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Development and validation of RT-LAMP for detecting yellow fever virus in non-human primates samples from Brazil. [PDF]
Sci RepCardoso SF +8 more
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Development and evaluation of a lyophilization protocol for colorimetric RT-LAMP diagnostic assay for COVID-19. [PDF]
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The detection and identification of saliva in forensic samples by RT-LAMP
Forensic Science, Medicine and Pathology, 2018We report on a novel method for saliva identification by reverse transcription-loop-mediated isothermal amplification (RT-LAMP). In our previous report, real-time RT-LAMP was used for blood identification by using HBB detection as a model but in this advanced study, this method was refined for the identification of the more challenging body fluid of ...
Li-Chin Tsai +7 more
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Detection of Japanese yam mosaic virus by RT-LAMP
Archives of Virology, 2003Arapid and simple procedure is described to detect the genomic RNA molecule of Japanese yam mosaic potyvirus (JYMV). This method, named RT-LAMP, allows direct detection of RNA from infected plants without careful RNA extraction, rapid thermal cycling and gel electrophoresis.
S, Fukuta +6 more
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Development of an optimized RT-LAMP test for the detection of SARS-CoV-2
Biologicals, 2023SARS-COV-2 is the causative agent of an acute respiratory syndrome called Coronavirus disease 2019 (COVID-19) with a varying mortality rate from 2019 to 2022. There are several measures for control and prevention of Covid-19 including using mask, vaccine injections, as well as screening the potential cases.
Navid, Momenifar +3 more
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Evaluation of RT-LAMP Assay for Rapid Detection of SARS-CoV-2
Laboratory Medicine, 2022Abstract Objective To evaluate the accuracy of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in community or primary-care settings ...
Ya-Ping, Li +7 more
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The detection of ACLSV and ASPV in pear plants by RT-LAMP assays
Journal of Virological Methods, 2018A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of Apple chlorotic leaf spot virus (ACLSV) and Apple stem pitting virus (ASPV), two important viruses frequently occurring in pear trees. A set of four RT-LAMP primers designed based on the highly conserved region of each CP gene of the two ...
Yongcan, Lu +3 more
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Establishment of RT-LAMP Detection Method for Bluetongue Virus
2018 9th International Conference on Information Technology in Medicine and Education (ITME), 2018In order to establish the RT-LAMP detectionmethod of bluetongue virus (BTV), 4 specific LAMP primers were designed and synthesized by using the conservative sequence of the BTV VP7 genome published by GenBank. Through the synthesis of BTV artificial genes, the optimization of the reaction conditions of LAMP, the LAMP sensitivity experiment of BTV and ...
Han Zhang +5 more
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Saliva identification by RT-LAMP integrated with CRISPR-Cas and LFA
Forensic Science, Medicine and PathologySaliva is a frequently encountered body fluid at crime scenes, however currently there are no definite means to rapidly identify a body fluid as being saliva. In this study, a novel detection method for saliva using a modified Loop-mediated Isothermal Amplification (LAMP) integrated with CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic ...
Chih-Wen Su +6 more
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Fast and visual RT-LAMP assay for detection of oropouche virus
European Journal of Clinical Microbiology & Infectious DiseasesThe epidemic potential of Oropouche virus (OROV) highlights the urgent need for rapid and accessible diagnostic methods to improve testing capacity and reduce result turnaround times, especially in tropical regions where OROV prevalence is rising.To develop and validate a rapid, cost-effective diagnostic assay for OROV detection suitable for use in ...
Livia do Carmo Silva +7 more
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