Results 91 to 100 of about 2,492,535 (278)
HSP70 governs permeability and mechanotransduction in primary human endothelial cells
FEBS Open Bio, EarlyView.HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez +5 more
wiley +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
, 2012 Two classes of conservative, linear, optical rotary effects (optical activity and Faraday rotation) are distinguished by their behavior under time reversal.
Andrews, James H. +2 more
core +1 more source
AgricultureThe destructive impact of invasive apple snail (Pomacea canaliculata) on young rice seedlings has garnered global attention, particularly in warm regions where rice production occurs.
Senlin Guan +3 more
doaj +1 more source
Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node
FEBS Open Bio, EarlyView.The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner +6 more
wiley +1 more source
Three-dimensional coating and rimming flow: a ring of fluid on a rotating horizontal cylinder [PDF]
, 2012 The steady three-dimensional flow of a thin, slowly varying ring of Newtonian fluid on either the outside or the inside of a uniformly rotating large horizontal cylinder is investigated.
Duffy, B. R. +2 more
core
A Cell Migration Tracking Tool Supports Coupling of Tissue Rotation to Elongation
Cell Reports, 2017 Cell migration is indispensable to morphogenesis and homeostasis. Live imaging allows mechanistic insights, but long-term observation can alter normal biology, and tools to track movements in vivo without perturbation are lacking.
Dong-Yuan Chen +2 more
doaj +1 more source
SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Australasian Orthodontic Journal, 1990 The presence of supernumerary teeth in the premaxillary region may cause a variety of pathological effects. This study was carried out to determine the various aspects of supernumerary teeth in the premaxillary region, particularly the effects of the ...
Nik-Hussein Nik Noriah
doaj +1 more source
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source