Results 91 to 100 of about 1,093,749 (248)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function

FEBS Open Bio, EarlyView.
Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley, Luke W. Molesworth, Nethma H. Waduge, Fahimeh Eskandari, Peter R. Boag, Gregory M. Davis   +5 more
wiley   +1 more source

Evidence map of crop diversification strategies at the global scale

Environmental Research Letters, 2019
The diversification of cropping systems encompasses different strategies that may help maintain or enhance the sustainability of agriculture. Thousands of experiments have been carried out around the world since almost five decades to evaluate and ...
Damien Beillouin, Tamara Ben-Ari, David Makowski   +2 more
doaj   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Crop rotation phase has a greater impact on soil biology than crop rotation diversity

The effect of plant diversity on the belowground soil food web remains poorly understood. In this study the soil microbial community structure and biomass, and the abundance of microfauna, mesofauna, and macrofauna were assessed at three levels of crop ...
Thomas, Sion, Struijk, M., Leigh, Sam, Mortimer, Simon, Ashwood, F., Degani, Erika, Ashwood, Frank, Sizmur, Tom, Leigh, S. G., Whitmore, A. P., Thomas, S., Sizmur, T., Clark, Suzanne J., Mortimer, S. R., Bowen, Emma, Bowen, E., Struijk, Marijke, Degani, E., Whitmore, Andrew P., Clark, S. J.   +19 more
core   +1 more source

Deteksi Kesegaran Daging Sapi Menggunakan Augmentasi Data Mosaic pada Model YOLOv5sM

JRST: Jurnal Riset Sains dan Teknologi
Deteksi kesegaran daging sapi secara otomatis sangat penting dalam mendukung kualitas bahan pangan, terutama dalam mencegah konsumsi daging yang sudah tidak layak dan berisiko terhadap kesehatan.
Anton Yudhana, Esi Putri Silmina, Sunardi Sunardi   +2 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Endogenous Bid Rotation in Repeated Auctions [PDF]

I study collusion between two bidders in a general symmetric IPV repeated auction, without communication, side transfers, or public randomization. I construct a collusive scheme, endogenous bid rotation, that generates a payoff larger than the bid ...
Rachmilevitch, Shiran
core