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Low-Cost Arduino Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) for Sensitive Nucleic Acid Detection. [PDF]
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Detection of Japanese yam mosaic virus by RT-LAMP
Archives of Virology, 2003Arapid and simple procedure is described to detect the genomic RNA molecule of Japanese yam mosaic potyvirus (JYMV). This method, named RT-LAMP, allows direct detection of RNA from infected plants without careful RNA extraction, rapid thermal cycling and gel electrophoresis.
S, Fukuta +6 more
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Establishment of RT-LAMP Detection Method for Bluetongue Virus
2018 9th International Conference on Information Technology in Medicine and Education (ITME), 2018In order to establish the RT-LAMP detectionmethod of bluetongue virus (BTV), 4 specific LAMP primers were designed and synthesized by using the conservative sequence of the BTV VP7 genome published by GenBank. Through the synthesis of BTV artificial genes, the optimization of the reaction conditions of LAMP, the LAMP sensitivity experiment of BTV and ...
Han Zhang +5 more
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Evaluation of RT-LAMP Assay for Rapid Detection of SARS-CoV-2
Laboratory Medicine, 2022Abstract Objective To evaluate the accuracy of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in community or primary-care settings ...
Ya-Ping, Li +7 more
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The detection and identification of saliva in forensic samples by RT-LAMP
Forensic Science, Medicine and Pathology, 2018We report on a novel method for saliva identification by reverse transcription-loop-mediated isothermal amplification (RT-LAMP). In our previous report, real-time RT-LAMP was used for blood identification by using HBB detection as a model but in this advanced study, this method was refined for the identification of the more challenging body fluid of ...
Li-Chin Tsai +7 more
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Fast real‐time detection of Potato spindle tuber viroid by RT‐LAMP
Plant Pathology, 2012This paper reports the development of a single tube, real‐time, reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) assay for detecting Potato spindle tuber viroid (PSTVd), one of the quarantine pathogens of potato in Europe and North America. The method enables detection of a broad range of PSTVd isolates, and is about 10 times more
Lenarčič, R. +3 more
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Fast and visual RT-LAMP assay for detection of oropouche virus
European Journal of Clinical Microbiology & Infectious DiseasesThe epidemic potential of Oropouche virus (OROV) highlights the urgent need for rapid and accessible diagnostic methods to improve testing capacity and reduce result turnaround times, especially in tropical regions where OROV prevalence is rising.To develop and validate a rapid, cost-effective diagnostic assay for OROV detection suitable for use in ...
Livia do Carmo Silva +7 more
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ND-UGA Tampon & RT-LAMP SARS-CoV-2 WBE
2021Data used to calculate sensitivity, specificity, limit of detection for RT-LAMP compared to RT-ddPCR/qPCR; data used to optimize tampon swab and RT-LAMP optimized ...
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Single-cell RT-LAMP mRNA detection by integrated droplet sorting and merging
Lab on a Chip, 2019We present a droplet-based microfluidic platform that permits seamless on-chip droplet sorting and merging, which enables completing multi-step reaction assays within a short time, and demonstrate detection of specific single-cell mRNA expressions.
Meng Ting Chung +2 more
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