Results 211 to 220 of about 522,625 (254)
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Transcriptome-Wide Profiling of RNA Stability
2021Gene expression is controlled at multiple levels, including RNA transcription and turnover. But determining the relative contributions of RNA biogenesis and decay to the steady-state abundance of cellular transcripts remains challenging because conventional transcriptomics approaches do not provide the temporal resolution to derive the kinetic ...
Fasching, Nina +4 more
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Stabilizing RNA by the Sonochemical Formation of RNA Nanospheres
Small, 2011AbstractBiological macromolecules, including DNA, RNA, and proteins, have intrinsic features that make them potential building blocks for the bottom‐up fabrication of nanodevices. Unlike DNA, RNA is a more versatile molecule whose range in the cell is from 21 to thousands of nucleotides and is usually folded into stem and loop structures. RNA is unique
Ulyana, Shimanovich +5 more
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Stability of the messenger RNA-transfer RNA-ribosome complex
Journal of Molecular Biology, 1968Abstract The thermal stability of the mRNA-aminoacyl-tRNA-ribosome complex and each of its individual components was examined. A temperature-dependent, reversible dissociation of the mRNA-tRNA-ribosome complex has been demonstrated. By analogy with polynucleotide melting curves, the dissociation of the mRNA-tRNA-ribosome complex is considered as a ...
C S, McLaughlin +4 more
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Control of messenger RNA stability
Immunology Today, 1987Gene expression can be controlled at many levels. Changes in the rate of transcription of a gene, the rate of nuclear processing of the transcript (capping, termination, polyadenylation and splicing) and the rate of transport of the processed transc,ipt from the nucleus to the cytoplasm can contribute to changes in the levels of a particular mRNA. Once
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Determinants of messenger RNA stability
Cell, 1987The steady-state levels of functional mRNAs are determined in part by their rates of decay in the cytoplasm. Hence, this process plays an important role in the control of gene expression. Individual mRNA species differ widely with respect to metabolic stability.
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RNA Preservation and Stabilization
Biochemistry & Physiology: Open Access, 2014RNA quality and integrity is a prominent issue in gene expression analysis which emerged as a critical tool in life science researches, drug discovery and optimization of bioproduction. Handling and preservation methods including formalin fixation, flash freezing and chemical preservatives (sulfate solution and TRIzol) and commercial compounds ...
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Stabilization of RNA Encapsulated in Silk
ACS Biomaterials Science & Engineering, 2018The use of mRNA and miRNA as diagnostic parameters and therapeutic agents has drawn wide interest both clinically and in research. However, RNA is a labile molecule, which requires strict storage conditions, often including cold temperatures or dry environments, in order to preserve RNA integrity.
Jiuyang He +5 more
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2006
The potential importance of RNA stability to the regulation of chloroplast gene expression is documented by recent work on the molecular aspects of chloroplast biogenesis. In Chlamydomonas reinhardtii, two lines of evidence support the idea that gene-specific regulated RNA decay plays a crucial role in determining the different steady-state levels of ...
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The potential importance of RNA stability to the regulation of chloroplast gene expression is documented by recent work on the molecular aspects of chloroplast biogenesis. In Chlamydomonas reinhardtii, two lines of evidence support the idea that gene-specific regulated RNA decay plays a crucial role in determining the different steady-state levels of ...
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Stability of nucleolar 45-S RNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967Abstract With respect to sedimentation, nucleolar 45-S RNA was stable for 48 h at room temperature in 0.05 M sodium acetate buffer at pH 5.1 and in Tris buffer at pH 9.0 for 2 h. No change in the sedimentation pattern occurred after dialysis, incubation with EDTA or incubation with proteolytic enzymes.
K, Higashi, H, Busch
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1998
The regulation of RNA stability represents one of the key processes determining the level of chloroplast gene expression [1–3]. Several lines of evidence support this idea: 1) Changes in transcription rates alone cannot account for the different transcript levels of plastid genes detected in light versus dark grown higher plants [4, 5].
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The regulation of RNA stability represents one of the key processes determining the level of chloroplast gene expression [1–3]. Several lines of evidence support this idea: 1) Changes in transcription rates alone cannot account for the different transcript levels of plastid genes detected in light versus dark grown higher plants [4, 5].
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