Results 211 to 220 of about 2,377,512 (309)

Erratum for CySP3-96 Enables Scalable, Streamlined, and Low-Cost Sample Preparation for Cysteine Chemoproteomic Applications. [PDF]

Mol Cell Proteomics
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

High-Throughput Proteomics Sample Preparation Using a 96-Channel Pipettor and Magnetic Pin Device. [PDF]

J Proteome Res
Roumelioti G, Montoya A, Fisher GLM, Navarro EP, Woods A, Bennett J, Navaratnam N, Gonzalez-Carvajal O, Birch J, Pyman E, Yu S, Gruevska A, Vuillemenot LA, Lushchak O, Hall Z, Barr AR, Speck C, Vernia S, Scott WR, Gil J, Aragon L, Fets L, Carling D, Shliaha PV.   +23 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Shake and bake: a robust and cost-effective proteomic sample preparation workflow for plasma and cerebrospinal fluid. [PDF]

Clin Proteomics
Rönnholm J, Weiner S, Fuchs J, Thorsell A, Sihlbom C, Dugom L, Easton A, Zetterberg H, Gobom J.   +8 more
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Impact of Preanalytical Sample Preparation on the Recovery Rate of Cryopreserved Allogeneic Hematopoietic Stem Cells. [PDF]

Eur J Haematol
Blüm P, Glas C, Bieback K, Kayser S, Klüter H, Wuchter P.   +5 more
europepmc   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Design and evaluation of low-cost, DIY programmable tissue processor for solvent exchange in biological sample preparation. [PDF]

PLoS One
Wang M, Pelletier S, Ellis A, Shepherd RF, Frank MH, Stroock AD, Mishra AK, Bacheva V.   +7 more
europepmc   +1 more source

Improving sample preparation by biochar-coated sampling tubes: proof-of-concept extraction of sex hormones from real waters [PDF]

Quarta V., Milanese C., Ferrari L., Merlo F., Profumo A., Speltini A., Bianchini P.   +6 more
core   +1 more source