Results 141 to 150 of about 317,828 (311)

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

open access: yesCytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen   +2 more
wiley   +1 more source

Engineering and Characterization of a Fluorescent Fission Yeast Arp2/3 Complex for Single Molecule Mechanistic Investigations

open access: yesCytoskeleton, EarlyView.
ABSTRACT Arp2/3 complex is a seven‐component protein complex that facilitates the assembly of branched actin filament networks by binding to pre‐existing “mother” actin filaments and initiating the nucleation of new branched “daughter” filaments. Arp2/3 complex must be activated by a nucleation promoting factor such as the WASP/Scar protein family. The
Caitlin A. Anderson   +8 more
wiley   +1 more source

Microtubule Destabilizing Kinesins: A Historical Perspective and Thoughts for the Future

open access: yesCytoskeleton, EarlyView.
ABSTRACT The microtubule cytoskeleton plays critical roles in multiple cellular processes such as mitotic spindle assembly and chromosome segregation. Cells contain a number of microtubule‐associated proteins that regulate microtubule dynamics both temporally and spatially in cells. The microtubule depolymerizing kinesins include members of the Kinesin‐
Claire E. Walczak
wiley   +1 more source

Quantifying Actin Persistence Length in S. pombe and S. cerevisiae: The Impact of Data Size, Filament Length and Fitting Strategy

open access: yesCytoskeleton, EarlyView.
ABSTRACT Actin filaments exhibit isoform and species‐specific mechanical properties that are commonly quantified via their persistence length, yet the reliability of such measurements depends not only on the analysis method but also on dataset characteristics.
Peter Nietmann   +3 more
wiley   +1 more source

Benchmarking of Fluorescent Proteins in Trichoderma reesei Using Flow Cytometry

open access: yesCytometry Part A, EarlyView.
ABSTRACT Fungi are the subject of extensive research due to their significant impact on human life. However, the development of tools based on fluorescent proteins has been slow in these species. Trichoderma reesei, a filamentous fungus known for its high secretion capability, shows great potential for producing heterologous proteins.
Métivier Killian   +3 more
wiley   +1 more source

Antimicrobial Effects of Ocimum gratissimum Extracts on the Spoilage Organisms Isolated from Yoghurt Samples

open access: yesUMYU Journal of Microbiology Research
Ocimum gratissimum (OG) is recognised for its nutritional, antioxidant, and antimicrobial properties, making it a strong candidate for natural food preservation. This study evaluated the effects of ethanolic and aqueous OG leaf extracts against spoilage
Olawumi Amupitan Adefunke   +3 more
doaj   +1 more source

Sex and Species differentiation in Saccharomyces yeasts [PDF]

open access: yes, 1990
P(論文)We isolated heterothallic haploid strains from a homothallic diploid strain of a yeast Saccharomyces exiguus by mutagenization with UV or ethylmethanesulfonate(EMS).
42044, 41963, 久冨, 泰資
core  

Secretopathies emerge as a new class of neurocristopathies

open access: yesDevelopmental Dynamics, EarlyView.
Abstract Neural crest cells are a transient embryonic population of cells that give rise to a wide range of structures, including craniofacial cartilage and bone, peripheral neurons and glia, as well as components of the cardiac outflow tract, among others.
Amanda Teixeira   +3 more
wiley   +1 more source

Análise do controle metabólico in silico da via glicolítica da Saccharomyces cerevisiae [PDF]

open access: yes, 2015
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Química, Florianópolis, 2015.Saccharomyces cerevisiae é o principal micro-organismo produtor de etanol, empregado no Brasil ...
Aguiar, Rodrigo Souza
core  

Putting System Co‐Design at the Center of Chemo‐Biohybrid CO2 Valorization From Lab to Industrial Scale

open access: yesEcoEnergy, EarlyView.
Chemo‐biohybrid systems promise much, but single‐discipline research still limits progress. This perspective draws on thermocatalytic, electrocatalytic, and photocatalytic hybrid systems to highlight the need for system‐level co‐design and outline future directions.
Wenwen Yu, Hanjun Sun, Long Liu
wiley   +1 more source

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