Results 261 to 270 of about 602,650 (303)

Probing RNA Structure Within Living Cells

2009
RNA folding is the most fundamental process underlying RNA function. RNA structure and associated folding paradigms have been intensively studied in vitro. However, in vivo RNA structure formation has only been explored to a limited extent. To determine the influence of the cellular environment, which differs significantly from the in vitro refolding ...
Liebeg, Andreas, Waldsich, Christina
openaire   +3 more sources

Chemical Probing of RNA and RNA/Protein Complexes

2009
Chemical probing is widely used as a rapid approach for assessing RNA structure, folding, and function. In this chapter, we outline procedures for handling and using chemicals commonly used to probe nucleic acids. Detailed experimental conditions and design for footprinting and modification interference are presented herein.
Zhili, Xu, Gloria M, Culver
openaire   +2 more sources

Antisense probing of dynamic RNA structures

Methods, 2020
RNA regulation is influenced by the dynamic changes in conformational accessibility on the transcript. Here we discuss the initial validation of a cell-free antisense probing method for structured RNAs, using the Tetrahymena group I intron as a control target.
Alexandra J, Lukasiewicz, Lydia M, Contreras   +1 more
openaire   +2 more sources

Modern methods for probing RNA structure

Current Opinion in Biotechnology, 1998
Molecular biologists have been remarkably successful in dividing large RNAs into small functional modules manageable for NMR and X-ray studies. At the same time biophysical, biochemical and genetic tools in RNA structure determination have reached a level of sophistication, at which we start to see a glimpse of molecular dynamics and the mechanism of ...
J, Kjems, J, Egebjerg
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RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing

2019
RNA structure probing enables the characterization of RNA secondary structures by established procedures such as the enzyme- or chemical-based detection of single- or double-stranded regions. A specific type of application involves the detection of changes of RNA structures and conformations that are induced by proteins with RNA chaperone activity ...
Susann, Friedrich, Tobias, Schmidt, Sven-Erik, Behrens   +2 more
openaire   +2 more sources

Genetic probes of ribosomal RNA function

Biochemistry and Cell Biology, 1995
We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation.
M, O'Connor, C A, Brunelli, M A, Firpo, S T, Gregory, K R, Lieberman, J S, Lodmell, H, Moine, D I, Van Ryk, A E, Dahlberg   +8 more
openaire   +2 more sources

In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe

2000
Non-radioactive in situ hybridization method is now widely used to detect nucleic acid in tissue sections. However, the results for the detection of tissue mRNA are not always consistent. We have to consider several factors for this purpose. We describe our current methods for the most consistent detection.
openaire   +1 more source

Probing Small Non-Coding RNAs Structures

2015
The diverse roles of RNAs depend on their ability to fold so as to form biologically functional structures. Thus, understanding the function of a given RNA molecule often requires experimental analysis of its secondary structure by in vitro RNA probing, which is more accurate than using prediction programs only.
Jean-Vincent, Philippe, Lilia, Ayadi, Christiane, Branlant, Isabelle, Behm-Ansmant   +3 more
openaire   +2 more sources

Solution Structure Probing of RNA Structures

2008
Single-stranded RNA plant viruses not only code for viral proteins within their RNA genomes, they often maintain elaborate RNA secondary structures. These structures can be integral to a variety of viral processes, such as viral translation, genome replication, subgenomic mRNA transcription, and genome encapsidation.
Marc R, Fabian, K Andrew, White
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Use of RNA Probes to Detect Plant RNA Viruses

1986
Publisher Summary This chapter discusses the use of RNA probes to detect plant RNA viruses. The detection and diagnosis of plant viral disease agents by nucleic acid hybridization is a valuable technique. This simple and sensitive hybridization assay permits the rapid analysis of a large number of individual plant samples for viral and viral-like ...
S.J. Garger, T.H. Turpen
openaire   +1 more source