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A sensitive multiplex RT-qPCR assay to detect SARS-CoV-2 in respiratory samples

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Detection of Viroids Using RT-qPCR

2021
Viroids are the smallest known infectious pathogens. They are nonprotein-encoding, single-stranded, circular, naked RNA molecules that can cause several diseases in economically important crops. With the advent of thermal cyclers incorporating fluorescent detection, reverse transcription coupled to the quantitative polymerase chain reaction (RT-qPCR ...
Subhas, Hajeri   +2 more
openaire   +2 more sources

Novel duplex RT‐qPCR for animal rabies surveillance

Transboundary and Emerging Diseases, 2022
Rabies is a lethal zoonosis affecting mammals worldwide. Diagnosis of rabies follows international standard protocols, primarily relying on direct immunofluorescence (DI) followed by mouse inoculation test (MIT). WHO recommends molecular biology techniques such as RT-qPCR for replacing MIT to diagnose rabies in animal samples. Recently, a real-time PCR
Guilherme Augusto Minozzo   +7 more
openaire   +2 more sources

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