Results 91 to 100 of about 946,604 (388)

Solid phase chemistry to covalently and reversibly capture thiolated RNA. [PDF]

, 2018
Here, we describe an approach to enrich newly transcribed RNAs from primary mouse neurons using 4-thiouridine (s4U) metabolic labeling and solid phase chemistry.
Canzio, Daniele, Duffy, Erin E, Maniatis, Tom, Simon, Matthew D   +3 more
core   +1 more source

The punctilious RNA polymerase II core promoter [PDF]

Genes & Development, 2017
The signals that direct the initiation of transcription ultimately converge at the core promoter, which is the gateway to transcription. Here we provide an overview of the RNA polymerase II core promoter in bilateria (bilaterally symmetric animals). The core promoter is diverse in terms of its composition and function yet is also punctilious, as it ...
ngoc, Long Vo, Wang, Yuan-Liang, Kassavetis, George A, Kadonaga, James T   +3 more
openaire   +5 more sources

KRAS and GNAS mutations in cell‐free DNA and in circulating epithelial cells in patients with intraductal papillary mucinous neoplasms—an observational pilot study

Molecular Oncology, EarlyView.
This study demonstrates that KRAS and GNAS mutations are more prevalent in patients with resected intraductal papillary mucinous neoplasms (IPMN) compared to those under clinical surveillance. GNAS mutations significantly differ between the two patient cohorts, indicating that their absence may serve as a potential biomarker to support conservative ...
Christine Nitschke, Marie Tölle, Philipp Walter, Kira Meißner, Mara Goetz, Jolanthe Kropidlowski, Andreas W. Berger, Jakob R. Izbicki, Felix Nickel, Thilo Hackert, Klaus Pantel, Harriet Wikman, Faik G. Uzunoglu   +12 more
wiley   +1 more source

Ups and Downs of Poised RNA Polymerase II in B-Cells. [PDF]

PLoS Computational Biology, 2016
Recent genome-wide analyses have uncovered a high accumulation of RNA polymerase II (Pol II) at the 5' end of genes. This elevated Pol II presence at promoters, referred to here as Poll II poising, is mainly (but not exclusively) attributed to temporal ...
Phuong Dao, Damian Wojtowicz, Steevenson Nelson, David Levens, Teresa M Przytycka   +4 more
doaj   +1 more source

Transcription start site scanning and the requirement for ATP during transcription initiation by RNA polymerase II [PDF]

, 2016
Saccharomyces cerevisiae RNA polymerase (Pol) II locates transcription start sites (TSS) at TATA-containing promoters by scanning sequences downstream from the site of preinitiation complex formation, a process that involves the translocation of ...
Fishburn, James, Galburt, Eric, Hahn, Steven   +2 more
core   +2 more sources

N4 RNA Polymerase II, a Heterodimeric RNA Polymerase with Homology to the Single-Subunit Family of RNA Polymerases [PDF]

Journal of Bacteriology, 2002
ABSTRACTBacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4 early promoter Pe3.
R. H. Carter, Krystyna M Kazmierczak, S. H. Willis, Lucia B. Rothman-Denes   +3 more
openaire   +3 more sources

Structure and mechanism of the RNA polymerase II transcription machinery

Genes & Development, 2020
In this review, Schier et al. summarize the structural and functional data that have enabled a deeper understanding of Pol II transcription mechanisms; they also highlight mechanistic questions that remain unanswered or controversial.
Allison C. Schier, D. Taatjes
semanticscholar   +1 more source

Cellular liquid biopsy provides unique chances for disease monitoring, preclinical model generation and therapy adjustment in rare salivary gland cancer patients

Molecular Oncology, EarlyView.
We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić, Florian Lüke, Steffi Treitschke, Andrea Coluccio, Martin Hoffmann, Giancarlo Feliciello, Adithi Ravikumar Varadarajan, Xin Lu, Kathrin Weidele, Catherine Botteron, Silvia Materna–Reichelt, Felix Keil, Katja Evert, Florian Weber, Thomas Schamberger, Michael Althammer, Jirka Grosse, Dirk Hellwig, Christian Schulz, Stephan Seitz, Peter Ugocsai, Anke Schlenska‐Lange, Roman Mayr, Ulrich Kaiser, Wolfgang Dietmaier, Bernhard Polzer, Jens Warfsmann, Kamran Honarnejad, Tobias Pukrop, Daniel Heudobler, Christoph A. Klein, Christian Werno   +31 more
wiley   +1 more source

A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. [PDF]

, 2019
Elevated glucose consumption is fundamental to cancer, but selectively targeting this pathway is challenging. We develop a high-throughput assay for measuring glucose consumption and use it to screen non-small-cell lung cancer cell lines against ...
Chen, Bao Ying, Clark, Peter M, Damoiseaux, Robert, Ghezzi, Chiara, Ribalet, Bernard, Wong, Alicia   +5 more
core   +1 more source

Combinatorial Recruitment of CREB, C/EBPβ and c-Jun Determines Activation of Promoters upon Keratinocyte Differentiation [PDF]

, 2013
Background: Transcription factors CREB, C/EBPβ and Jun regulate genes involved in keratinocyte proliferation and differentiation. We questioned if specific combinations of CREB, C/EBPβ and c-Jun bound to promoters correlate with RNA polymerase II binding,
Bhattacharya, Paramita, Chatterjee, Raghunath, Glass, Kimberly, Rozenberg, Julian M., Vinson, Charles   +4 more
core   +3 more sources