Results 41 to 50 of about 582,607 (300)

Tax4Fun2: prediction of habitat-specific functional profiles and functional redundancy based on 16S rRNA gene sequences

open access: yesEnvironmental Microbiome, 2020
Sequencing of 16S rRNA genes has become a powerful technique to study microbial communities and their responses towards changing environmental conditions in various ecosystems.
F. Wemheuer   +6 more
semanticscholar   +1 more source

Cloning and sequencing of the complicated rDNA gene family of Bos taurus

open access: yesCzech Journal of Animal Science, 2006
The rDNA genes coding for ribosomal RNA (rRNA) in animals are repeat sequences with high GC content and complicated structure. Based on the sequences of human ribosomal DNA repeat unit and transcription unit and the long and accurate PCR method with LA ...
D. Tang   +5 more
doaj   +1 more source

Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules. [PDF]

open access: yesPLoS ONE, 2016
PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes.
James E McDonald   +8 more
doaj   +1 more source

Compilation of 5S rRNA and 5S rRNA gene sequences

open access: yesNucleic Acids Research, 1988
The BERLIN RNA DATABANK as of December 31, 1987, contains a total of 509 sequences of 5S rRNAs or their genes, which is an increase of 45% over the last (1986) compilation (1). It covers sequences from 38 archaebacteria, 184 eubacteria, 14 plastids, 4 mitochondria, 258 eukaryotes and 11 eukaryotic pseudogenes. The BERLIN RNA DATABANK uses the format of
J, Wolters, V A, Erdmann
openaire   +3 more sources

PHYLOGENETIC DIVERSITY OF BACTERIAL POPULATION IN RUMEN

open access: yesActa Agriculturae Slovenica, 1998
In order to investigate the microbial diversity in rumen, bacterial 16S rRNA genes were amplified from total microbial DNA isolated from rumen fluid. The amplificated genes were incorporated into pBluescript SK II vector and electroporated into E.coli ...
Andreja RAMŠAK, G. AVGUŠTIN
doaj   +1 more source

Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.

open access: yesGenome Research, 2011
Bacterial diversity among environmental samples is commonly assessed with PCR-amplified 16S rRNA gene (16S) sequences. Perceived diversity, however, can be influenced by sample preparation, primer selection, and formation of chimeric 16S amplification ...
B. Haas   +14 more
semanticscholar   +1 more source

Comparing bioinformatic pipelines for microbial 16S rRNA amplicon sequencing

open access: yesPLoS ONE, 2020
Microbial amplicon sequencing studies are an important tool in biological and biomedical research. Widespread 16S rRNA gene microbial surveys have shed light on the structure of many ecosystems inhabited by bacteria, including the human body.
A. Prodan   +5 more
semanticscholar   +1 more source

Complete mitochondrial genome of Saiga tatarica (Ruminantia; Pecora; Bovidae) isolate Wuwei in China

open access: yesMitochondrial DNA. Part B. Resources, 2017
This report described the complete mitochondrial genome of the Saiga antelope, Saiga tatarica, from the Gansu Endangered Animal Research Center (GEARC) in Gansu Province, China.
Xin Ding   +7 more
doaj   +1 more source

Diversity of 23S rRNA genes within individual prokaryotic genomes. [PDF]

open access: yesPLoS ONE, 2009
The concept of ribosomal constraints on rRNA genes is deduced primarily based on the comparison of consensus rRNA sequences between closely related species, but recent advances in whole-genome sequencing allow evaluation of this concept within organisms ...
Anna Pei   +6 more
doaj   +1 more source

Long-read MinION™ sequencing of 16S and 16S-ITS-23S rRNA genes provides species-level resolution of Lactobacillaceae in mixed communities

open access: yesFrontiers in Microbiology, 2023
The Lactobacillaceae are lactic acid bacteria harnessed to deliver important outcomes across numerous industries, and their unambiguous, species-level identification from mixed community environments is an important endeavor.
Sandra A. Olivier   +9 more
doaj   +1 more source

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