Results 41 to 50 of about 1,353,045 (362)

Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1 [PDF]

Nucleic Acids Research, 2003
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of genes with potential use in
Sigridur Hjorleifsdottir, Gudmundur O. Hreggvidsson, Olafur F. Fridjonsson, Albert Vernon Smith, Jakob K. Kristjansson, Arnthor Aevarsson, Anna Gudny Hermannsdottir, Thorarinn Blondal, Sigurlaug Skirnisdottir   +8 more
openaire   +3 more sources

Ribozyme activity modulates the physical properties of RNA–peptide coacervates

eLife, 2023
Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology.
Kristian Kyle Le Vay, Elia Salibi, Basusree Ghosh, TY Dora Tang, Hannes Mutschler   +4 more
doaj   +1 more source

Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit

Biology, 2023
The class I ligase ribozyme consists of 121 nucleotides and shows a high catalytic rate comparable to that found in natural proteinaceous polymerases. In this study, we aimed to identify the smaller active unit of the class I ligase ribozyme comprising ...
Miho Kasuga, Hiromi Mutsuro-Aoki, Tadashi Ando, Koji Tamura   +3 more
doaj   +1 more source

Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]

, 2006
The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Amitsur, Arabshahi, Bebenek, Begley, Bellacosa, Blondal, Blondal, Cherepanov, Cherepanov, Clepet, Della, Desmond R. Bullard, Doherty, Engler, Fareed, Higgins, Ho, Ho, Kaufmann, Keppetipola, Kornberg, Kuhn, Lavesa-Curto, Lehman, Markham, Martins, Martins, Miller, Moore, Nandakumar, Nandakumar, Nandakumar, Nick McElhinny, Omari, Pascal, Pitcher, Pitcher, Richard P. Bowater, Rossi, Ruiz, Runnels, Rydberg, Sambrook, Sekiguchi, Shuman, Shuman, Snopek, Sriskanda, Stuart, Timson, Tomkinson, Wang, Wang, Weiss, Wilkinson, Wilkinson, Wilkinson, Wood, Wood, Worthey, Yin, Yin   +61 more
core   +4 more sources

TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Vesicular Stomatitis Virus Infection

Viruses, 2020
Vesicular stomatitis virus (VSV) is a zoonotic, negative-stranded RNA virus of the family Rhabdoviridae. The nucleoprotein (N) of VSV protects the viral genomic RNA and plays an essential role in viral transcription and replication, which makes the ...
Girish Patil, Lingling Xu, Yakun Wu, Kun Song, Wenzhuo Hao, Fang Hua, Lingyan Wang, Shitao Li   +7 more
doaj   +1 more source

A conformational RNA zipper promotes intron ejection during non-conventional XBP1 mRNA splicing. [PDF]

, 2015
The kinase/endonuclease IRE1 is the most conserved signal transducer of the unfolded protein response (UPR), an intracellular signaling network that monitors and regulates the protein folding capacity of the endoplasmic reticulum (ER).
Acosta-Alvear, Diego, Mendez, Aaron S, Peschek, Jirka, Walter, Peter   +3 more
core   +1 more source

A streamlined protocol for the detection of mRNA–sRNA interactions using AMT-crosslinking in vitro

BioTechniques, 2019
Until recently, RNA–RNA interactions were mainly identified by crosslinking RNAs with interacting proteins, RNA proximity ligation and deep sequencing. Recently, AMT-based direct RNA crosslinking was established.
Rebecca Kirsch, V Janett Olzog, Sonja Bonin, Christina E Weinberg, Heike Betat, Peter F Stadler, Mario Mörl   +6 more
doaj   +1 more source

The Role of ZAP and TRIM25 RNA Binding in Restricting Viral Translation

Frontiers in Cellular and Infection Microbiology, 2022
The innate immune response controls the acute phase of virus infections; critical to this response is the induction of type I interferon (IFN) and resultant IFN-stimulated genes to establish an antiviral environment.
Emily Yang, Emily Yang, LeAnn P. Nguyen, LeAnn P. Nguyen, Carlyn A. Wisherop, Ryan L. Kan, Ryan L. Kan, Melody M.H. Li, Melody M.H. Li, Melody M.H. Li   +9 more
doaj   +1 more source

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]

, 2013
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward, Brissett, Nigel C, Doherty, Aidan J   +2 more
core   +1 more source

The subnuclear localization of tRNA ligase in yeast [PDF]

, 1987
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core   +1 more source