Research Note: Establishment of vector system harboring duck RNA polymerase I promoter for avian influenza virus. [PDF]
Poult SciReverse genetics (RG) systems are extensively utilized to investigate the characteristics of influenza viruses and develop vaccines, predominantly relying on human RNA polymerase I (pol I). However, the efficiency of RG systems for avian-origin influenza
Jang Y +7 more
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Anti-RNA polymerase I antibodies in sera of MRL lpr/lpr and MRL +/+ autoimmune mice. Correlation of antibody production with delayed onset of lupus-like disease in MRL +/+ mice [PDF]
, 2015 This is the publisher's version, also available electronically from http://jem.rupress.org/content/162/6/1760.Sera from individual MRL/lpr and MRL/++ mice, which develop an autoimmune disease similar to human systemic lupus erythematosus (SLE), were ...
Jacob, Samson T. +2 more
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Structural basis of RNA polymerase I pre-initiation complex formation and promoter melting [PDF]
Nature Communications, 2020 Transcription of the ribosomal RNA precursor by RNA polymerase (Pol) I is a prerequisite for the biosynthesis of ribosomes in eukaryotes. Compared to Pols II and III, the mechanisms underlying promoter recognition, initiation complex formation and DNA ...
Engel, Christoph, Pilsl, Michael
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The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription
eLife, 2017 Cell growth requires synthesis of ribosomal RNA by RNA polymerase I (Pol I). Binding of initiation factor Rrn3 activates Pol I, fostering recruitment to ribosomal DNA promoters.
Eva Torreira +9 more
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TblncRNA-23, a long non-coding RNA transcribed by RNA polymerase I, regulates developmental changes in Trypanosoma brucei. [PDF]
Nat CommunThe protozoan parasite Trypanosoma brucei undergoes a complex life cycle, moving between its mammalian host and the blood-feeding tsetse fly vector. The two major surface proteins expressed by procyclic forms in the insect midgut, EP and GPEET procyclin,
Galili-Kostin B +10 more
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Structural Basis of RNA Polymerase I Transcription Initiation [PDF]
Cell, 2017 Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation.
Engel, Christoph +7 more
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Structure of the initiation-competent RNA polymerase I and its implication for transcription
Nature Communications, 2016 Eukaryotic RNA polymerase I (Pol I) is responsible for the transcription of rRNA genes. Here the authors determine the cryo-EM structure of the Pol I-Rrn3 complex, providing insight into how Rrn3 stabilizes the monomeric initiation competent Pol I to ...
Michael Pilsl +8 more
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Methods Mol Biol, 2022 AbstractIn archaea and bacteria the major classes of RNAs are synthesized by one DNA-dependent RNA polymerase (RNAP). In contrast, most eukaryotes have three highly specialized RNAPs to transcribe the nuclear genome. RNAP I synthesizes almost exclusively ribosomal (r)RNA, RNAP II synthesizes mRNA as well as many noncoding RNAs involved in RNA ...
Merkl PE +8 more
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Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis. [PDF]
PLoS Genetics, 2015 Ribosomal RNA synthesis is controlled by nutrient signaling through the mechanistic target of rapamycin complex 1 (mTORC1) pathway. mTORC1 regulates ribosomal RNA expression by affecting RNA Polymerase I (Pol I)-dependent transcription of the ribosomal ...
R Nicholas Laribee +3 more
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Structural studies of the human RNA polymerase I and the role of human RNA polymerase I and III in diseases. [PDF]
, 2023 In eukaryotes, the task of RNA synthesis is divided between three RNA polymerases (RNAPs). RNA polymerase I (Pol I) produces ribosomal RNA (rRNA), and Pol III specialises in making short, non-transcribed RNAs, which together constitute the majority of ...
Misiaszek, Agata Dorota
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