Results 91 to 100 of about 1,474,432 (298)

RnaseIII and T4 Polynucleotide Kinase Sequence Biases and Solutions During RNA-Seq Library Construction [PDF]

, 2013
Background: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement.
Changhoon Lee, Claus O Wilke, Jason K Wall, Lee, Changhoon, R Adron Harris, R Dayne Mayfield   +5 more
core   +3 more sources

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source

rMAPS: RNA map analysis and plotting server for alternative exon regulation. [PDF]

, 2016
RNA-binding proteins (RBPs) play a critical role in the regulation of alternative splicing (AS), a prevalent mechanism for generating transcriptomic and proteomic diversity in eukaryotic cells.
Jung, Sungbo, Park, Juw Won, Rouchka, Eric C, Tseng, Yu-Ting, Xing, Yi   +4 more
core   +2 more sources

Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin

Molecular Oncology, EarlyView.
The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla, Jan Kosla, Magdalena Prechova, Lukas Frick, Patricia Bortel, Yasmin Borutzki, Andrea Bileck, Christopher Gerner, Samuel M. Meier‐Menches, Selma Osmanagic‐Myers, Martin Gregor   +10 more
wiley   +1 more source

Analysis of Differentially Expressed Long Noncoding RNA in Renal Ischemia-Reperfusion Injury

Kidney & Blood Pressure Research, 2020
Background: Renal ischemia-reperfusion (IR) injury is one of the major causes of acute renal failure which seriously endangers the health and life of patients.
Fen Liu, Yang Yang, Tong Liu, Jun Deng, Heng Zhang, Dan Luo, Yuan-Lei Lou   +6 more
doaj   +1 more source

Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics [PDF]

, 2014
We propose a neural connectomics strategy called Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC), leveraging fluorescent in situ nucleic acid sequencing in fixed tissue (FISSEQ).
Boyden, Edward S, Church, George M, Daugharthy, Evan R, Kalhor, Reza, Kebschull, Justus M, Kording, Konrad P, Lee, Je Hyuk, Marblestone, Adam H, Mishchenko, Yuriy, Peikon, Ian D, Shipman, Seth L, Zador, Anthony M   +11 more
core  

An optimized methodology for whole genome sequencing of RNA respiratory viruses from nasopharyngeal aspirates [PDF]

, 2018
Over the last decade, the number of viral genome sequences deposited in available databases has grown exponentially. However, sequencing methodology vary widely and many published works have relied on viral enrichment by viral culture or nucleic acid ...
Goya, Stephanie, Greninger, Alexander L., Marti, Marcelo Adrian, Mistchenko, Alicia Susana, Nabaes Jodar, Mercedes Soledad, Rojo, Gabriel Lihue, Tittarelli, Estefanía, Valinotto, Laura Elena, Viegas, Mariana, Zaiat, Jonathan Javier   +9 more
core   +2 more sources

Dammarenediol II enhances etoposide‐induced apoptosis by targeting O‐GlcNAc transferase and Akt/GSK3β/mTOR signaling in liver cancer

Molecular Oncology, EarlyView.
Etoposide induces DNA damage, activating p53‐dependent apoptosis via caspase‐3/7, which cleaves PARP1. Dammarenediol II enhances this apoptotic pathway by suppressing O‐GlcNAc transferase activity, further decreasing O‐GlcNAcylation. The reduction in O‐GlcNAc levels boosts p53‐driven apoptosis and influences the Akt/GSK3β/mTOR signaling pathway ...
Jaehoon Lee, Byung‐Cheol Han, Gi‐Bang Koo, Jihye Park, Mijin Kwon, Young Bin Park, Jae‐Mun Choi, Seung‐Ho Lee, Sangho Roh   +8 more
wiley   +1 more source

Rapid Genome Sequencing of RNA Viruses

Emerging Infectious Diseases, 2007
We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors.
Tetsuya Mizutani, Daiji Endoh, Michiko Okamoto, Kazuya Shirato, Hiroyuki Shimizu, Minetaro Arita, Shuetsu Fukushi, Masayuki Saijo, Chang Kweng Lim, Mikako Ito, Reiko Nerome, Tomohiko Takasaki, Koji Ishii, Tetsuro Suzuki, Ichiro Kurane, Shigeru Morikawa, Hidekazu Nishimura   +16 more
doaj   +1 more source

Models for transcript quantification from RNA-Seq [PDF]

, 2011
RNA-Seq is rapidly becoming the standard technology for transcriptome analysis. Fundamental to many of the applications of RNA-Seq is the quantification problem, which is the accurate measurement of relative transcript abundances from the sequenced reads.
Pachter, Lior
core