Results 81 to 90 of about 154,659 (331)

HIV-1: To Splice or Not to Splice, That Is the Question

Viruses, 2021
The transcription of the HIV-1 provirus results in only one type of transcript—full length genomic RNA. To make the mRNA transcripts for the accessory proteins Tat and Rev, the genomic RNA must completely splice.
Ann Emery, Ronald Swanstrom
doaj   +1 more source

A unified mechanism for intron and exon definition and back-splicing. [PDF]

, 2019
The molecular mechanisms of exon definition and back-splicing are fundamental unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy structures of the yeast spliceosomal E complex assembled on introns, providing a view of the ...
Cui, Yanxiang, Das, Rhiju, Espinosa, Sara, Hansen, Kirk C, Hill, Ryan C, Issaian, Aaron, Kappel, Kalli, Li, Xueni, Liu, Shiheng, Shi, Shasha, Zhang, Lingdi, Zhao, Rui, Zhou, Z Hong   +12 more
core  

Genetic regulators of stress-induced RNA mis-splicing in Caenorhabditis elegans [PDF]

, 2021
Splicing of pre-mRNA is an essential process for all eukaryotic dividing cells. Pre-mRNA splicing defects are implicated in numerous human diseases, including Alzheimer’s disease and cancer, however its cause is poorly understood.
Chomyshen, Samantha Corinne
core  

Structural insights into RNA splicing [PDF]

Current Opinion in Structural Biology, 2009
Intron splicing is a fundamental biological process whereby noncoding sequences are removed from precursor RNAs. Recent work has provided new insights into the structural features and reaction mechanisms of two introns that catalyze their own splicing from precursor RNA: the group I and II introns.
Kevin S. Keating, Navtej Toor, Anna Marie Pyle, Anna Marie Pyle   +3 more
openaire   +3 more sources

Molecular and functional profiling unravels targetable vulnerabilities in colorectal cancer

Molecular Oncology, EarlyView.
We used whole exome and RNA‐sequencing to profile divergent genomic and transcriptomic landscapes of microsatellite stable (MSS) and microsatellite instable (MSI) colorectal cancer. Alterations were classified using a computational score for integrative cancer variant annotation and prioritization.
Efstathios‐Iason Vlachavas, Konstantinos Voutetakis, Vivian Kosmidou, Spyridon Tsikalakis, Spyridon Roditis, Konstantinos Pateas, Ryangguk Kim, Kymberleigh Pagel, Stephan Wolf, Gregor Warsow, Antonia Dimitrakopoulou‐Strauss, Georgios N. Zografos, Alexander Pintzas, Johannes Betge, Olga Papadodima, Stefan Wiemann   +15 more
wiley   +1 more source

Splicing Factors Have an Essential Role in Prostate Cancer Progression and Androgen Receptor Signaling

Biomolecules, 2019
Although inhibition of the androgen–androgen receptor (AR) axis effectively represses the growth of prostate cancer, most of all cases eventually become castration-resistant prostate cancers (CRPCs).
Ken-ichi Takayama
doaj   +1 more source

Proteomic identification of heterogeneous nuclear ribonucleoprotein L as a novel component of SLM/Sam68 nuclear bodies [PDF]

, 2009
Background: Active pre-mRNA splicing occurs co-transcriptionally, and takes place throughout the nucleoplasm of eukaryotic cells. Splicing decisions are controlled by networks of nuclear RNA-binding proteins and their target sequences, sometimes in ...
Bindereif, A., Bourgeois, C.F., Clark, E.L., Dalgliesh, C., Elliott, D.J., Emami, K., Heiner, M., Leung, H.Y., Rajan, P., Robson, C.N., Stevenin, J.   +10 more
core   +7 more sources

Blood RNA analysis can increase clinical diagnostic rate and resolve variants of uncertain significance [PDF]

, 2020
Purpose Diagnosis of genetic disorders is hampered by large numbers of variants of uncertain significance (VUSs) identified through next-generation sequencing. Many such variants may disrupt normal RNA splicing.
Baralle, D., Bunyan, D.J., Cibin, P., Douglas, A.G.L., Ellard, S., Gunning, A., Kelly, H., Lord, J., Lye, J., Lyon, M., Seaby, E.G., Spiers-Fitzgerald, K., Thomas, N.S., Wai, H.A.   +13 more
core   +5 more sources

CircCCNB1 inhibits vasculogenic mimicry by sequestering NF90 to promote miR‐15b‐5p and miR‐7‐1‐3p processing in nasopharyngeal carcinoma

Molecular Oncology, EarlyView.
CircCCNB1 expression is down‐regulated in nasopharyngeal carcinoma (NPC); thus, less NF90 protein is bound to circCCNB1 and more binds to pri‐miRNAs, blocking their (pri‐miRNAs) binding to DGCR8 and inhibiting the processing and generation of miR‐15b‐5p/miR‐7‐1‐3p. Furthermore, decreased miR‐15b‐5p/miR‐7‐1‐3p promotes the expression of the target genes
Chunmei Fan, Fenghua Tan, Jie Wu, Zhaoyang Zeng, Wenjia Guo, He Huang, Wei Xiong   +6 more
wiley   +1 more source

Bayesian Reconstruction and Differential Testing of Excised mRNA [PDF]

arXiv, 2022
Characterizing the differential excision of mRNA is critical for understanding the functional complexity of a cell or tissue, from normal developmental processes to disease pathogenesis. Most transcript reconstruction methods infer full-length transcripts from high-throughput sequencing data.
arxiv