Results 311 to 320 of about 1,674,122 (352)

RNA Sequencing Analysis of Saliva exRNA

2022
Next-generation sequencing (NGS) methodologies are rapidly developing. However, RNA Sequencing of saliva is challenging due to low abundance and integrity of extracellular RNA, as well as large amounts of bacterial RNAs that may be encountered in saliva. In addition, the literature about human salivary extracellular RNA is very scarce.
Karolina Elżbieta, Kaczor-Urbanowicz, David T W, Wong   +1 more
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[30] Methods of RNA sequence analysis

1983
Publisher Summary Rapid methods for the determination of the nucleotide sequence of DNA by base-specific chemical cleavage I and chain termination by dideoxynucleotides are in wide use. Similar techniques have been developed for RNA sequence analysis using base specific cleavage by both chemical and enzymic methods as well as chain termination with ...
Alan M. Diamond, Bernard Dudock
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RNA SEQUENCE ANALYSIS

Annual Review of Biochemistry, 1970
FRACTIONATION AND PURIFICATION OF RNA 228 Transfer RNA 228 Ribosomal RNA ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 230 Viral RNA 230 SEPARATION AND ISOLATION OF POLYNUCLEOTIDES 231 Physical methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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Sequence and Structure Analysis of Noncoding RNAs

2009
Noncoding RNAs (ncRNAs) are increasingly recognized as important functional molecules in the cell. Here we give a short overview of fundamental computational techniques to analyze ncRNAs that can help us better understand their function. Topics covered include prediction of secondary structure from the primary sequence, prediction of consensus ...
Stefan Washietl, Stefan Washietl
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RNA sequencing and Prediction Tools for Circular RNAs Analysis

2018
Circular RNAs (circRNAs) are noncoding and single-stranded RNA transcripts able to form covalently circular-closed structures. They are generated through alternative splicing events and widely expressed from human to viruses. CircRNAs have been appointed as potential regulators of microRNAs (miRNAs), RNA-binding proteins (RPBs), and lineal protein ...
Eduardo Andrés-León, Elena López-Jiménez, Ana M. Rojas   +2 more
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Design and Analysis of RNA Sequencing Data

2021
In this chapter, we introduce the concept of RNA-Seq analyses. First, we start to provide an overview of a typical RNA-Seq experiment that includes extraction of sample RNA, enrichment, and cDNA library preparation. Next, we review tools for quality control and data pre-processing followed by a standard workflow to perform RNA-Seq analyses.
Dominik G. Grimm, Dominik G. Grimm, Richa Bharti, Richa Bharti   +3 more
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Analysis of RNA Transcripts by High-Throughput RNA Sequencing

2012
For many years, array-based methods have been used successfully to interrogate the transcriptome of the cell. These methods rely on quantification of the hybridization intensity of the interrogated RNAs to predesigned probes. Recently emerging methods have adopted massively parallel sequencing technologies - also known as Next Generation Sequencing ...
Ribeca, Paolo, Lacroix, Vincent, Sammeth, Michael, Guigó, Roderic   +3 more
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Identification and Analysis of Palindromes for RNA Sequences

2016 IEEE 16th International Conference on Bioinformatics and Bioengineering (BIBE), 2016
A palindrome is a string of the form S = A1 A2 or S = A1 aA2, where A1 and A2 are substrings of S and the reverse of A2 exactly matches with A1. DNA palindromes show direct influence on tumerogenesis due to the formation of DNA palindromes at microRNA genes, which involve in tumor development. Furthermore, RNA palindromes play a crucial role in genomic
Chien-Hung Huang, Nilubon Kurubanjerdjit, Ka-Lok Ng, Jeffrey J. P. Tsai, Jywe-Fei Fang, Praveen Kumar Korla, Sheng-Jia Weng   +6 more
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In Silico Analysis of Micro-RNA Sequencing Data

2021
High-throughput sequencing for micro-RNAs (miRNAs) to obtain expression estimates is a central method of molecular biology. Surprisingly, there are a number of different approaches to converting sequencing output into micro-RNA counts. Each has their own strengths and biases that impact on the final data that can be obtained from a sequencing run. This
Michael Hackenberg, Marc K. Halushka, Ernesto Aparicio-Puerta, Bastian Fromm   +3 more
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RNA Sequencing: From Sample Preparation to Analysis [PDF]

, 2014
The introduction of RNA sequencing (RNA-seq), as a direct result of rapid progression of next-generation sequencing technologies, has revolutionized the world of transcriptomics. It enables quantification of the complete set of RNA with all its isoforms in a given cell, in far greater accuracy than before.
Sumio Sugano, Yutaka Suzuki, Sho Maekawa, Ayako Suzuki   +3 more
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