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RNA Sequencing Analysis of Saliva exRNA

2022
Next-generation sequencing (NGS) methodologies are rapidly developing. However, RNA Sequencing of saliva is challenging due to low abundance and integrity of extracellular RNA, as well as large amounts of bacterial RNAs that may be encountered in saliva. In addition, the literature about human salivary extracellular RNA is very scarce.
Karolina Elżbieta, Kaczor-Urbanowicz   +1 more
openaire   +2 more sources

Alternative expression analysis by RNA sequencing

Nature Methods, 2010
In alternative expression analysis by sequencing (ALEXA-seq), we developed a method to analyze massively parallel RNA sequence data to catalog transcripts and assess differential and alternative expression of known and predicted mRNA isoforms in cells and tissues.
Malachi, Griffith   +25 more
openaire   +2 more sources

RNA SEQUENCE ANALYSIS

Annual Review of Biochemistry, 1970
FRACTIONATION AND PURIFICATION OF RNA 228 Transfer RNA 228 Ribosomal RNA ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 230 Viral RNA 230 SEPARATION AND ISOLATION OF POLYNUCLEOTIDES 231 Physical methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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RNA sequencing and Prediction Tools for Circular RNAs Analysis

2018
Circular RNAs (circRNAs) are noncoding and single-stranded RNA transcripts able to form covalently circular-closed structures. They are generated through alternative splicing events and widely expressed from human to viruses. CircRNAs have been appointed as potential regulators of microRNAs (miRNAs), RNA-binding proteins (RPBs), and lineal protein ...
Elena, López-Jiménez   +2 more
openaire   +2 more sources

Massively Parallel Analysis of Regulatory RNA Sequences

2021
The stability of RNA transcripts is regulated by signals within their sequences, but the identity of those signals still remain elusive in many biological systems. Recently introduced massively parallel tools for the analysis of regulatory RNA sequences provide the ability to detect functional cis-regulatory sequences of post-transcriptional RNA ...
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Vaccinia Virus Transcriptome Analysis by RNA Sequencing

2019
RNA-sequencing (RNA-Seq) using next-generation sequencing (NGS) technique is a powerful tool for simultaneous analysis of global transcripts from both vaccinia virus and host cell. Here, we describe an RNA-Seq method for analyzing the vaccinia virus transcriptome from virus-infected HeLa cells.
Shuai, Cao, Yongquan, Lin, Zhilong, Yang
openaire   +2 more sources

Borrelia burgdorferi Transcriptome Analysis by RNA-Sequencing

2017
Next-Generation Sequencing (NGS) has revolutionized transcriptomics studies in the last decade. Transcriptome analysis experiments using NGS-based RNA-sequencing have several advantages over DNA microarray analysis. Novel unannotated transcripts and transcriptional start sites can be identified.
M, Lybecker, K C, Henderson
openaire   +2 more sources

Markov analysis of viral DNA/RNA sequences

Journal of Theoretical Biology, 1980
Abstract This work applies a previously published method for determining the order of a Markov chain to the DNA/RNA sequences of φX174, SV40 and MS2. In the first two cases rather long-range order is found—third and second order respectively—but zero order is the appropriate model for MS2.
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RNA Sequencing: From Sample Preparation to Analysis

2014
The introduction of RNA sequencing (RNA-seq), as a direct result of rapid progression of next-generation sequencing technologies, has revolutionized the world of transcriptomics. It enables quantification of the complete set of RNA with all its isoforms in a given cell, in far greater accuracy than before.
Sho, Maekawa   +3 more
openaire   +2 more sources

Bioinformatic Analysis of Small RNA Sequencing Libraries

2019
Bioinformatic analysis of small RNA sequencing libraries consists of transforming a series of small RNA sequencing experiment fastq files into a table containing small RNA sequences and their abundance. This is achieved by cleaning the reads, aligning the cleaned reads to a reference, and parsing the alignment results.
Ricardo A, Chávez Montes   +2 more
openaire   +2 more sources

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