Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Dual Regulation of the Rice Yield Traits and Stress Resilience by <i>OsHSP20</i>: Insights from Integrative Biochemical and Transcriptomic Analyses. [PDF]
Liao Y +14 more
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
TaMIP1 gene breaks the dilemma of coordinating drought resistance and high yield in wheat. [PDF]
Xu Z, Zhang R, Zhang N.
europepmc +1 more source
Combining non-microbial biostimulants and different application methods affects yield, antioxidant activity, nutritional and functional value of pea and arugula microgreens. [PDF]
Consentino BB +9 more
europepmc +1 more source
Alternate partial root zone subsurface drip irrigation increases alfalfa water production function by increasing hay yield, rather than actual crop evapotranspiration. [PDF]
Wang Y, Li Y, Zhang Q, Gao K, Su D.
europepmc +1 more source
Effects of Foliar-Applied PAA@Mn<sub>3</sub>O<sub>4</sub> Nanoparticles, Methyl Jasmonate, and γ-Aminobutyric Acid on Growth and Yield Performance of Sugar Beet (<i>Beta vulgaris</i> L.) Under Salt-Affected Conditions. [PDF]
Zhang Z +5 more
europepmc +1 more source
Beyond Nitrogen Fixation: Multifunctional Roles of Common Bean in Organic Agroecosystems and Ecosystem-Oriented Breeding Strategies. [PDF]
Avasiloaiei DI +5 more
europepmc +1 more source

