Results 131 to 140 of about 219,883 (262)

A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells

Advanced Science, EarlyView.
A Stringent dUPlex‐activated Error Robust (SUPER) DNAzyme system enables real‐time imaging of alternative mRNA splicing (e.g., Bcl‐xL/Bcl‐xS) in living cells via target‐triggered split‐DNAzyme reassembly and dual‐color fluorescence. It also achieves mRNA‐selective knockdown through DNAzyme‐based gene regulation, serving as a versatile tool for splicing
Mengru Lin, Jiale Sun, Yuqing Mao, Yuhao Tang, Fuan Wang, Zhihong Liu, Jing Wang   +6 more
wiley   +1 more source

Visualizing RNA splicing [PDF]

Nature Reviews Molecular Cell Biology, 2013
openaire   +1 more source

A study of RNA splicing and protein expression in the living human brain. [PDF]

PLoS One
Kopell BH, Kaji DA, Liharska LE, Vornholt E, Lund A, Hashemi A, Thompson RC, Johnson JS, Bussola N, Cheng E, Park YJ, Shah P, Ma W, Searfoss R, Miller GM, Chand NM, Humphrey J, Wilkins L, Ziafat K, Silk H, Linares LM, Sullivan B, Feng C, Cohen V, Kota P, Moya E, Rieder MK, Nadkarni GN, Breen MS, Scarpa J, Narain NR, Wang P, Kiebish MA, Schadt EE, Beckmann ND, Charney AW.   +35 more
europepmc   +1 more source

Genetic Diagnosis and Discovery Enabled by Large Language Models

Advanced Science, EarlyView.
We demonstrate that large language models (LLMs) can facilitate genetic diagnosis and discovery. LLMs were used to solve four types of genetic problems of sequentially increased complexity. An LLM‐based pipeline could analyze genetic variants in the genomic sequences of human hearing loss or rare genetic disease patients and assist in identifying ...
Tao Tu, Khaled Saab, Weida Liu, Zhouqing Fang, Zhuanfen Cheng, Svetolik Spasic, Maja Djurisic, Hiroaki Mohri, Wenlong Ren, Anil Palepu, Juraj Gottweis, Alan Karthikesalingam, Kavita Kulkarni, Annalisa Pawlosky, Devon Bonner, Elijah Kravets, Shruti Marwaha, Hector R. Mendez, Matthew T. Wheeler, Jonathan A. Bernstein, Undiagnosed Diseases Network, Cheng‐Yu Tsai, Chen‐Chi Wu, Konstantina M. Stankovic, Vivek Natarajan, Gary Peltz   +25 more
wiley   +1 more source

A deep dive into statistical modeling of RNA splicing QTLs reveals variants that explain neurodegenerative disease. [PDF]

Am J Hum Genet
Wang D, Gazzara MR, Jewell S, Wales-McGrath BD, Yang K, Brown CD, Choi PS, Barash Y.   +7 more
europepmc   +1 more source

TGFβ1 Activates Lnc‐APUE to Promote Tumor Metastasis via the Alu Element‐Driven STAU1‐Mediated Decay of CDH1 mRNA

Advanced Science, EarlyView.
We delineate a TGFβ1/SMAD/lnc‐APUE/E‐cadherin axis wherein TGFβ1 signaling activates lnc‐APUE; lnc‐APUE subsequently downregulates E‐cadherin expression via Alu element‐driven STAU1‐mediated decay of CDH1 mRNA, thereby promoting tumor metastasis. Our findings reveal a novel regulatory function for lnc‐APUE within the SMD pathway, elucidate a distinct ...
Song‐Yang Li, Jia‐Hui Huang, Jin‐E Yang, Yi‐Hang Li, Juan‐Zhen Hong, Ting‐Ting Wang, Ying‐Lei Chi, Meng‐Zhi Wu, Wei Wang, Ying Zhu, Shi‐Mei Zhuang   +10 more
wiley   +1 more source

FIR/PUF60: Multifunctional Molecule Through RNA Splicing for Revealing the Novel Disease Mechanism and Effective Individualized Therapies. [PDF]

Int J Mol Sci
Matsushita K, Kitamura K, Tanaka N, Kobayashi S, Suenaga Y, Hoshino T.   +5 more
europepmc   +1 more source

Ascites circRNA ASCOR Drives Platinum Resistance of High‐Grade Serous Ovarian Cancer by Facilitating RPA1 Nuclear Translocation

Advanced Science, EarlyView.
High‐grade serous ovarian carcinoma (HGSOC) is plagued by platinum resistance, with malignant ascites closely linked to poor outcomes. Here, we identify ASCOR, a circRNA enriched in ascites small extracellular vesicles (sEVs) from platinum‐resistant HGSOC patients, as a predictor of poor survival. ASCOR enhances platinum resistance in vitro and in vivo
Hanyuan Liu, Chenchen Zhu, Xuelin Yao, Yi Liu, Jing Zhu, Yuebo Li, Hanjie Xu, Cheng Peng, Ge Shan, Liang Chen, Ying Zhou   +10 more
wiley   +1 more source

Activation and Spatial Redistribution of RNA Splicing Factors Trigger Hepatic Regeneration. [PDF]

Cell Mol Gastroenterol Hepatol
An Y, Lian J, Wei W, Mao Y, Qiao L, Li T, Li R, Li S, Zhao S, Chen X, Zhao H, Hu H.   +11 more
europepmc   +1 more source

Elevated TRIM25 Impairs Poly (ADP‐ribose) Metabolism via PARG Degradation and Mediates Compression‐Induced Intervertebral Disc Degeneration

Advanced Science, EarlyView.
TRIM25 acts as a multifunctional hub driving intervertebral disc degeneration under mechanical stress. Mechanical compression significantly upregulates TRIM25 expression, establishing it as a key E3 ubiquitin ligase platform. TRIM25 targets PARG and Ku80 via distinct molecular interfaces, triggering their ubiquitination and degradation.
Zhangrong Cheng, Haiyang Gao, Wenbo Wu, Pengzhi Shi, Xianglong Chen, Zimu Yu, Wang Wu, Kangcheng Zhao, Cao Yang, Yukun Zhang   +9 more
wiley   +1 more source