Molecular Structures of Transcribing RNA Polymerase I [PDF]
Molecular Cell, 2016 RNA polymerase I (Pol I) is a 14-subunit enzyme that solely synthesizes pre-ribosomal RNA. Recently, the crystal structure of apo Pol I gave unprecedented insight into its molecular architecture. Here, we present three cryo-EM structures of elongating Pol I, two at 4.0 Å and one at 4.6 Å resolution, and a Pol I open complex at 3.8 Å resolution.
Lucas Tafur +7 more
openaire +4 more sources
RNA polymerase I–Rrn3 complex at 4.8 Å resolution
Nature Communications, 2016 RNA polymerase I is the central enzyme that synthesizes ribosomal RNA in eukaryotic cells, and its regulation underlies cell growth. Here the authors present a high-resolution cryo-EM structure of the Pol I-Rrn3 complex that explains how Rrn3 ...
Christoph Engel +2 more
doaj +2 more sources
Targeting the RNA Polymerase I Transcription for Cancer Therapy Comes of Age
Cells, 2020 Transcription of the ribosomal RNA genes (rDNA) that encode the three largest ribosomal RNAs (rRNA), is mediated by RNA Polymerase I (Pol I) and is a key regulatory step for ribosomal biogenesis.
Rita Ferreira +4 more
doaj +2 more sources
Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
eLife, 2017 Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Saccharomyces cerevisiae Pol I-CF-DNA to 3.8 Å
Yan Han +6 more
doaj +2 more sources
CTCF regulates the local epigenetic state of ribosomal DNA repeats [PDF]
Epigenetics & Chromatin, 2010 Background: CCCTC binding factor (CTCF) is a highly conserved zinc finger protein, which is involved in chromatin organization, local histone modifications, and RNA polymerase II-mediated gene transcription.
Delgado, M Dolores +14 more
core +7 more sources
RNA polymerase I structure and transcription regulation [PDF]
Nature, 2013 Transcription of ribosomal RNA by RNA polymerase (Pol) I initiates ribosome biogenesis and regulates eukaryotic cell growth. The crystal structure of Pol I from the yeast Saccharomyces cerevisiae at 2.8 Å resolution reveals all 14 subunits of the 590-kilodalton enzyme, and shows differences to Pol II.
Engel, C. +4 more
openaire +7 more sources
Solving the RNA polymerase I structural puzzle [PDF]
Acta Crystallographica Section D Biological Crystallography, 2014 Knowing the structure of multi-subunit complexes is critical to understand basic cellular functions. However, when crystals of these complexes can be obtained they rarely diffract beyond 3 Å resolution, which complicates X-ray structure determination and refinement.
Moreno-Morcillo, Maria +8 more
openaire +7 more sources
The conserved RNA-binding protein Seb1 promotes cotranscriptional ribosomal RNA processing by controlling RNA polymerase I progression. [PDF]
Nat Commun, 2023 Transcription by RNA polymerase I (RNAPI) represents most of the transcriptional activity in eukaryotic cells and is associated with the production of mature ribosomal RNA (rRNA).
Duval M +5 more
europepmc +2 more sources
Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast [PDF]
PLoS Genetics, 2021 The adjustment of transcription and translation rates to the changing needs of cells is of utmost importance for their fitness and survival. We have previously shown that the global transcription rate for RNA polymerase II in budding yeast Saccharomyces ...
Barba-Aliaga, Marina +5 more
core +3 more sources
Molecular insight into RNA polymerase I promoter recognition and promoter melting
Nature Communications, 2019 RNA polymerase I (Pol I) catalyses the transcription of pre-ribosomal RNA and for transcription initiation Pol I assembles with core factor and Rrn3 on the rDNA core promoter.
Yashar Sadian +6 more
doaj +2 more sources