Results 71 to 80 of about 274,580 (343)

Diet supplementation with Saccharomyces cerevisiae influences the electrophoretic parameters in blood in young Charolaise bulls

open access: yesJournal of Veterinary Research
The objective of the research was to investigate the effect of Saccharomyces cerevisiae supplementation on some acute-phase proteins, haptoglobin and all electrophoretic parameters in young Charolaise bulls.
Arfuso Francesca   +6 more
doaj   +1 more source

A flexible protein linker improves the function of epitope‐tagged proteins in Saccharomyces cerevisiae

open access: yesYeast, 2007
Epitope tagging permits the detection of proteins when protein‐specific antibodies are not available. However, the epitope tag can reduce the function of the tagged protein.
M. Sabourin   +3 more
semanticscholar   +1 more source

Computationally Guided Liquid Crystal‐Based Competitive Binding Sensing Platform for Optical Detection of Spike Protein

open access: yesAdvanced Materials Technologies, EarlyView.
A competitive‐binding paradigm for detection of viruses with the detection of the receptor binding domain of the spike protein as an illustrative example is introduced. Importantly, this approach does not rely on antibodies and therefore is of significance for detecting novel and emerging pathogens. Abstract A liquid crystal (LC)‐based sensing platform
Homa Ghaiedi   +4 more
wiley   +1 more source

Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae

open access: yesBio-Protocol, 2018
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters.
Sviatlana Shashkova   +3 more
doaj   +1 more source

Genome-wide analyses and functional classification of proline repeat-rich proteins: potential role of eIF5A in eukaryotic evolution. [PDF]

open access: yesPLoS ONE, 2014
The eukaryotic translation factor, eIF5A has been recently reported as a sequence-specific elongation factor that facilitates peptide bond formation at consecutive prolines in Saccharomyces cerevisiae, as its ortholog elongation factor P (EF-P) does in ...
Ajeet Mandal   +2 more
doaj   +1 more source

Saccharomyces cerevisiae Pex3p and Pex19p are required for proper localization and stability of peroxisomal membrane proteins

open access: yesEMBO Journal, 2000
The mechanisms by which peroxisomal membrane proteins (PMPs) are targeted to and inserted into membranes are unknown, as are the required components. We show that among a collection of 16 Saccharomyces cerevisiae peroxisome biogenesis (pex) mutants, two ...
E. Hettema   +4 more
semanticscholar   +1 more source

Genome‐Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells

open access: yesAdvanced Science, EarlyView.
This study utilized Ss‐STARR‐seq to construct a genome‐wide silencer map of mouse cells and elucidated the fundamental characteristics of these silencers. These silencers exist in a cell‐specific manner and exhibit unique epigenetic modifications. Through functional experiments, we also discovered that these silencers can regulate gene expression and ...
Xiusheng Zhu   +12 more
wiley   +1 more source

Destabilization of mutated human PUS3 protein causes intellectual disability

open access: yesHuman Mutation, Volume 43, Issue 12, Page 2063-2078, December 2022., 2022
The homodimer of pseudouridine synthase 3 converts uridines at position 39 in tRNAs to pseudouridine (ψ), which affects tRNA stability and regulates RNA–protein interactions. Several patient‐derived variants of PUS3 have been associated with neurodegenerative diseases.
Ting‐Yu Lin   +17 more
wiley   +1 more source

Following the flux of long-chain bases through the sphingolipid pathway in vivo using mass spectrometry[S]

open access: yesJournal of Lipid Research, 2016
Sphingolipids are essential components of the plasma membrane. Their synthesis is tightly controlled by regulatory proteins, which impinge on the rate-limiting step of the pathway, the condensation of serine and palmitoyl-CoA to long-chain base (LCB ...
Fernando Martínez-Montañés   +1 more
doaj  

Macromolecular protein crystallisation with biotemplate of live cells

open access: yesScientific Reports, 2022
Macromolecular protein crystallisation was one of the potential tools to accelerate the biomanufacturing of biopharmaceuticals. In this work, it was the first time to investigate the roles of biotemplates, Saccharomyces cerevisiae live cells, in the ...
Mubai Sun   +4 more
doaj   +1 more source

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