Results 51 to 60 of about 2,730,472 (338)
eLife, 2015 Errors during transcription may play an important role in determining cellular phenotypes: the RNA polymerase error rate is >4 orders of magnitude higher than that of DNA polymerase and errors are amplified >1000-fold due to translation.
Lucas B Carey
doaj +1 more source
Rescue of Synthetic Genomic RNA Analogs of Rabies Virus by Plasmid-Encoded Proteins [PDF]
, 1994 Proteins eolirely expressed from cDNA wen used to rescue synthetic RNA genome analogs into infectious defective particles or rabies virus (RV). Synthetic negative-stranded RNAs coßtalning 3' · and S'-terminal RV sequences and tnlßscriptional ...
Conzelmann, Karl-Klaus, Schnell, M.-J.
core +3 more sources
Activation of σ28-dependent transcription inEscherichia coliby the cyclic AMP receptor protein requires an unusual promoter organization [PDF]
, 2010 The Escherichia coli aer regulatory region contains a single promoter that is recognized by RNA polymerase containing the flagellar sigma factor, σ28.
Busby, Stephen J. W. +3 more
core +2 more sources
Rad27/FEN1 prevents accumulation of Okazaki fragments and ribosomal DNA copy number changes
FEBS Letters, EarlyView.The budding yeast Rad27 is a structure‐specific endonuclease. Here, the authors reveal that Rad27 is crucial for maintaining the stability of the ribosomal RNA gene (rDNA) region. Rad27 deficiency leads to the accumulation of Okazaki fragments and changes in rDNA copy number.
Tsugumi Yamaji +3 more
wiley +1 more source
RNA-dependent RNA Polymerase Assay for Hepatitis E Virus
Bio-Protocol, 2017 RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome.
Vidya Nair +3 more
doaj +1 more source
Structure of human RNA polymerase III
Nature Communications, 2020 The eukaryotic RNA Polymerase III transcribes tRNAs, some ribosomal and spliceosomal RNAs. Here, the authors resolve a cryo-EM structure of human RNA Polymerase III in its apo form and complemented it with crystal structures and SAXS analysis of RPC5 ...
Ewan Phillip Ramsay +10 more
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Accurate RT-qPCR gene expression analysis on cell culture lysates [PDF]
, 2012 Gene expression quantification on cultured cells using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) typically involves an RNA purification step that limits sample processing throughput and precludes parallel analysis of ...
Mestdagh, Pieter +2 more
core +2 more sources
FEBS Letters, EarlyView.This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley +1 more source
SARS-CoV-2 RNA Dependent RNA polymerase (RdRp) – A drug repurposing study
Heliyon, 2020 The outbreak of SARS-CoV-2 in December 2019 in China subsequently lead to a pandemic. Lack of vaccine and specific anti-viral drugs started a global health disaster. For a sustained control and protection, development of potential anti-viral drugs is one
Jamshaid Ahmad +4 more
doaj +1 more source
Analysis of Template Variations on RNA Synthesis by Respiratory Syncytial Virus Polymerase
Viruses, 2022 Respiratory syncytial virus (RSV) is a significant threat to infants and elderly individuals globally. Currently, there are no effective therapies or treatments for RSV infection because of an insufficient understanding of the RSV viral machinery.
Dongdong Cao +8 more
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