The metabolism of aged seeds. The ribonucleolytic activity of rye grain embryos of different ages
Acta Societatis Botanicorum Poloniae, 2014 Winter rye grain, harvested in various years and exhibiting clearly differing viability. was studied. Higher total ribonucleolytic activity was found in the post-ribosomal supernatant than in the ribosomes themselves.
Kazimierz Zalewski
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Chemical evolution of an autonomous DNAzyme with allele-specific gene silencing activity
Nature Communications, 2023 Low activity has been the primary obstacle impeding the use of DNA enzymes (DNAzymes) as gene silencing agents in clinical applications. Here we describe the chemical evolution of a DNAzyme with strong catalytic activity under near physiological ...
Kim Nguyen +2 more
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A functional RNase P protein subunit of bacterial origin in some eukaryotes [PDF]
, 2011 RNase P catalyzes 5′-maturation of tRNAs. While bacterial RNase P comprises an RNA catalyst and a protein cofactor, the eukaryotic (nuclear) variant contains an RNA and up to ten proteins, all unrelated to the bacterial protein.
Bernal Bayard, Pilar +5 more
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Revista do Instituto de Medicina Tropical de São Paulo, 1995 Leishmania braziliensis is a causative agent of American Cutaneous Leishmaniasis (ACL). The 034-JCG strain, isolated from a patient from the northern region of Paraná State, Brazil, was cultivated in Blood Agar Base medium, lyophilized and submitted to ...
T. G. V. Silveira, C Kemmelmeier
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The occupancy of two distinct conformations by active-site histidine-119 in crystals of ribonuclease is modulated by pH [PDF]
, 1994 Structures of a semisynthetic RNase have been obtained to a resolution of 2.0 Å at pH values of 5.2, 6.5, 7.5, and 8.8, respectively. The principle structural transformation occurring over this pH range is the conversion of the side chain of active site ...
J. de Mel, V.Srini +3 more
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Atypical protein disulfide isomerases (PDI): Comparison of the molecular and catalytic properties of poplar PDI-A and PDI-M with PDI-L1A. [PDF]
PLoS ONE, 2017 Protein disulfide isomerases are overwhelmingly multi-modular redox catalysts able to perform the formation, reduction or isomerisation of disulfide bonds. We present here the biochemical characterization of three different poplar PDI isoforms.
Benjamin Selles +4 more
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Circulating microRNAs -The potential biomarkers for early diagnosis of osteoarthritis
Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ, 2020 Osteoarthritis (OA) is the most common form of arthritis and is one of the main causes of joint pain, impaired mobility and physical disability. Early diagnosis of OA is an important factor for effective treatment. However, current diagnostic methods are
Hồ Thị Bích Phương +2 more
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RNA-binding protein immunoprecipitation as a tool to investigate plant miRNA processing interference by regulatory proteins of diverse origin [PDF]
, 2018 Background: Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways.
Garcia, Maria Laura +2 more
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Biomolecules, 2023 Research into molecular mechanisms of self-incompatibility (SI) in plants can be observed in representatives of various families, including Solanaceae. Earlier studies of the mechanisms of S-RNase-based SI in petunia (Petunia hybrida E.
Ekaterina Zakharova +4 more
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Cellular delivery of quantum dot-bound hybridization probe for detection of intracellular pre-microRNA using chitosan/poly(γ-glutamic acid) complex as a carrier. [PDF]
PLoS ONE, 2013 A quantum dot (QD)-bound hybridization probe was designed for detection of intracellular pre-miRNA using chitosan (CS)/poly(γ-glutamic acid) (γ-PGA) complex as a gene vector. The probe was prepared by assembling thiolated RNA to gold nanoparticle (Au NP)
Yao Geng +4 more
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