Results 41 to 50 of about 807,500 (308)

Mechanical Properties of Transcription [PDF]

Phys. Rev. Lett. 118, 268101 (2017), 2016
Recently the physical characterization of a number of biological processes has proven indispensable for a full understanding of natural phenomena. One such example is the mechanical properties of transcription, which have been shown to have significant effects in gene expression.
arxiv   +1 more source

RNA Polymerase I Holoenzyme-Promoter Interactions [PDF]

Journal of Biological Chemistry, 2000
In plants and animals, RNA polymerase I (pol I) can be purified in a form that is self-sufficient for accurate rRNA gene promoter-dependent transcription and that has biochemical properties suggestive of a single complex, or holoenzyme. In this study, we examined the promoter binding properties of a highly purified Brassica pol I holoenzyme activity ...
Craig S. Pikaard, Julio Sáez-Vásquez
openaire   +3 more sources

Structural mechanism of ATP-independent transcription initiation by RNA polymerase I

eLife, 2017
Transcription initiation by RNA Polymerase I (Pol I) depends on the Core Factor (CF) complex to recognize the upstream promoter and assemble into a Pre-Initiation Complex (PIC). Here, we solve a structure of Saccharomyces cerevisiae Pol I-CF-DNA to 3.8 Å
Yan Han, Chunli Yan, Thi Hoang Duong Nguyen, Ashleigh J Jackobel, Ivaylo Ivanov, Bruce A Knutson, Yuan He   +6 more
doaj   +1 more source

Uncovering the effect of RNA polymerase steric interactions on gene expression noise: analytical distributions of nascent and mature RNA numbers [PDF]

arXiv, 2023
The telegraph model is the standard model of stochastic gene expression, which can be solved exactly to obtain the distribution of mature RNA numbers per cell. A modification of this model also leads to an analytical distribution of the nascent RNA numbers. These solutions are routinely used for the analysis of single-cell data, including the inference
arxiv  

Identification of SL addition trans-splicing acceptor sites in the internal transcribed spacer I region of pre-rRNA in Leishmania (Leishmania) amazonensis

Memorias do Instituto Oswaldo Cruz, 2012
Trypanosomatidae is a family of early branching eukaryotes harbouring a distinctive repertoire of gene expression strategies. Functional mature messenger RNA is generated via the trans-splicing and polyadenylation processing of constitutively transcribed
Mario Gustavo Mayer, Lucile Maria Floeter-Winter   +1 more
doaj   +1 more source

The Phage-Encoded N-Acetyltransferase Rac Mediates Inactivation of Pseudomonas aeruginosa Transcription by Cleavage of the RNA Polymerase Alpha Subunit

Viruses, 2020
In this study, we describe the biological function of the phage-encoded protein RNA polymerase alpha subunit cleavage protein (Rac), a predicted Gcn5-related acetyltransferase encoded by phiKMV-like viruses.
Pieter-Jan Ceyssens, Jeroen De Smet, Jeroen Wagemans, Natalia Akulenko, Evgeny Klimuk, Subray Hedge, Marleen Voet, Hanne Hendrix, Jan Paeshuyse, Bart Landuyt, Hua Xu, John Blanchard, Konstantin Severinov, Rob Lavigne   +13 more
doaj   +1 more source

Mechanisms of backtrack recovery by RNA polymerases I and II [PDF]

Proceedings of the National Academy of Sciences, 2016
Significance Transcription of the genetic information from DNA into RNA is the central process of gene expression, and it is performed by enzymes called RNA polymerases (Pol). Transcription is interspersed with a proofreading mechanism called backtracking, during which the polymerase moves backward on the DNA template and displaces the RNA 3′
Eric A. Galburt, Édgar Roldán, Patrick Cramer, Christoph Engel, Marcus Jahnel, Stephan W. Grill, Stephan W. Grill, Ana Lisica   +7 more
openaire   +6 more sources

Structural Basis of RNA Polymerase I Transcription Initiation [PDF]

Cell, 2017
Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation.
Engel, Christoph, Gubbey, Tobias, Neyer, Simon, Sainsbury, Sarah, Oberthuer, Christiane, Baejen, Carlo, Bernecky, Carrie, Cramer, Patrick   +7 more
openaire   +5 more sources

miRNA‐29 regulates epidermal and mesenchymal functions in skin repair

FEBS Letters, EarlyView.
miRNA‐29 inhibits cell‐to‐cell and cell‐to‐matrix adhesion by silencing mRNA targets. Adhesion is controlled by complex interactions between many types of molecules coded by mRNAs. This is crucial for keeping together the layers of the skin and for regenerating the skin after wounding.
Lalitha Thiagarajan, Rosa Sanchez‐Alvarez, Chiho Kambara, Poojitha Rajasekar, Yuluang Wang, François Halloy, Jonathan Hall, Hans‐Jürgen Stark, Iris Martin, Petra Boukamp, Svitlana Kurinna   +10 more
wiley   +1 more source

Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast.

PLoS Genetics, 2021
The adjustment of transcription and translation rates to the changing needs of cells is of utmost importance for their fitness and survival. We have previously shown that the global transcription rate for RNA polymerase II in budding yeast Saccharomyces ...
José E Pérez-Ortín, Adriana Mena, Marina Barba-Aliaga, Abhyudai Singh, Sebastián Chávez, José García-Martínez   +5 more
doaj   +1 more source