Results 91 to 100 of about 991,885 (339)
Structural basis of the nucleosome transition during RNA polymerase II passage
Science, 2018 Nucleosomal DNA transcription In eukaryotes, the basic chromatin unit nucleosome stalls RNA polymerase II (RNAPII) when it transcribes genetic information on DNA. Using cryo–electron microscopy, Kujirai et al.
T. Kujirai +5 more
semanticscholar +1 more source
Extragenic Accumulation of RNA Polymerase II Enhances Transcription by RNA Polymerase III
PLoS Genetics, 2007 Recent genomic data indicate that RNA polymerase II (Pol II) function extends beyond conventional transcription of primarily protein-coding genes. Among the five snRNAs required for pre-mRNA splicing, only the U6 snRNA is synthesized by RNA polymerase III (Pol III).
Neugebauer, Karla M. +3 more
openaire +6 more sources
Solid phase chemistry to covalently and reversibly capture thiolated RNA. [PDF]
, 2018 Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s4U) metabolic labeling and solid phase chemistry.
Canzio, Daniele +3 more
core +1 more source
Molecular Oncology, EarlyView.CDK11 inhibition stabilises the tumour suppressor p53 and triggers the production of an alternative p21WAF1 splice variant p21L, through the inactivation of the spliceosomal protein SF3B1. Unlike the canonical p21WAF1 protein, p21L is localised in the cytoplasm and has reduced cell cycle‐blocking activity.
Radovan Krejcir +12 more
wiley +1 more source
CDK9-dependent RNA polymerase II pausing controls transcription initiation
eLife, 2017 Gene transcription can be activated by decreasing the duration of RNA polymerase II pausing in the promoter-proximal region, but how this is achieved remains unclear.
Saskia Gressel +6 more
semanticscholar +1 more source
Divergent RNA transcription:A role in promoter unwinding? [PDF]
, 2013 New approaches using biotinylated-psoralen as a probe for investigating DNA structure have revealed new insights into the relationship between DNA supercoiling, transcription and chromatin compaction.
Corless, Samuel +2 more
core +1 more source
FEBS Open Bio, EarlyView.TTT and R2TP chaperone complexes are required for the assembly and activation of mTORC1. WAC directly interacts with components of TTT, R2TP, and mTORC1, and these interactions are affected by the availability of glucose and glutamine, correlating with changes in mTORC1 activity.
Sofía Cabezudo +11 more
wiley +1 more source
Seapration of B. subtilis DNA into complementary strands. II. Template functions and composition as determined by transcription with RNA polymerase. [PDF]
, 1968 J D Karkas, Rivka Rudner, Erwin Chargaff
openalex +1 more source
Xrn2 accelerates termination by RNA polymerase II, which is underpinned by CPSF73 activity
Genes & Development, 2018 In this study, Eaton et al. use a new gene-editing approach to delineate the mechanism by which RNA polymerase II terminates. They generated human cell lines from which the 5′-to-3′ exoribonuclease Xrn2 or the poly(A) signal endoribonuclease CPSF73 can ...
Joshua D. Eaton +5 more
semanticscholar +1 more source
Structure of transcribing mammalian RNA polymerase II
Nature, 2016 RNA polymerase (Pol) II produces messenger RNA during transcription of protein-coding genes in all eukaryotic cells. The Pol II structure is known at high resolution from X-ray crystallography for two yeast species. Structural studies of mammalian Pol II, however, remain limited to low-resolution electron microscopy analysis of human Pol II and its ...
Carrie Bernecky +4 more
openaire +5 more sources