Biotin Protein Ligase from Saccharomyces cerevisiae [PDF]
Journal of Biological Chemistry, 1999 Catalytically active biotin protein ligase from Saccharomyces cerevisiae (EC 6.3.4.15) was overexpressed in Escherichia coli and purified to near homogeneity in three steps. Kinetic analysis demonstrated that the substrates ATP, biotin, and the biotin-accepting protein bind in an ordered manner in the reaction mechanism.
Polyak, S. +3 more
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Construction of a Plasmodium falciparum Rab-interactome identifies CK1 and PKA as Rab-effector kinases in malaria parasites [PDF]
, 2011 Background information The pathology causing stages of the human malaria parasite Plasmodium falciparum reside within red blood cells that are devoid of any regulated transport system.
Bourdoncle, P. +9 more
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The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway [PDF]
, 2009 © 2009 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,.
Höfken, Thomas +5 more
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How to halve ploidy : lessons from budding yeast meiosis [PDF]
, 2012 Maintenance of ploidy in sexually reproducing organisms requires a specialized form of cell division called meiosis that generates genetically diverse haploid gametes from diploid germ cells.
Arumugam, Prakash +2 more
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Identification and functional analysis of novel phosphorylation sites in the RNA surveillance protein Upf1. [PDF]
, 2013 One third of inherited genetic diseases are caused by mRNAs harboring premature termination codons as a result of nonsense mutations. These aberrant mRNAs are degraded by the Nonsense-Mediated mRNA Decay (NMD) pathway.
Bracho, Dina P +11 more
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Prion protein gene polymorphisms in Saccharomyces cerevisiae [PDF]
Molecular Microbiology, 2003 SummaryThe yeast Saccharomyces cerevisiae genome encodes several proteins that, in laboratory strains, can take up a stable, transmissible prion form. In each case, this requires the Asn/Gln‐rich prion‐forming domain (PrD) of the protein to be intact.
Resende, Catarina +4 more
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A novel platform for the production of nonhydroxylated gelatins based on the methylotrophic yeast Hansenula polymorpha [PDF]
, 2007 The use of yeast as a host for heterologous expression of proteins that are normally derived from animal tissue is a promising way to ensure defined products that are devoid of potential harmful animal side products.
Boer, Arjo L. de, +4 more
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Pathways and Mechanisms that Prevent Genome Instability in Saccharomyces cerevisiae. [PDF]
, 2017 Genome rearrangements result in mutations that underlie many human diseases, and ongoing genome instability likely contributes to the development of many cancers.
Kolodner, Richard D +1 more
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Tor1 regulates protein solubility in Saccharomyces cerevisiae
Molecular Biology of the Cell, 2012 Accumulation of insoluble protein in cells is associated with aging and aging-related diseases; however, the roles of insoluble protein in these processes are uncertain. The nature and impact of changes to protein solubility during normal aging are less well understood.
Peters, Theodore W. +8 more
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Impact of limited solvent capacity on metabolic rate, enzyme activities, and metabolite concentrations of S. cerevisiae glycolysis [PDF]
, 2008 The cell's cytoplasm is crowded by its various molecular components, resulting in a limited solvent capacity for the allocation of new proteins, thus constraining various cellular processes such as metabolism.
Barabási, AL +3 more
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