Results 131 to 140 of about 2,171,089 (338)

Sensitivity and reproducibility of standardized-competitive RT-PCR for transcript quantification and its comparison with real time RT-PCR

Molecular Cancer, 2004
Background Probe based detection assays form the mainstay of transcript quantification. Problems with these assays include varying hybridization efficiencies of the probes used for transcript quantification and the expense involved.
Willey James, Cai Jie, Laird Peter W, Groshen Susan, Beil Stephen J, George Ben, Pagliarulo Vincenzo, Cote Richard J, Datar Ram H   +8 more
doaj  

Short peptide perturbs spermatogenesis via immune microenvironment dysregulation and mitochondrial imbalance

FEBS Open Bio, EarlyView.
In the blood–testis barrier, occludin is crucial for tight junctions. This study demonstrates that occludin‐targeting short peptides disrupt junction integrity, inducing immune cell infiltration, tumor necrosis factor‐α/interleukin‐6 secretion and mitochondrial dysfunction, ultimately triggering apoptosis.
Heng Wang, Xiaofang Tan, Deyu Chen
wiley   +1 more source

Improving the Response rate of RT PCR testing using deep convolutional encoder

Measurement: Sensors, 2023
The sensitivity of the RT-PCR test is limited, and the method itself is laborious and time-consuming to carry out. When it comes to making a diagnosis of COVID-19, chest CT scans have the potential to be of assistance.
I. Shanmugapriya, D. Sowmya Devi
doaj  

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags [PDF]

, 1999
Anke van den Berg
openalex   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

Multiplex PCR for quality control of template RNA/cDNA in RT-PCR assays [PDF]

, 1998
Franz Watzinger, Thomas Lion
openalex   +1 more source

Co‐overexpression of the caloric restriction‐induced mitochondrial factors PGC‐1α and MIPEP upregulates Phospho1 expression in adipocytes

FEBS Open Bio, EarlyView.
Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu, Kanari Taki, Asuka Hayami, Komachi Kato, Yuka Nozaki, Yuhei Mizunoe, Takumi Narita, Ryoichi Mori, Yoshikazu Higami, Masaki Kobayashi   +9 more
wiley   +1 more source

The cochaperone BAG3 promotes the stabilization of p53 under heat stress conditions

FEBS Open Bio, EarlyView.
Under heat stress, BAG3 translocates to the nucleus and forms a complex with Hsp70 and p53, thereby promoting p53 stabilization and enhancing its transcriptional activity. These findings suggest that BAG3 functions as a cochaperone that supports p53‐mediated stress responses in cooperation with Hsp70.
Ngoc Nguyen Thi Minh, Esther Lee, Soo‐A Kim   +2 more
wiley   +1 more source

Interphase cytogenetics and competitive RT‐PCR for residual disease monitoring in patients with chronic myeloid leukaemia during interferon‐α therapy [PDF]

, 1998
Andréï Tchirkov, M. Giollant, F Tavernier, G. Briançon, Olivier Tournilhac, Fabrice Kwiatkowski, Pierre Philippe, Bachra Choufi, François Deméocq, Philippe Travade, P Malet   +10 more
openalex   +1 more source