Results 41 to 50 of about 219,883 (262)

RNA splicing: Unexpected spliceosome diversity [PDF]

Current Biology, 1996
A novel form of spliceosome, containing the minor snRNPs U11 and U12, splices a class of pre-mRNA introns with non-consensus splice sites. This unexpected spliceosome diversity has interesting implications for the evolution and expression of eukaryotic genes.
Kreivi, Jan Peter, Lamond, Angus I.
openaire   +3 more sources

Expression of Herpes Simplex Virus Thymidine Kinase/Ganciclovir by RNA Trans-Splicing Induces Selective Killing of HIV-Producing Cells

Molecular Therapy: Nucleic Acids, 2017
Antiviral strategies targeting hijacked cellular processes are less easily evaded by the virus than viral targets. If selective for viral functions, they can have a high therapeutic index.
Carin K. Ingemarsdotter, Sushmita Poddar, Sarah Mercier, Volker Patzel, Andrew M.L. Lever   +4 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Rapid Genome-wide Recruitment of RNA Polymerase II Drives Transcription, Splicing, and Translation Events during T Cell Responses

Cell Reports, 2017
Summary: Activation of immune cells results in rapid functional changes, but how such fast changes are accomplished remains enigmatic. By combining time courses of 4sU-seq, RNA-seq, ribosome profiling (RP), and RNA polymerase II (RNA Pol II) ChIP-seq ...
Kathrin Davari, Johannes Lichti, Christian Gallus, Franziska Greulich, N. Henriette Uhlenhaut, Matthias Heinig, Caroline C. Friedel, Elke Glasmacher   +7 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

RNA structure and splicing regulation [PDF]

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2019
The process of pre-mRNA splicing is fundamental to gene regulation in eukaryotes. Specific RNA sequences called introns, which do not code for amino acids, are removed during pre-mRNA splicing, and flanking sequences are spliced together as exons forming mRNAs that are exported to the cytosol.
Baralle, Francisco E., Singh, Ravindra, Stamm, Stefan   +2 more
openaire   +3 more sources

Transcriptomic and genomic identification of spliceosomal genes from Euglena gracilis

Acta Biochimica et Biophysica Sinica, 2023
Diverse splicing types in nuclear and chloroplast genes of protist Euglena gracilis have been recognized for decades. However, the splicing machinery responsible for processing nuclear precursor messenger RNA introns, including
Gao Pingwei, Zhong Yujie, Sun Chengfu
doaj   +1 more source

Cis‐regulatory and long noncoding RNA alterations in breast cancer – current insights, biomarker utility, and the critical need for functional validation

Molecular Oncology, EarlyView.
The noncoding region of the genome plays a key role in regulating gene expression, and mutations within these regions are capable of altering it. Researchers have identified multiple functional noncoding mutations associated with increased cancer risk in the genome of breast cancer patients.
Arnau Cuy Saqués, Aracele Martinez‐Mendez, John Crown, Alex Eustace   +3 more
wiley   +1 more source

Alternative RNA Splicing in Fatty Liver Disease

Frontiers in Endocrinology, 2021
Alternative RNA splicing is a process by which introns are removed and exons are assembled to construct different RNA transcript isoforms from a single pre-mRNA.
Panyisha Wu, Moya Zhang, Nicholas J. G. Webster, Nicholas J. G. Webster, Nicholas J. G. Webster   +4 more
doaj   +1 more source

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

Molecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice, Jose I. Lopez, Aitziber Ugalde‐Olano, Maite Zufiaurre, Ianire Astobiza, Natalia Martin‐Martin, Laura Bozal‐Basterra, Saioa Garcia‐Longarte, Amaia Zabala‐Letona, Sofia Rey, Aida Santos‐Martin, Miguel Unda, Ana Loizaga‐Iriarte, Mariona Graupera, Paolo Nuciforo, Arkaitz Carracedo, Isabel Mendizabal   +16 more
wiley   +1 more source