Results 21 to 30 of about 807,500 (308)
RNA polymerase I, bending the rules? [PDF]
The EMBO Journal, 2017 Transcription initiation is one of the key regulatory steps in expressing the genetic information encoded in the DNA. Mechanisms of RNA Pol II transcription have been extensively studied, whereas the structural basis of RNA Pol I and III transcription is still poorly defined.
Laura Jochem +2 more
openaire +4 more sources
Molecular Evolution of RNA-Dependent RNA Polymerase Region in Norovirus Genogroup I
Viruses, 2023 Norovirus is the leading viral agent of gastroenteritis in humans. RNA-dependent RNA polymerase (RdRp) is essential in the replication of norovirus RNA. Here, we present a comprehensive evolutionary analysis of the norovirus GI RdRp gene.
Nan Zhou +4 more
doaj +1 more source
Density- and elongation speed-dependent error correction in RNA polymerization [PDF]
, 2021 Backtracking of RNA polymerase (RNAP) is an important pausing mechanism during DNA transcription that is part of the error correction process that enhances transcription fidelity. We model the backtracking mechanism of RNA polymerase, which usually happens when the polymerase tries to incorporate a mismatched nucleotide triphosphate.
arxiv +1 more source
An RNA polymerase I enhancer in Saccharomyces cerevisiae. [PDF]
Molecular and Cellular Biology, 1986 By the use of an artificial gene coding for rRNA (rDNA gene), we found that transcription of the major precursor rRNA in Saccharomyces cerevisiae cells is stimulated 15-fold by a positive control element located 2 kilobases upstream of the transcription initiation site.
Elaine A. Elion, Jonathan R. Warner
openaire +3 more sources
Autophagy regulates rRNA synthesis
Nucleus, 2022 Autophagy has emerged as a key regulator of cell metabolism. Recently, we have demonstrated that autophagy is involved in RNA metabolism by regulating ribosomal RNA (rRNA) synthesis.
Yinfeng Xu, Wei Wan
doaj +1 more source
Molecular Structures of Transcribing RNA Polymerase I [PDF]
Molecular Cell, 2016 RNA polymerase I (Pol I) is a 14-subunit enzyme that solely synthesizes pre-ribosomal RNA. Recently, the crystal structure of apo Pol I gave unprecedented insight into its molecular architecture. Here, we present three cryo-EM structures of elongating Pol I, two at 4.0 Å and one at 4.6 Å resolution, and a Pol I open complex at 3.8 Å resolution.
Lucas Tafur +7 more
openaire +3 more sources
eLife, 2015 Errors during transcription may play an important role in determining cellular phenotypes: the RNA polymerase error rate is >4 orders of magnitude higher than that of DNA polymerase and errors are amplified >1000-fold due to translation.
Lucas B Carey
doaj +1 more source
Identification of the RNA polymerase I-RNA interactome
Nucleic Acids Research, 2018 Ribosome biogenesis is a complex process orchestrated by a host of ribosome assembly factors. Although it is known that many of the proteins involved in this process have RNA binding activity, the full repertoire of proteins that interact with the precursor ribosomal RNA is currently unknown.
Piñeiro, David +8 more
openaire +3 more sources
TFIIH is an elongation factor of RNA polymerase I [PDF]
Nucleic Acids Research, 2011 TFIIH is a multisubunit factor essential for transcription initiation and promoter escape of RNA polymerase II and for the opening of damaged DNA double strands in nucleotide excision repair (NER). In this study, we have analyzed at which step of the transcription cycle TFIIH is essential for transcription by RNA polymerase I. We demonstrate that TFIIH
Sebastian Iben +5 more
openaire +3 more sources
The chromatin remodeling factor CSB recruits histone acetyltransferase PCAF to rRNA gene promoters in active state for transcription initiation. [PDF]
PLoS ONE, 2013 The promoters of poised rRNA genes (rDNA) are marked by both euchromatic and heterochromatic histone modifications and are associated with two transcription factors, UBF and SL1 that nucleate transcription complex formation.
Meili Shen +9 more
doaj +1 more source