Results 31 to 40 of about 1,338,157 (346)

RNA ligase RtcB splices 3′-phosphate and 5′-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3′)pp(5′)G intermediates

Proceedings of the National Academy of Sciences of the United States of America, 2012
Anupam K. Chakravarty, Roman I. Subbotin, Brian T. Chait, Stewart Shuman   +3 more
openalex   +2 more sources

Protocol for the generation and purification of high-molecular-weight covalent RNA-DNA hybrids with T4 RNA ligase [PDF]

STAR Protoc
Laguía O, Bosso G, Martínez-Torrecuadrada J, Míguez-Amil S, Fernández-Leiro R, Blasco M.   +5 more
europepmc   +2 more sources

The rna-binding ubiquitin ligase mex3a affects glioblastoma tumorigenesis by inducing ubiquitylation and degradation of rig-i [PDF]

, 2020
Glioblastoma multiforme (GB) is the most malignant primary brain tumor in humans, with an overall survival of approximatively 15 months. The molecular heterogeneity of GB, as well as its rapid progression, invasiveness and the occurrence of drug ...
Basili, I., Bufalieri, F., Caimano, M., Canettieri, G., D'angelo, L., Di Marcotullio, L., Infante, P., Loricchio, E., Paglia, F., Petroni, M., Sampirisi, L., Santoro, A., Severini, L. L.   +12 more
core   +1 more source

Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit

Biology, 2023
The class I ligase ribozyme consists of 121 nucleotides and shows a high catalytic rate comparable to that found in natural proteinaceous polymerases. In this study, we aimed to identify the smaller active unit of the class I ligase ribozyme comprising ...
Miho Kasuga, Hiromi Mutsuro-Aoki, Tadashi Ando, Koji Tamura   +3 more
doaj   +1 more source

Ribozyme activity modulates the physical properties of RNA–peptide coacervates

eLife, 2023
Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology.
Kristian Kyle Le Vay, Elia Salibi, Basusree Ghosh, TY Dora Tang, Hannes Mutschler   +4 more
doaj   +1 more source

Accurate RNA 3D structure prediction using a language model-based deep learning approach [PDF]

Nature Methods 2024, 2022
Accurate prediction of RNA three-dimensional (3D) structure remains an unsolved challenge. Determining RNA 3D structures is crucial for understanding their functions and informing RNA-targeting drug development and synthetic biology design. The structural flexibility of RNA, which leads to scarcity of experimentally determined data, complicates ...
arxiv   +1 more source

Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]

, 2006
The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Amitsur, Arabshahi, Bebenek, Begley, Bellacosa, Blondal, Blondal, Cherepanov, Cherepanov, Clepet, Della, Desmond R. Bullard, Doherty, Engler, Fareed, Higgins, Ho, Ho, Kaufmann, Keppetipola, Kornberg, Kuhn, Lavesa-Curto, Lehman, Markham, Martins, Martins, Miller, Moore, Nandakumar, Nandakumar, Nandakumar, Nick McElhinny, Omari, Pascal, Pitcher, Pitcher, Richard P. Bowater, Rossi, Ruiz, Runnels, Rydberg, Sambrook, Sekiguchi, Shuman, Shuman, Snopek, Sriskanda, Stuart, Timson, Tomkinson, Wang, Wang, Weiss, Wilkinson, Wilkinson, Wilkinson, Wood, Wood, Worthey, Yin, Yin   +61 more
core   +4 more sources

Trim25 is an RNA-specific activator of Lin28a/TuT4-mediated uridylation [PDF]

, 2014
SummaryRNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4 ...
Ascano, Baltz, Castello, Chang, Cho, Choudhury, Choudhury, Gack, Glisovic, Guil, Hafner, Hagan, Havens, Hentze, Heo, Heo, Inn, Kwon, Lee, Lehrbach, Lightfoot, Loughlin, Michlewski, Michlewski, Michlewski, Michlewski, Milek, Nam, Nowak, Nowak, Pandit, Piskounova, Ray, Rybak, Shyh-Chang, Shyh-Chang, Singh, Thornton, Towbin, Urbach, Ustianenko, Van Wynsberghe, Versteeg, Viswanathan, Wilbert, Yang   +45 more
core   +6 more sources

TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Vesicular Stomatitis Virus Infection

Viruses, 2020
Vesicular stomatitis virus (VSV) is a zoonotic, negative-stranded RNA virus of the family Rhabdoviridae. The nucleoprotein (N) of VSV protects the viral genomic RNA and plays an essential role in viral transcription and replication, which makes the ...
Girish Patil, Lingling Xu, Yakun Wu, Kun Song, Wenzhuo Hao, Fang Hua, Lingyan Wang, Shitao Li   +7 more
doaj   +1 more source

The Role of ZAP and TRIM25 RNA Binding in Restricting Viral Translation

Frontiers in Cellular and Infection Microbiology, 2022
The innate immune response controls the acute phase of virus infections; critical to this response is the induction of type I interferon (IFN) and resultant IFN-stimulated genes to establish an antiviral environment.
Emily Yang, Emily Yang, LeAnn P. Nguyen, LeAnn P. Nguyen, Carlyn A. Wisherop, Ryan L. Kan, Ryan L. Kan, Melody M.H. Li, Melody M.H. Li, Melody M.H. Li   +9 more
doaj   +1 more source