Results 31 to 40 of about 1,002,934 (332)
A 212-nt long RNA structure in the Tobacco necrosis virus-D RNA genome is resistant to Xrn degradation [PDF]
, 2019 Plus-strand RNA viruses can accumulate viral RNA degradation products during infections. Some of these decay intermediates are generated by the cytosolic 5′-to-3′ exoribonuclease Xrn1 (mammals and yeast) or Xrn4 (plants) and are formed when the enzyme ...
Chaminda, Gunawardene D. +2 more
core +1 more source
Identification of DHX33 as a mediator of rRNA synthesis and cell growth [PDF]
, 2011 In this report, we employed a lentiviral RNA interference screen to discover nucleolar DEAD/DEAH-box helicases involved in RNA polymerase I (Pol I)-mediated transcriptional activity.
Forys, Jason T +4 more
core +3 more sources
Efficient termination of transcription by RNA polymerase I requires the 5 ' exonuclease Rat1 in yeast [PDF]
, 2008 During transcription termination by RNA polymerase II on protein-coding genes, the nuclear 5′ exonuclease Rat1/Xrn2 degrades the nascent transcript downstream from the polyadenylation site and “torpedoes” the polymerase.
El Hage, Aziz +3 more
core +3 more sources
Loss of Proteostasis Is a Pathomechanism in Cockayne Syndrome
Cell Reports, 2018 Summary: Retarded growth and neurodegeneration are hallmarks of the premature aging disease Cockayne syndrome (CS). Cockayne syndrome proteins take part in the key step of ribosomal biogenesis, transcription of RNA polymerase I.
Marius Costel Alupei +11 more
doaj +1 more source
Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription. [PDF]
PLoS ONE, 2017 The nucleolus has long been considered to be a pure ribosome factory. However, over the last two decades it became clear that the nucleolus is involved in numerous other functions besides ribosome biogenesis. Our experiments indicate that the activity of
Robin Assfalg +7 more
doaj +1 more source
Solving the RNA polymerase I structural puzzle [PDF]
Acta Crystallographica Section D Biological Crystallography, 2014 Knowing the structure of multi-subunit complexes is critical to understand basic cellular functions. However, when crystals of these complexes can be obtained they rarely diffract beyond 3 Å resolution, which complicates X-ray structure determination and refinement.
Moreno-Morcillo, Maria +8 more
openaire +5 more sources
Specific Features of RNA Polymerases I and III: Structure and Assembly
Frontiers in Molecular Biosciences, 2021 RNA polymerase I (RNAPI) and RNAPIII are multi-heterogenic protein complexes that specialize in the transcription of highly abundant non-coding RNAs, such as ribosomal RNA (rRNA) and transfer RNA (tRNA).
Tomasz W. Turowski +2 more
doaj +1 more source
RNA polymerase I–Rrn3 complex at 4.8 Å resolution
Nature Communications, 2016 RNA polymerase I is the central enzyme that synthesizes ribosomal RNA in eukaryotic cells, and its regulation underlies cell growth. Here the authors present a high-resolution cryo-EM structure of the Pol I-Rrn3 complex that explains how Rrn3 ...
Christoph Engel +2 more
doaj +1 more source
Viruses, 2019 Flaviviruses, most of which are emerging and re-emerging human pathogens and significant public health concerns worldwide, are positive-sense RNA viruses. Flavivirus replication occurs on the ER and is regulated by many mechanisms and factors. NS5, which
YanPing Duan +17 more
doaj +1 more source
In vivo analysis of NHPX reveals a novel nucleolar localization pathway involving a transient accumulation in splicing speckles [PDF]
, 2002 The NHPX protein is a nucleolar factor that binds directly to a conserved RNA target sequence found in nucleolar box C/D snoRNAs and in U4 snRNA. Using enhanced yellow fluorescent protein (EYFP)– and enhanced cyan fluorescent protein–NHPX fusions, we ...
Lamond, Angus I., Leung, Anthony K. L.
core +5 more sources