Results 81 to 90 of about 950,582 (330)
Interchangeable RNA polymerase I and II enhancers. [PDF]
Proceedings of the National Academy of Sciences, 1990 The RNA polymerase I (pol I) enhancer of Saccharomyces cerevisiae contains at least three elements commonly associated with RNA polymerase II (pol II) enhancers, binding sites for the transcriptional activators general regulatory factor 2 and autonomously replicating sequence-binding factor I, and a thymidine-rich element.
Neal F. Lue +2 more
openaire +3 more sources
Molecular Oncology, EarlyView.We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau +11 more
wiley +1 more source
Isolation of TFIIIC102 in Arabidopsis thaliana to better understand transcription [PDF]
, 2004 only availableIn eukaryotes, transcription is carried out by three RNA polymerases. RNA polymerase I (Pol I) transcribes genes encoding the large rRNAs, RNA Pol II transcribes genes encoding proteins involved in mRNA processing, and RNA Pol III ...
Harrington, Lynne
core
Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources
Molecular Oncology, EarlyView.This study highlights the importance of multi‐omic analyses in characterizing colorectal cancers. Indeed, our analysis revealed a rare CMS1 exhibiting dampened immune activation, including reduced PD‐1 expression, moderate CD8+ T‐cell infiltration, and suppressed JAK/STAT pathway.
Livia Concetti +10 more
wiley +1 more source
Is the Intergenic Region of Aedes aegypti Totivirus a Recombination Hotspot?
Viruses, 2022 The genus totivirus in the family Totiviridae contains double-stranded RNA viruses. Their genome has two open reading frames (ORFs) that encode capsid protein (CP) and RNA-dependent RNA polymerase (RdRp).
Roseane da Silva Couto +3 more
doaj +1 more source
Nascent RNA sequencing reveals mechanisms of gene regulation in the human malaria parasite Plasmodium falciparum. [PDF]
, 2017 Gene expression in Plasmodium falciparum is tightly regulated to ensure successful propagation of the parasite throughout its complex life cycle. The earliest transcriptomics studies in P.
Batugedara, Gayani +5 more
core +1 more source
RNA Polymerase from the Fungus Aspergillus nidulans [PDF]
European Journal of Biochemistry, 1979 The DNA‐dependent RNA polymerase I (or A) from the lower eukaryote Aspergillus nidulans has been purified on a large scale to apparent homogeneity by homogenizing the fungal hyphae in liquid nitrogen, extraction of the enzyme at high salt concentration, precipitation of RNA polymerase activity with polymin P (a polyethylene imine), elution of the RNA ...
Stunnenberg, H.G. +2 more
openaire +3 more sources
Molecular Oncology, EarlyView.Dual targeting of AKT and mTOR using MK2206 and RAD001 reduces tumor burden in an intracardiac colon cancer circulating tumor cell xenotransplantation model. Analysis of AKT isoform‐specific knockdowns in CTC‐MCC‐41 reveals differentially regulated proteins and phospho‐proteins by liquid chromatography coupled mass spectrometry. Circulating tumor cells
Daniel J. Smit +19 more
wiley +1 more source
The Replicase Protein of Potato Virus X Is Able to Recognize and Trans-Replicate Its RNA Component
VirusesThe trans-replication system explores the concept of separating the viral RNA involved in the translation of the replicase protein from the replication of the viral genome and has been successfully used to study the replication mechanisms of alphaviruses.
Pinky Dutta +3 more
doaj +1 more source