Results 231 to 240 of about 321,082 (269)

Co-transcriptional modifications of 2'-hydroxyls on synthetic mRNA enhance ribonuclease resistance and lipid nanoparticle thermostability. [PDF]

Mol Ther Nucleic Acids
Yamada K, Sung YC, Lee TY, Siegwart DJ.
europepmc   +1 more source

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Harnessing toxin-mediated ribosome stalling as a complementary tool to annotate bacterial ORFs. [PDF]

Nucleic Acids Res
Troian EA, Barth VC, Chauhan U, Zheng H, Zhao C, Zeng J, Husson RN, Woychik NA.   +7 more
europepmc   +1 more source

TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

Advanced Science, EarlyView.
The TopCas platform utilizes an engineered DNA‐RNA chimeric circular crRNA to establish topology‐gated control over CRISPR‐Cas12a activity, enabling target‐triggered self‐catalytic amplification (preamplification‐free). This system seamlessly integrates highly sensitive molecular diagnostics with conditionally controlled gene editing, demonstrating ...
Shun Zhang, Wen Sun, Ting Xiao, Yali Wang, Xianlan Wu, Huiyou Chen, Ming Chen, Jun Zhang   +7 more
wiley   +1 more source

Enzymatic Basis for the Oxidative Branch of Aromatic Amino Acid Fermentation Leading to p‐cresol Formation

Advanced Science, EarlyView.
In anaerobic bacteria, tyrosine serves as an electron donor in the oxidative Stickland fermentation branch, generating p‐hydroxyphenylacetate, ATP and reduced ferredoxin for decarboxylation into p‐cresol. ABSTRACT The phenolic metabolite p‐cresol is a byproduct of tyrosine fermentation by certain strictly anaerobic bacteria, including the human gut ...
Li Jiang, Yifeng Wei, Xumei Liu, Dazhi Liu, Zhenyu Liu, Yang Tong, Jinyu Yin, Ankanahalli N. Nanjaraj Urs, Meining Xing, Mark A. Harrison, Chuyuan Zhang, Sheng Yang, Yunzi Luo, Ee Lui Ang, Huimin Zhao, Yan Zhang   +15 more
wiley   +1 more source

Prophylactic self-assembled nucleic acid hydrogel targeting retinal ganglion cell cuproptosis and microglial neuroinflammation for retinal ischemia-reperfusion injury. [PDF]

Bioact Mater
Liu S, Wang Z, Wu Y, Ju Y, Tang Z, Zhang D, Chen X, Wang N, Song M, Gu C, Liu Y, Guo W, Zhang J, Xiao C, Lu L, Zhang C, Ni N, Gu P.   +17 more
europepmc   +1 more source

Cytoplasmic mRNA decay by the antiviral nuclease RNase L promotes transcriptional repression. [PDF]

Cell Rep
Mao X, Tokamov S, Pahmeier F, Lari A, Xu J, Harris E, Glaunsinger B.   +6 more
europepmc   +1 more source

The non-tandem CCCH zinc finger protein AtC3H26 modulates salt stress response and phosphate starvation response through its ribonuclease activity in Arabidopsis. [PDF]

BMC Plant Biol
Seok HY, Jang Y, Lee SY, Kim D, Nguyen LV, Moon YH.   +5 more
europepmc   +1 more source

RNase MRP subunit composition and role in 40S ribosome biogenesis. [PDF]

Nat Struct Mol Biol
Smith EM, Ly J, Haug S, Cheeseman IM.
europepmc   +1 more source

A Small Cationic Probe for Accurate, Punctate Discovery of RNA Tertiary Structure. [PDF]

JACS Au
Ehrhardt JE, Qiu DY, Jin S, Boerneke MA, Aufgebauer CJ, Horner SM, Weeks KM.   +6 more
europepmc   +1 more source