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Nuclear actin and transport of RNA

Cell Biology International Reports, 1987
The role of nuclear actin filaments in the RNA transport was investigated. Mouse lymphoma cells, L5178Y, were labeled for 20 min with 3H-uridine, and the isolated nuclei were incubated in a medium consisting of 0.25 M sucrose, 10 mM Tris-HCl (pH 7.5), 2 mM CaCl2, 1 mM ATP and 1mM PMSF.
H, Ueyama, H, Nakayasu, K, Ueda
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RNA nucleocytoplasmic transport

Seminars in Cell Biology, 1992
The transport of RNAs from the nucleus to the cytoplasm is an obligatory step in gene expression and may also be a target for regulation. The cellular machinery has the capacity to export a myriad of RNA transcripts, which differ significantly in sequence and structure. Recent work is providing the first glimpses into how RNA export occurs.
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THE DIRECT MEASUREMENT OF THE AXOPLASMIC TRANSPORT OF INDIVIDUAL RNA SPECIES: TRANSFER BUT NOT RIBOSOMAL RNA IS TRANSPORTED

Journal of Neurochemistry, 1979
AbstractAnalysis of chick retinal and tectal RNA revealed that in addition to the major cytoplasmic RNAs (rRNA and tRNA), a number of the small mol wt nuclear RNAs (snRNAs) can also be detected. Subfractionation data indicated that one of these molecules, DD′, is of at least 95% nuclear location within the retina.
P W, Gunning   +5 more
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Intracellular RNA sorting, transport and localization

Nature Structural & Molecular Biology, 2005
RNA localization and translational control are crucial for cellular fine-tuning of gene expression in space and time. A recent meeting in Tucson, Arizona pointed out mechanisms conserved across different species and cell types that contribute to the establishment of cell polarity and cell migration.
Ralf-Peter, Jansen, Michael, Kiebler
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Energy utilization and RNA transport: their interdependence

Biochemistry, 1978
The interdependence of RNA transport and the metabolism of nucleotide additives was investigated. Rat-liver RNA was radioactively labeled in vivo for 45 min before isolation of liver nuclei, and the concentration dependence of RNA transport on nucleotide additives was determined.
G A, Clawson   +3 more
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Nucleocytoplasmic RNA Transport

1984
During the past decade, it has become evident that, in eukaryotes, RNA molecules undergo a complex series of chemical modifications after transcription and before translation and that some of these modifications coincide with alterations in subcellular distribution.
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Characterization of a messenger RNA transport protein

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1983
A cytoplasmic protein which facilitates the energy-dependent transport of mRNA from isolated nuclei to a specified medium has been further characterized, since it could have relevance to the mechanism of mRNA nucleo-cytoplasmic transport in vivo. This protein is now shown, by cDNA hybridization analysis using appropriate recombinant probes, to be ...
R B, Moffett, T E, Webb
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RNA transport: Delivering the message

Nature Plants, 2015
Messenger RNAs are translocated between plant shoots and roots in patterns that reflect directionality, environmental responsiveness, and organ targeting.
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Messenger RNA transport and localization

Current Opinion in Cell Biology, 1990
SpeciEcity can be imparted to subcellular regions by the setting and subsequent localization of proteins and RNA Although the study of RNA trafficking is in its infancy, the process has been documented already in large germ cells and in asymmetric differentiated cells where visualization of preferential RNA compartmentalization is feasible.
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Actinomycin D and RNA transport

Nature, 1974
ACTINOMYCIN D, a potent inhibitor of RNA chain propagation of DNA-directed RNA transcription1–3, has been widely used for studies of turnover and transport of RNA in a variety of cell systems, to try to demonstrate that nuclear RNA labelled before the addition of actinomycin D is transported to the cytoplasm.
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