2 more sources

Purification of yeast transfer RNA ligase

1990
The splicing of yeast tRNA precursors requires the action of at least two proteins, an endonuclease which cleaves the two splice junctions to release the linear intron and a ligase which joins the resulting tRNA half molecules together. Each of the activities of tRNA ligase can be assayed independent of the overall reaction.
Qi Xu, John Abelson, Eric M. Phizicky, Chris L. Greer   +3 more
openaire   +2 more sources

RNA ligase does the AMP shuffle

Nature Structural & Molecular Biology, 2006
Crystal structures of T4 RNA ligase 2 trapped at different stages of a multistep reaction show how one enzyme active site juggles three phosphoryl transfer reactions to join two RNA strands. The structures also show how the ligase enzyme selectively recognizes the sugar pucker of ribose to achieve catalytic specificity toward RNA-containing substrates.
Tom Ellenberger, John M. Pascal
openaire   +2 more sources

Deoxyribozymes with 2‘−5‘ RNA Ligase Activity

Journal of the American Chemical Society, 2003
In vitro selection was used to identify deoxyribozymes that ligate two RNA substrates. In the ligation reaction, a 2'-5' RNA phosphodiester linkage is created from a 2',3'-cyclic phosphate and a 5'-hydroxyl group. The new Mg(2+)-dependent deoxyribozymes provide 50-60% yield of ligated RNA in overnight incubations at pH 7.5 and 37 degrees C, and they ...
Yangming Wang, Kelly A. Hoadley, Imran Rashid, and Amanda C. Wolf, Rebecca L. Coppins, Amber Flynn-Charlebois, Scott K. Silverman, Tracey K. Prior   +7 more
openaire   +3 more sources

The purification of nuclease-free T4-RNA ligase

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
RNA ligase has been highly purified in good yields from bacteriophage T4-infected Escherichia coli by a rapid and reproducible procedure. The enzyme is free of phosphomonoesterase and ribonuclease activities and is therefore suitable for the synthesis of oligoribonucleotides and for the labeling of the 3'-terminus of RNA.
Thomas H. Lubben, Richard I. Gumport, Marie I. Moseman McCoy   +2 more
openaire   +3 more sources