Results 221 to 230 of about 2,554,734 (253)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Dual Regulation of the Rice Yield Traits and Stress Resilience by <i>OsHSP20</i>: Insights from Integrative Biochemical and Transcriptomic Analyses. [PDF]

open access: yesInt J Mol Sci
Liao Y   +14 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Combining non-microbial biostimulants and different application methods affects yield, antioxidant activity, nutritional and functional value of pea and arugula microgreens. [PDF]

open access: yesFront Plant Sci
Consentino BB   +9 more
europepmc   +1 more source

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