Results 221 to 230 of about 544,829 (251)
Some of the next articles are maybe not open access.
RNA Preservation and Stabilization
Biochemistry & Physiology: Open Access, 2014RNA quality and integrity is a prominent issue in gene expression analysis which emerged as a critical tool in life science researches, drug discovery and optimization of bioproduction. Handling and preservation methods including formalin fixation, flash freezing and chemical preservatives (sulfate solution and TRIzol) and commercial compounds ...
openaire +1 more source
RNA Processing and RNA Stability in Plant Mitochondria
2010In angiosperms, mitochondrial genomes encode about 60 genes. The corresponding gene products are all directly or indirectly involved in oxidative phosphorylation indicating their vital function. To realize the mitochondrial genetic information, mitochondria maintain comprehensive genetic systems.
Stefan Binder +2 more
openaire +1 more source
Stability of nucleolar 45-S RNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967Abstract With respect to sedimentation, nucleolar 45-S RNA was stable for 48 h at room temperature in 0.05 M sodium acetate buffer at pH 5.1 and in Tris buffer at pH 9.0 for 2 h. No change in the sedimentation pattern occurred after dialysis, incubation with EDTA or incubation with proteolytic enzymes.
K, Higashi, H, Busch
openaire +2 more sources
On the role of magnesium ions in RNA stability
Biopolymers, 1998Divalent cations, like magnesium, are crucial for the structural integrity and biological activity of RNA. In this article, we present a picture of how magnesium stabilizes a particular folded form of RNA. The overall stabilization of RNA by Mg2+ is given by the free energy of transferring RNA from a reference univalent salt solution to a mixed salt ...
V K, Misra, D E, Draper
openaire +2 more sources
Stability of nuclear RNA in mammalian cells
Journal of Molecular Biology, 1974Abstract The kinetics of entry of [3H]adenosine into ATP, cellular RNA, and nuclear RNA of mouse L cells were determined and analyzed. A molar accumulation curve for RNA was estimated from the specific radioactivities of RNA and ATP; this curve was resolved graphically into stable and unstable components.
B P, Brandhorst, E H, McConkey
openaire +2 more sources
1998
The regulation of RNA stability represents one of the key processes determining the level of chloroplast gene expression [1–3]. Several lines of evidence support this idea: 1) Changes in transcription rates alone cannot account for the different transcript levels of plastid genes detected in light versus dark grown higher plants [4, 5].
openaire +1 more source
The regulation of RNA stability represents one of the key processes determining the level of chloroplast gene expression [1–3]. Several lines of evidence support this idea: 1) Changes in transcription rates alone cannot account for the different transcript levels of plastid genes detected in light versus dark grown higher plants [4, 5].
openaire +1 more source
Poly(A)-assisted RNA decay and modulators of RNA stability.
Progress in molecular biology and translational science, 2009In Escherichia coli, RNA degradation is orchestrated by the degradosome with the assistance of complementary pathways and regulatory cofactors described in this chapter. They control the stability of each transcript and regulate the expression of many genes involved in environmental adaptation.
Ph., Régnier, Hajnsdorf, E.
openaire +2 more sources
2006
The potential importance of RNA stability to the regulation of chloroplast gene expression is documented by recent work on the molecular aspects of chloroplast biogenesis. In Chlamydomonas reinhardtii, two lines of evidence support the idea that gene-specific regulated RNA decay plays a crucial role in determining the different steady-state levels of ...
openaire +1 more source
The potential importance of RNA stability to the regulation of chloroplast gene expression is documented by recent work on the molecular aspects of chloroplast biogenesis. In Chlamydomonas reinhardtii, two lines of evidence support the idea that gene-specific regulated RNA decay plays a crucial role in determining the different steady-state levels of ...
openaire +1 more source
Evaluating the Stability of mRNAs and Noncoding RNAs
2016Changes in RNA stability have an important impact in the gene expression regulation. Different methods based on the transcription blockage with RNA polymerase inhibitors or metabolic labeling of newly synthesized RNAs have been developed to evaluate RNA decay rates in cultured cell.
Ana Carolina, Ayupe, Eduardo M, Reis
openaire +2 more sources