Results 11 to 20 of about 950,582 (330)
In vitro Assessment of RNA Polymerase I Activity
Bio-Protocol, 2017 In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs ...
Marzia Govoni
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Regulation of RNA Polymerase I Stability and Function. [PDF]
Cancers (Basel), 2022 RNA polymerase I is a highly processive enzyme with fast initiation and elongation rates. The structure of Pol I, with its in-built RNA cleavage ability and incorporation of subunits homologous to transcription factors, enables it to quickly and efficiently synthesize the enormous amount of rRNA required for ribosome biogenesis.
Pitts S, Laiho M.
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Functional Architecture of RNA Polymerase I [PDF]
Cell, 2007 Synthesis of ribosomal RNA (rRNA) by RNA polymerase (Pol) I is the first step in ribosome biogenesis and a regulatory switch in eukaryotic cell growth. Here we report the 12 A cryo-electron microscopic structure for the complete 14-subunit yeast Pol I, a homology model for the core enzyme, and the crystal structure of the subcomplex A14/43.
Kuhn, C. +9 more
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Regulation of Eukaryotic RNAPs Activities by Phosphorylation
Frontiers in Molecular Biosciences, 2021 Evolutionarily conserved kinases and phosphatases regulate RNA polymerase II (RNAPII) transcript synthesis by modifying the phosphorylation status of the carboxyl-terminal domain (CTD) of Rpb1, the largest subunit of RNAPII.
Araceli González-Jiménez +5 more
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Direct Characterization of Transcription Elongation by RNA Polymerase I. [PDF]
PLoS ONE, 2016 RNA polymerase I (Pol I) transcribes ribosomal DNA and is responsible for more than 60% of transcription in a growing cell. Despite this fundamental role that directly impacts cell growth and proliferation, the kinetics of transcription by Pol I are ...
Suleyman Ucuncuoglu +5 more
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The RNA polymerase I transcription machinery [PDF]
Biochemical Society Symposia, 2006 The rRNAs constitute the catalytic and structural components of the ribosome, the protein synthesis machinery of cells. The level of rRNA synthesis, mediated by Pol I (RNA polymerase I), therefore has a major impact on the life and destiny of a cell.
Russell, Jackie +1 more
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Solving the RNA polymerase I structural puzzle [PDF]
Acta Crystallographica Section D Biological Crystallography, 2014 Knowing the structure of multi-subunit complexes is critical to understand basic cellular functions. However, when crystals of these complexes can be obtained they rarely diffract beyond 3 Å resolution, which complicates X-ray structure determination and refinement.
Moreno-Morcillo, Maria +8 more
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RNA polymerase I structure and transcription regulation [PDF]
Nature, 2013 Transcription of ribosomal RNA by RNA polymerase (Pol) I initiates ribosome biogenesis and regulates eukaryotic cell growth. The crystal structure of Pol I from the yeast Saccharomyces cerevisiae at 2.8 Å resolution reveals all 14 subunits of the 590-kilodalton enzyme, and shows differences to Pol II.
Engel, C. +4 more
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RNA polymerase I, bending the rules? [PDF]
The EMBO Journal, 2017 Transcription initiation is one of the key regulatory steps in expressing the genetic information encoded in the DNA. Mechanisms of RNA Pol II transcription have been extensively studied, whereas the structural basis of RNA Pol I and III transcription is still poorly defined.
Laura Jochem +2 more
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Molecular Evolution of RNA-Dependent RNA Polymerase Region in Norovirus Genogroup I
Viruses, 2023 Norovirus is the leading viral agent of gastroenteritis in humans. RNA-dependent RNA polymerase (RdRp) is essential in the replication of norovirus RNA. Here, we present a comprehensive evolutionary analysis of the norovirus GI RdRp gene.
Nan Zhou +4 more
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