Molecular insight into RNA polymerase I promoter recognition and promoter melting
Nature Communications, 2019 RNA polymerase I (Pol I) catalyses the transcription of pre-ribosomal RNA and for transcription initiation Pol I assembles with core factor and Rrn3 on the rDNA core promoter.
Yashar Sadian +6 more
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The conserved RNA-binding protein Seb1 promotes cotranscriptional ribosomal RNA processing by controlling RNA polymerase I progression [PDF]
Nature Communications, 2023 Transcription by RNA polymerase I (RNAPI) represents most of the transcriptional activity in eukaryotic cells and is associated with the production of mature ribosomal RNA (rRNA).
Maxime Duval +5 more
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The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription
eLife, 2017 Cell growth requires synthesis of ribosomal RNA by RNA polymerase I (Pol I). Binding of initiation factor Rrn3 activates Pol I, fostering recruitment to ribosomal DNA promoters.
Eva Torreira +9 more
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TblncRNA-23, a long non-coding RNA transcribed by RNA polymerase I, regulates developmental changes in Trypanosoma brucei [PDF]
Nature CommunicationsThe protozoan parasite Trypanosoma brucei undergoes a complex life cycle, moving between its mammalian host and the blood-feeding tsetse fly vector. The two major surface proteins expressed by procyclic forms in the insect midgut, EP and GPEET procyclin,
Beathrice Galili-Kostin +10 more
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Structure of the initiation-competent RNA polymerase I and its implication for transcription
Nature Communications, 2016 Eukaryotic RNA polymerase I (Pol I) is responsible for the transcription of rRNA genes. Here the authors determine the cryo-EM structure of the Pol I-Rrn3 complex, providing insight into how Rrn3 stabilizes the monomeric initiation competent Pol I to ...
Michael Pilsl +8 more
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Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus [PDF]
Poultry ScienceReverse genetics (RG) systems are extensively utilized to investigate the characteristics of influenza viruses and develop vaccines, predominantly relying on human RNA polymerase I (pol I). However, the efficiency of RG systems for avian-origin influenza
Yunyueng Jang +7 more
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Cryo-EM uncovers a sequential mechanism for RNA polymerase I pausing and stalling at abasic DNA lesions [PDF]
Nature CommunicationsDuring synthesis of the ribosomal RNA precursor, RNA polymerase I (Pol I) monitors DNA integrity but its response to DNA damage remains poorly studied.
Alicia Santos-Aledo +8 more
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Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis. [PDF]
PLoS Genetics, 2015 Ribosomal RNA synthesis is controlled by nutrient signaling through the mechanistic target of rapamycin complex 1 (mTORC1) pathway. mTORC1 regulates ribosomal RNA expression by affecting RNA Polymerase I (Pol I)-dependent transcription of the ribosomal ...
R Nicholas Laribee +3 more
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Regulation of Eukaryotic RNAPs Activities by Phosphorylation
Frontiers in Molecular Biosciences, 2021 Evolutionarily conserved kinases and phosphatases regulate RNA polymerase II (RNAPII) transcript synthesis by modifying the phosphorylation status of the carboxyl-terminal domain (CTD) of Rpb1, the largest subunit of RNAPII.
Araceli González-Jiménez +5 more
doaj +1 more source
Direct Characterization of Transcription Elongation by RNA Polymerase I. [PDF]
PLoS ONE, 2016 RNA polymerase I (Pol I) transcribes ribosomal DNA and is responsible for more than 60% of transcription in a growing cell. Despite this fundamental role that directly impacts cell growth and proliferation, the kinetics of transcription by Pol I are ...
Suleyman Ucuncuoglu +5 more
doaj +1 more source