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A method for determination of hexokinase activity by RP-HPLC

Wuhan University Journal of Natural Sciences, 2011
A method was established to determine the activity of hexokinase by reverse-phase high-performance liquid chromatography (RP-HPLC). The activity of hexokinase was determined by detecting the amount of ADP in the reaction that converts glucose into glucose-6-phosphate. The separation degree of ATP and ADP was as good as 3.46 (separation degree>1.5).
Yuxia Guan, Jiaxian Sun, Jing Wang
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Method Development and Validation of Lorlatinib by RP-HPLC

Chinese Journal of Applied Physiology
A simple, Accurate, precise method was developed for the estimation of the Lorlatinib in API form and Marketed pharmaceutical dosage form by RP-HPLC. Chromatogram was run through Hypersil C18 (4.6mm×150mm, 5µm) Particle size Column and Mobile phase containing Methanol and Water taken in the ratio of 25: 75% v/v was pumped through column at a flow rate ...
Santhosh, Illendula, Shailesh, Sharma
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Separation and Identification of Labile Polyacetylenes by RP-HPLC

Journal of Liquid Chromatography, 1989
Abstract Traditional methods of isolation and identification of labile polyacetylenes often lead to substantial loss of material. Reverse-phase HPLC coupled with a diode array detector offers a fast and convenient way to isolate and identify polyacetylenes from plant sources.
Sue C. Witt, Kenneth L. Stevens
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Razdvajanje bjelančevina žitarica RP-HPLC metodom

2005
Za analizu bjelančevina žitarica, zbog njihove heterogenosti, slabe topljivosti i tendencije polimerizaciji razvijaju se i primjenjuju nove metode kojima se postiže bolja rezolucija i ponovljivost pri razdvajanju. Širu primjenu našle su elektroforetske i kromatografske metode.
Horvat, Daniela   +3 more
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Determination and Method Validation of Metamitron in Soil by RP-HPLC

Bulletin of Environmental Contamination and Toxicology, 2013
A simple, rapid and economical method was developed and validated for the determination of metamitron using UV detector with RP-HPLC. Study of metamitron in soil was carried out. The compound was extracted from soil by methanol and clean-up was done on C-18 SPE column.
Satyendra Kumar   +2 more
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Proteolytic Cleavage in RP-HPLC Solvents

1987
Successful degradation of the very resistant human complement component C3 (Mr 187,500) with trypsin in the presence of 2-propanol (1) has prompted us to examine the effects of the lower alcohols and acetonitrile during degradation with some commonly used endoproteases. The benefits might be threefold.
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Phenomenological approach to the mechanism of retention in RP HPLC

Chromatographia, 2002
The retention process in reversed-phase liquid chromatography (RPLC) and the mechanism behind it can best be followed by a phenomenological description of molecular interactions. This can be accomplished by using the linear solvation-energy relationship (LSER).
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VALIDATED RP-HPLC METHOD FOR DETERMINATION OF DACOMITINIB IN PURE FORM AND TABLET DOSAGE FORM BY USING RP-HPLC

A new simple, selective, rapid, precise RP- HPLC method is described for the determination of Dacomitinib in bulk and marketed pharmaceutical dosage form. Chromatography was carried on a Symmetry C18 (4.6×150mm) 5µm column using a mixture of Methanol: Phosphate Buffer (pH-4.6) (25:75) V/V as the mobile phase at a flow rate of 1.0mL/min with detection ...
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