Results 41 to 50 of about 224,524 (254)

Introduction of the hybcell-based compact sequencing technology and comparison to state-of-the-art methodologies for KRAS mutation detection

BioTechniques, 2015
The detection of KRAS mutations in codons 12 and 13 is critical for anti-EGFR therapy strategies; however, only those methodologies with high sensitivity, specificity, and accuracy as well as the best cost and turnaround balance are suitable for routine ...
Agnes Zopf, Roman Raim, Martin Danzer, Norbert Niklas, Rita Spilka, Johannes Pröll, Christian Gabriel, Andreas Nechansky, Markus Roucka   +8 more
doaj   +1 more source

MutAid: Sanger and NGS Based Integrated Pipeline for Mutation Identification, Validation and Annotation in Human Molecular Genetics. [PDF]

PLoS ONE, 2016
Traditional Sanger sequencing as well as Next-Generation Sequencing have been used for the identification of disease causing mutations in human molecular research.
Ram Vinay Pandey, Stephan Pabinger, Albert Kriegner, Andreas Weinhäusel   +3 more
doaj   +1 more source

The First Sequence: Fred Sanger and Insulin [PDF]

Genetics, 2002
FRED Sanger is an amazingly modest man, and his own retrospective, written after he retired, a delightful prefatory chapter for the Annual Reviews of Biochemistry, is called “Sequences, sequences, and sequences” ([Sanger 1988][1]).
openaire   +2 more sources

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Comparison of clinical metagenomics with 16S rDNA Sanger sequencing for the bacteriological diagnosis of culture-negative samples

International Journal of Medical Microbiology
Background: Currently, diagnosis of bacterial infections is based on culture, possibly followed by the amplification and sequencing (Sanger method) of the 16S rDNA - encoding gene when cultures are negative. Clinical metagenomics (CMg), i.e.
Camille d’Humières, Skerdi Haviari, Marie Petitjean, Laurène Deconinck, Signara Gueye, Nathan Peiffer-Smadja, Lynda Chalal, Naima Beldjoudi, Geoffrey Rossi, Yann Nguyen, Charles Burdet, Ségolène Perrineau, Diane Le Pluart, Roza Rahli, Michael Thy, Piotr Szychowiak, Xavier Lescure, Véronique Leflon-Guibout, Victoire de Lastours, Etienne Ruppé   +19 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Two Different MLC1 Variants Compounded with a Common Variant S93L in Japanese Patients of Megalencephalic Leukoencephalopathy with Subcortical Cysts

Tokyo Women's Medical University Journal, 2020
Megalencephalic leukoencephalopathy with subcortical cysts (MLC; MIM#604004) is a rare congenital disorder of the cerebral white matter. Most MLC patients have MLC1 mutations which are inherited in an autosomal recessive manner.
Keiko Yamamoto-Shimojima, Yasuhiro Kimoto, Yoshiyuki Watanabe, Toshiyuki Yamamoto   +3 more
doaj   +1 more source

Fred Sanger: sequencing pioneer [PDF]

Biochemical Journal, 2005
Fred Sanger is a biochemist who practised chemistry and whose discoveries have been fundamental to the development of modern biological science. His insights into sequencing methods and the central roles played by proteins and nucleic acids in the molecular processes of biology led him to dedicate a lifetime to their investigation.
openaire   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

FEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito, Haduki Asano, Ayano Ueki, Joe Sakamoto, Yasuhiro Kamei, Hayato Tokumoto, Ayaka Yazawa, Shigeki Kamitani   +7 more
wiley   +1 more source