Results 21 to 30 of about 829,079 (264)
Biomolecular Concepts, 2016 The bacterial RNA polymerase (RNAP) holoenzyme is a multisubunit core enzyme associated with a σ factor that is required for promoter-specific transcription initiation. Besides a primary σ responsible for most of the gene expression during active growth,
Cavaliere Paola, Norel Françoise
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Amplification of RNA by an RNA polymerase ribozyme [PDF]
Proceedings of the National Academy of Sciences, 2016 Significance Darwinian life requires the ability to replicate genotypes and express phenotypes. Although all extant life relies on protein enzymes to accomplish these tasks, life in the ancestral RNA world would have used only RNA enzymes. Here, we report the in vitro evolution of an improved RNA polymerase ribozyme that is able to synthesize
David P, Horning, Gerald F, Joyce
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Origin and Evolution of RNA-Dependent RNA Polymerase
Frontiers in Genetics, 2017 RNA-dependent RNA polymerases (RdRp) are very ancient enzymes and are essential for all viruses with RNA genomes. We reconstruct the origin and evolution of this polymerase since the initial stages of the origin of life. The origin of the RdRp was traced
Savio T. de Farias +3 more
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N4 RNA Polymerase II, a Heterodimeric RNA Polymerase with Homology to the Single-Subunit Family of RNA Polymerases [PDF]
Journal of Bacteriology, 2002 ABSTRACTBacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3.
S H, Willis +3 more
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Frontiers in Bioscience-LandmarkAccurate gene expression is fundamental for sustaining life, enabling adaptive responses to routine tasks and management of urgent cellular environments.
Jihye Ryu, Chaeyoung Lee
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In vitro Assessment of RNA Polymerase I Activity
Bio-Protocol, 2017 In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs ...
Marzia Govoni
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eLife, 2015 Errors during transcription may play an important role in determining cellular phenotypes: the RNA polymerase error rate is >4 orders of magnitude higher than that of DNA polymerase and errors are amplified >1000-fold due to translation.
Lucas B Carey
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Nature Communications, 2023 Hantaviruses are causing life-threatening zoonotic infections in humans. Their tripartite negative-stranded RNA genome is replicated by the multi-functional viral RNA-dependent RNA-polymerase.
Quentin Durieux Trouilleton +4 more
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RNA-dependent RNA Polymerase Assay for Hepatitis E Virus
Bio-Protocol, 2017 RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome.
Vidya Nair +3 more
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SARS-CoV-2 RNA Dependent RNA polymerase (RdRp) – A drug repurposing study
Heliyon, 2020 The outbreak of SARS-CoV-2 in December 2019 in China subsequently lead to a pandemic. Lack of vaccine and specific anti-viral drugs started a global health disaster. For a sustained control and protection, development of potential anti-viral drugs is one
Jamshaid Ahmad +4 more
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