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Computational Investigations on Polymerase Actions in Gene Transcription and Replication Combining Physical Modeling and Atomistic Simulations [PDF]
Chin. Phys. B Vol. 25, No. 1 (2016) 018706, 2015 Polymerases are protein enzymes that move along nucleic acid chains and catalyze template-based polymerization reactions during gene transcription and replication. The polymerases also substantially improve transcription or replication fidelity through the non-equilibrium enzymatic cycles.
arxiv +1 more source
Zinc'ing down RNA polymerase I [PDF]
Transcription, 2013 Most RNA polymerases contain zinc, yet the precise function of zinc and its influence of polymerases stability are unknown. A recent study provides evidence that zinc levels control the stability of RNA polymerase I in vivo and that the enzyme might serve as a zinc reservoir for other proteins.
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Autoantibody to RNA polymerase I in scleroderma sera. [PDF]
Journal of Clinical Investigation, 1987 Autoantibodies to components of the nucleolus are a unique serological feature of patients with scleroderma. There are autoantibodies of several specificities; one type produces a speckled pattern of nucleolar staining in immunofluorescence. In actinomycin D and 5,6-dichloro-beta-D-ribofuranosylbenzimidazole-treated Vero cells, staining was restricted ...
Reimer, Georg +3 more
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Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription. [PDF]
PLoS ONE, 2017 The nucleolus has long been considered to be a pure ribosome factory. However, over the last two decades it became clear that the nucleolus is involved in numerous other functions besides ribosome biogenesis. Our experiments indicate that the activity of
Robin Assfalg +7 more
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Stochastic resetting in backtrack recovery by RNA polymerases [PDF]
Phys. Rev. E 93, 062411 (2016), 2016 Transcription is a key process in gene expression, in which RNA polymerases produce a complementary RNA copy from a DNA template. RNA polymerization is frequently interrupted by backtracking, a process in which polymerases perform a random walk along the DNA template.
arxiv +1 more source
Cotranscriptional kinetic folding of RNA secondary structures including pseudoknots [PDF]
Journal of Computational Biology, 2021, 2020 Computational prediction of RNA structures is an important problem in computational structural biology. Studies of RNA structure formation often assume that the process starts from a fully synthesized sequence. Experimental evidence, however, has shown that RNA folds concurrently with its elongation.
arxiv +1 more source
Specific Features of RNA Polymerases I and III: Structure and Assembly
Frontiers in Molecular Biosciences, 2021 RNA polymerase I (RNAPI) and RNAPIII are multi-heterogenic protein complexes that specialize in the transcription of highly abundant non-coding RNAs, such as ribosomal RNA (rRNA) and transfer RNA (tRNA).
Tomasz W. Turowski +2 more
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Termination of transcription by yeast RNA polymerase I [PDF]
Nucleic Acids Research, 1989 Analysis of the termination of transcription by yeast RNA polymerase I (Pol I) using in vitro run-on experiments in both isolated nuclei and permeabilized cells demonstrated that Pol I does not traverse the whole intergenic spacer separating consecutive 37S operons, but terminates transcription before reaching the 5S rRNA gene, that is within NTS 1. In
I. J. De Wijs +6 more
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RNA polymerase I–Rrn3 complex at 4.8 Å resolution
Nature Communications, 2016 RNA polymerase I is the central enzyme that synthesizes ribosomal RNA in eukaryotic cells, and its regulation underlies cell growth. Here the authors present a high-resolution cryo-EM structure of the Pol I-Rrn3 complex that explains how Rrn3 ...
Christoph Engel +2 more
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Viruses, 2019 Flaviviruses, most of which are emerging and re-emerging human pathogens and significant public health concerns worldwide, are positive-sense RNA viruses. Flavivirus replication occurs on the ER and is regulated by many mechanisms and factors. NS5, which
YanPing Duan +17 more
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