Results 91 to 100 of about 2,625,316 (382)

P‐glycoprotein modulates the fluidity gradient of the plasma membrane of multidrug resistant CHO cells

FEBS Letters, EarlyView.
To explore the impact of the overexpression of the multidrug‐transporter P‐glycoprotein (ABCB1) on membrane fluidity, we compared the transversal gradient of mobility and microviscosity in plasma membranes of drug‐sensitive Chinese hamster ovary cells (AuxB1) and their multidrug‐resistant derivatives (B30) using the fluorescent n‐(9‐anthroyloxy) fatty ...
Roger Busche, John R. Riordan, Burkhard Tümmler   +2 more
wiley   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

T7 RNA Polymerase

Uirusu, 2001
The T7 bacteriophage encodes a single-subunit RNA polymerase of 99 kDa that transcribes the phage's genes during its lytic growth in Escherichia coli. The T7 RNA polymerase is structurally similar to DNA polymerases, reverse transcriptases, and RNA-directed RNA polymerases, and these structural similarities define a polymerase superfamily that includes
openaire   +5 more sources

Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA. [PDF]

, 1985
The bacteriophage SP6 promoter and RNA polymerase were used to synthesize sense and antisense RNAs coding for the enzymes thymidine kinase (TK) and chloramphenicol acetyl transferase (CAT).
HARLAND, Richard M., Weintraub, H
core   +2 more sources

Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex

Nature Chemical Biology, 2017
Quantitative measurement of transcription rates in live cells is important for revealing mechanisms of transcriptional regulation. This is particularly challenging for measuring the activity of RNA polymerase III (Pol III), which transcribes growth ...
Wenjiao Song, Grigory S. Filonov, Hyaeyeong Kim, Markus Hirsch, Xing Li, Jared D Moon, S. Jaffrey   +6 more
semanticscholar   +1 more source

Clinical applications of next‐generation sequencing‐based ctDNA analyses in breast cancer: defining treatment targets and dynamic changes during disease progression

Molecular Oncology, EarlyView.
Circulating tumor DNA (ctDNA) offers a possibility for different applications in early and late stage breast cancer management. In early breast cancer tumor informed approaches are increasingly used for detecting molecular residual disease (MRD) and early recurrence. In advanced stage, ctDNA provides a possibility for monitoring disease progression and
Eva Valentina Klocker, Samantha Hasenleithner, Rupert Bartsch, Simon P. Gampenrieder, Daniel Egle, Christian F. Singer, Gabriel Rinnerthaler, Michael Hubalek, Katja Schmitz, Zsuzsanna Bago‐Horvath, Andreas Petzer, Sonja Heibl, Ellen Heitzer, Marija Balic, Michael Gnant   +14 more
wiley   +1 more source

Transcription factors modulate RNA polymerase conformational equilibrium

Nature Communications, 2022
Pausing of RNA polymerase (RNAP) and transcription is regulated by the NusA and NusG transcription factors in bacteria. Here the authors provide structural evidence for how they interact with RNAP to carry out their pausing roles and also reveal ...
Chengjin Zhu, Xieyang Guo, Philippe Dumas, Maria Takacs, Mo’men Abdelkareem, Arnaud Vanden Broeck, Charlotte Saint-André, Gabor Papai, Corinne Crucifix, Julio Ortiz, Albert Weixlbaumer   +10 more
doaj   +1 more source

Properties of RNA Polymerases from B16 Melanoma [PDF]

, 1973
Some of the properties of RNA polymerases from melanotic and amelanotic B16 melanomas have been studied. Chromatin-bound RNA polymerase is activated by Mg++ more the Mn++. Addition of ammonium sulfate was found to activate the enzyme.
Haberman, H.F., Menon, I.A.
core   +1 more source

Circulating tumor cells: advancing personalized therapy in small cell lung cancer patients

Molecular Oncology, EarlyView.
Small cell lung cancer (SCLC) is an aggressive form of lung cancer that spreads rapidly to secondary sites such as the brain and liver. Cancer cells circulating in the blood, “circulating tumor cells” (CTCs), have demonstrated prognostic value in SCLC, and evaluating biomarkers on CTCs could guide treatment decisions such as for PARP inhibitors ...
Prajwol Shrestha, Steven Kao, Veronica K. Cheung, Wendy A. Cooper, Nico van Zandwijk, John E. J. Rasko, Dannel Yeo   +6 more
wiley   +1 more source

Evolutionary connection between the catalytic subunits of DNA-dependent RNA polymerases and eukaryotic RNA-dependent RNA polymerases and the origin of RNA polymerases [PDF]

BMC Structural Biology, 2003
The eukaryotic RNA-dependent RNA polymerase (RDRP) is involved in the amplification of regulatory microRNAs during post-transcriptional gene silencing. This enzyme is highly conserved in most eukaryotes but is missing in archaea and bacteria. No evolutionary relationship between RDRP and other polymerases has been reported so far, hence the origin of ...
Lakshminarayan M. Iyer, Eugene V. Koonin, L. Aravind   +2 more
openaire   +2 more sources