Results 41 to 50 of about 1,338,157 (346)
A streamlined protocol for the detection of mRNA–sRNA interactions using AMT-crosslinking in vitro
BioTechniques, 2019 Until recently, RNA–RNA interactions were mainly identified by crosslinking RNAs with interacting proteins, RNA proximity ligation and deep sequencing. Recently, AMT-based direct RNA crosslinking was established.
Rebecca Kirsch +6 more
doaj +1 more source
Identification of novel components of Trypanosoma brucei editosomes [PDF]
, 2003 The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome proteins in Trypanosoma brucei.
Carmean, Nicole +6 more
core +2 more sources
Tumor suppressor mediated ubiquitylation of hnRNPK is a barrier to oncogenic translation
Nature Communications, 2022 Cytoplasmic accumulation of RNA binding protein, hnRNPK in cancer cells is associated with poor prognosis. Here the authors show that SCFFbxo4 E3 ubiquitin ligase-mediated polyubiquitylation of hnRNPK restricts c-Myc translation and limits cancer ...
Bartosz Mucha +23 more
doaj +1 more source
Isolation of novel ribozymes that ligate AMP-activated RNA substrates [PDF]
, 1997 Background: The protein enzymes RNA ligase and DNA ligase catalyze the ligation of nucleic acids via an adenosine-5′-5′-pyrophosphate ‘capped’ RNA or DNA intermediate. The activation of nucleic acid substrates by adenosine 5′-monophosphate (AMP) may be a
Hager, Alicia J., Szostak, Jack W.
core +1 more source
Scaling properties of RNA as a randomly branching polymer [PDF]
J. Chem. Phys. 158, 234901 (2023), 2023 Formation of base pairs between the nucleotides of an RNA sequence gives rise to a complex and often highly branched RNA structure. While numerous studies have demonstrated the functional importance of the high degree of RNA branching -- for instance, for its spatial compactness or interaction with other biological macromolecules -- RNA branching ...
arxiv +1 more source
An improved protocol for small RNA library construction using High Definition adapters [PDF]
, 2015 Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin +28 more
core +1 more source
Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]
, 2013 Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward +2 more
core +1 more source
Nucleic Acids Research, 2016 An ATP-dependent RNA ligase from Methanobacterium thermoautotrophicum (MthRnl) catalyzes intramolecular ligation of single-stranded RNA to form a closed circular RNA via covalent ligase-AMP and RNA-adenylylate intermediate.
Huiqiong Gu +6 more
semanticscholar +1 more source
Reconciling Ligase Ribozyme Activity with Fatty Acid Vesicle Stability
Life, 2014 The “RNA world” and the “Lipid world” theories for the origin of cellular life are often considered incompatible due to the differences in the environmental conditions at which they can emerge.
Fabrizio Anella, Christophe Danelon
doaj +1 more source
The subnuclear localization of tRNA ligase in yeast [PDF]
, 1987 Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core +1 more source