Advanced Science, EarlyView.Here, we present SensEnhance, a modular genetic circuit that converts leaky, low‐dynamic‐range promoter signals into tightly controlled, high‐dynamic‐range outputs. Applied to mechanosensing and IL‐6‐responsive promoters, SensEnhance increases fold changes from 2.6‐ and 2.5‐fold in promoter‐only configurations to over 20‐ and 14‐fold, respectively ...
Miguel Baños +4 more
wiley +1 more source
Genotyping of human isolates from human toxoplasmosis outbreak: Restriction Fragment Length Polymorphism and Sanger Sequencing. [PDF]
Braz J MicrobiolFernandes FD +6 more
europepmc +1 more source
Rare Differentiation in Genes XIRP1 and XIRP2 Associated With Hypertrophic Cardiomyopathy
AGING MEDICINE, EarlyView.XIRP2, but not XIRP1, acts as a key genetic modifier in hypertrophic cardiomyopathy (HCM). Rare variants in XIRP2 are enriched in patients and lead to dramatic mRNA overexpression (> 9‐fold, p < 0.001). Functionally, this disrupts intercalated disc integrity.
Fang Wang +12 more
wiley +1 more source
isolateR: an R package for generating microbial libraries from Sanger sequencing data. [PDF]
BioinformaticsDaisley B +11 more
europepmc +1 more source
Annals of Gastroenterological Surgery, EarlyView.KRAS mutation demonstrated a prognostic impact according to primary tumor sidedness, independent of tumor morphology, in patients undergoing hepatectomy for colorectal liver metastases. A nomogram integrating biological and clinicopathological factors was developed to provide continuous biological risk assessment.
Yuzo Umeda +9 more
wiley +1 more source
Majority‐Voting Overlapping Method for Error Correction in DNA Data Storage
Advanced Intelligent Discovery, EarlyView.We propose an overlapping‐based majority‐voting method for DNA data storage error correction. By aligning multiple reads and choosing the most frequent base per position, it suppresses substitution errors without prior models. Validated on synthetic and real sequencing data, it achieves high‐fidelity, scalable, and cost‐effective reconstruction ...
Thi Bich Ngoc Nguyen +5 more
wiley +1 more source
Correction: Biba et al. A Comparison of Sanger Sequencing and Amplicon-Based Next Generation Sequencing Approaches for the Detection of HIV-1 Drug Resistance Mutations. Viruses 2024, 16, 1465. [PDF]
VirusesBiba C +7 more
europepmc +1 more source
Advanced Intelligent Systems, Volume 7, Issue 3, March 2025.Quantitative phase maps of single cells recorded in flow cytometry modality feed a hierarchical architecture of machine learning models for the label‐free identification of subtypes of ovarian cancer. The employment of a priori clinical information improves the classification performance, thus emulating the clinical application of liquid biopsy during ...
Daniele Pirone +11 more
wiley +1 more source
Gene variants of interferon induced with helicase C domain 1 in Japanese patients with Dermatomyositis-associated rapidly progressive interstitial lung disease: a genetic association study using whole-exome and Sanger sequencing. [PDF]
Respir ResOkamoto M +18 more
europepmc +1 more source
Concurrent Germline RB1 & Mosaic TP53 in a Child With Multiple Childhood Cancers
American Journal of Medical Genetics Part A, EarlyView.ABSTRACT We report a patient with a pathogenic germline variant (PGV) in RB1 and somatic mosaicism for a pathogenic TP53 variant who developed three distinct types of childhood cancer: retinoblastoma, osteosarcoma, and myelodysplastic syndrome (MDS) before the age of 6 years.
Ole Haubjerg Nielsen +8 more
wiley +1 more source