Results 1 to 10 of about 488 (105)

Model‐Guided Systematic Metabolic Engineering for Enhanced Spinosad Biosynthesis in Saccharopolyspora spinosa NHF132 [PDF]

Advanced Science
Spinosad (a mixture of spinosyns A and D) is a macrocyclic lactone green bioinsecticide produced by Saccharopolyspora spinosa. It is known for its high efficiency, low toxicity, and broad‐spectrum activity. Although numerous strategies have been employed
Shuliu Wang, Yuxin Liu, Qian Zhang, Yue Jiang, Xiaoqian Zeng, Chengyu Zhang, Jiagao Cheng, Weishan Wang, Lixin Zhang, Gao‐Yi Tan   +9 more
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Systematic Engineering of Proteases in Saccharopolyspora Spinosa Reveals Synergistic Enhancement of Spinosad Biosynthesis via Substrate Flux Optimization [PDF]

Advanced Science
Natural product biosynthesis is tightly coupled to nitrogen allocation. However, scalable strategies to amplify pathway outputs remain limited. Here, atmospheric and room temperature plasma (ARTP) mutagenesis of Saccharopolyspora spinosa yielded a high ...
Duo Jin, Wangqiong Chen, Danlu Yang, Qing Liu, Baolong Bai, Zirui Dai, Xirong Liu, Zirong Zhu, Jie Rang, Liqiu Xia   +9 more
doaj   +3 more sources

Effects of acuC on the growth development and spinosad biosynthesis of Saccharopolyspora spinosa [PDF]

Microbial Cell Factories, 2021
Background Acetoin utilization protein (acuC) is a type I histone deacetylase which is highly conserved in bacteria. The acuC gene is related to the acetylation/deacetylation posttranslational modification (PTM) system in S. spinosa.
Zhudong Liu, Jie Xiao, Jianli Tang, Yang Liu, Ling Shuai, Li Cao, Ziyuan Xia, Xuezhi Ding, Jie Rang, Liqiu Xia   +9 more
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Comparative transcriptomic analysis of two Saccharopolyspora spinosa strains reveals the relationships between primary metabolism and spinosad production [PDF]

Scientific Reports, 2021
Saccharopolyspora spinosa is a well-known actinomycete for producing the secondary metabolites, spinosad, which is a potent insecticides possessing both efficiency and safety.
Yunpeng Zhang, Xiaomeng Liu, Tie Yin, Qi Li, Qiulong Zou, Kexue Huang, Dongsheng Guo, Xiaolin Zhang   +7 more
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Deciphering the Metabolic Pathway Difference Between Saccharopolyspora pogona and Saccharopolyspora spinosa by Comparative Proteomics and Metabonomics [PDF]

Frontiers in Microbiology, 2020
Butenyl-spinosyn, a secondary metabolite produced by Saccharopolyspora pogona, exhibits strong insecticidal activity than spinosyn. However, the low synthesis capacity and unknown metabolic characteristics of butenyl-spinosyn in wild-type S. pogona limit
Jie Rang, Haocheng He, Shuangqin Yuan, Jianli Tang, Zhudong Liu, Ziyuan Xia, Tahir Ali Khan, Shengbiao Hu, Ziquan Yu, Yibo Hu, Yunjun Sun, Weitao Huang, Xuezhi Ding, Liqiu Xia   +13 more
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rpoB and efp are stable candidate reference genes for quantitative real-time PCR analysis in Saccharopolyspora spinosa

Biotechnology and Biotechnological Equipment, 2021
Spinosad (spinosyn A and spinosyn D), the secondary metabolite produced by Saccharopolyspora spinosa, is a potent insecticide with low effects on the environment and mammals.
Xiaomeng Liu, Yunpeng Zhang, Kexue Huang, Tie Yin, Qi Li, Qiulong Zou, Dongsheng Guo, Xiaolin Zhang   +7 more
exaly   +2 more sources

Enhancement of spinosad production in Saccharopolyspora spinosa by overexpression of the complete 74-kb spinosyn gene cluster [PDF]

Microbial Cell Factories
Background Spinosad, a secondary metabolite produced by Saccharopolyspora spinosa, is a polyketide macrolide insecticide with low toxicity and environmental friendliness.
Lu Gan, Zhengyu Zhang, Jingtao Chen, Zhichun Shen, Wujie Chen, Shaoxin Chen, Jiyang Li   +6 more
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Chemical composition and bioinsecticidal activity of bioinputs produced by Saccharopolyspora spinosa [PDF]

Applied Microbiology and Biotechnology
The growing demand for sustainable agricultural pest control solutions has increased the use of microbial bioinput. This study characterized an industrial bioinput produced by Saccharopolyspora spinosa, focusing on identifying metabolites and quantifying
Luís Felipe Rodrigues Costa, Amanda Evelyn Miranda, Márliton Pereira dos Santos, Jorge Luiz Soares dos Anjos, Fernanda Menezes Maia, Tiara da Costa Silva, Mario Machado Martins, Joseilton Faria Silva, Isac Pereira Soares Martins, Clarice Diniz Alvarenga, Marlon Cristian Toledo Pereira, Nelson de Abreu Delvaux Júnior, Luciano Pereira Rodrigues   +12 more
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CRISPRi-mediated multigene downregulating redirects the metabolic flux to spinosad biosynthesis in Saccharopolyspora spinosa [PDF]

Synthetic and Systems Biotechnology
Microorganisms are often likened to complex production workshops. In Saccharopolyspora spinosa (S. spinosa), the biosynthesis of spinosad is a production line within its intricate workshop.
Zirong Zhu, Li Cao, Ziyuan Xia, Xirong Liu, Wangqion Chen, Zirui Dai, Duo Jin, Jie Rang, Shengbiao Hu, Liqiu Xia   +9 more
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Enhanced triacylglycerol metabolism contributes to the efficient biosynthesis of spinosad in Saccharopolyspora spinosa [PDF]

Synthetic and Systems Biotechnology
Triacylglycerol (TAG) is crucial for antibiotic biosynthesis derived from Streptomyces, as it serves as an important carbon source. In this study, the supplementation of exogenous TAG led to a 3.92-fold augmentation in spinosad production.
Li Cao, Yangchun Liu, Lin Sun, Zirong Zhu, Danlu Yang, Ziyuan Xia, Duo Jin, Zirui Dai, Jie Rang, Liqiu Xia   +9 more
doaj   +2 more sources