Results 301 to 310 of about 2,171,089 (338)
Author Correction: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR. [PDF]
Sci RepFazzina M +5 more
europepmc +1 more source
Investigating the relationship between cycle threshold of SARS-CoV-2 RT-PCR, clinical features, and laboratory data in hospitalized COVID-19 patients in Semnan, Iran. [PDF]
Front Cell Infect MicrobiolKalantar Neyestanaki MH +7 more
europepmc +1 more source
Detection of Viroids by RT-PCR [PDF]
, 2021 Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels.
Mengji Cao +2 more
openaire +2 more sources
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2021
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco Faggioli, Marta Luigi
openaire +3 more sources
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco Faggioli, Marta Luigi
openaire +3 more sources
Current Protocols in Cell Biology, 2001
AbstractThis unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).
Lu Charboneau +2 more
openaire +3 more sources
AbstractThis unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).
Lu Charboneau +2 more
openaire +3 more sources
Journal of Virological Methods, 2000
Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
Fred Brown +3 more
openaire +3 more sources
Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
Fred Brown +3 more
openaire +3 more sources
Archives of Academic Emergency Medicine, 2020
Introduction: The role of laboratory parameters in screening of COVID-19 cases has not been definitely established. This study aimed to evaluate the accuracy of laboratory parameters in predicting cases with positive RT-PCR for COVID-19.
R. Mardani +7 more
semanticscholar +1 more source
Introduction: The role of laboratory parameters in screening of COVID-19 cases has not been definitely established. This study aimed to evaluate the accuracy of laboratory parameters in predicting cases with positive RT-PCR for COVID-19.
R. Mardani +7 more
semanticscholar +1 more source